公开(公告)号：US07629176B2

公开(公告)日：2009-12-08

申请号：US12029289

申请日：2008-02-11

申请人： Thomas Haubert ,  Vince Contini ,  Steve Grimes ,  Randy Jones ,  Stephen C. Wardlaw

发明人： Thomas Haubert ,  Vince Contini ,  Steve Grimes ,  Randy Jones ,  Stephen C. Wardlaw

IPC分类号： G01N1/18 ,  G01N33/48

CPC分类号： G01N33/5094 ,  B01L3/50215 ,  G01N33/491 ,  Y10T436/111666 ,  Y10T436/25 ,  Y10T436/25375

摘要： A tube and float system for use in separation and axial expansion of the buffy coat includes a transparent or semi-transparent, flexible sample tube and a rigid separator float having a specific gravity intermediate that of red blood cells and plasma. The float includes a main body portion of reduced diameter to provide a clearance gap between the inner wall of the sample tube and the float. One or more protrusions on the main body portion serve to support the flexible tube. During centrifugation, the centrifugal force causes the diameter of the flexible tube to expand and permit density-based axial movement of the float in the tube. The float further includes a pressure relief system to alleviate pressure build up in the trapped red blood cell blood fraction below the float, thereby preventing red blood cells from being forced into the annular gap containing the buffy coat layers.

摘要翻译： 用于血沉棕黄层的分离和轴向膨胀的管和浮子系统包括透明或半透明的柔性样品管和具有红细胞和血浆的比重中间体的刚性分离器浮子。 浮子包括直径减小的主体部分，以在样品管的内壁和浮子之间提供间隙。 主体部分上的一个或多个突起用于支撑柔性管。 在离心过程中，离心力使柔性管的直径膨胀并允许管中的浮子的基于密度的轴向运动。 浮子还包括一个压力释放系统，以减轻在浮子下面的被捕获的红细胞血液部分中的压力增加，从而防止红细胞被迫进入含有血沉棕黄层的环形间隙。

公开(公告)号：US08114680B2

公开(公告)日：2012-02-14

申请号：US13052172

申请日：2011-03-21

申请人： Thomas Haubert ,  Vince Contini ,  Steve Grimes ,  Randy Jones ,  Stephen C. Wardlaw

发明人： Thomas Haubert ,  Vince Contini ,  Steve Grimes ,  Randy Jones ,  Stephen C. Wardlaw

IPC分类号： G01N1/18 ,  G01N33/48 ,  C12M1/34

CPC分类号： G01N33/5094 ,  B01L3/50215 ,  G01N33/491 ,  Y10T436/111666 ,  Y10T436/25 ,  Y10T436/25375

摘要： A tube and float system for use in separation and axial expansion of the buffy coat includes a transparent or semi-transparent, flexible sample tube and a rigid separator float having a specific gravity intermediate that of red blood cells and plasma. The float includes a main body portion of reduced diameter to provide a clearance gap between the inner wall of the sample tube and the float. One or more protrusions on the main body portion serve to support the flexible tube. During centrifugation, the centrifugal force causes the diameter of the flexible tube to expand and permit density-based axial movement of the float in the tube. The float further includes a pressure relief system to alleviate pressure build up in the trapped red blood cell blood fraction below the float, thereby preventing red blood cells from being forced into the annular gap containing the buffy coat layers.

摘要翻译： 用于血沉棕黄层的分离和轴向膨胀的管和浮子系统包括透明或半透明的柔性样品管和具有红细胞和血浆的比重中间体的刚性分离器浮子。 浮子包括直径减小的主体部分，以在样品管的内壁和浮子之间提供间隙。 主体部分上的一个或多个突起用于支撑柔性管。 在离心过程中，离心力使柔性管的直径膨胀并允许管中的浮子的基于密度的轴向运动。 浮子还包括一个压力释放系统，以减轻在浮子下面的被捕获的红细胞血液部分中的压力增加，从而防止红细胞被迫进入含有血沉棕黄层的环形间隙。

公开(公告)号：US20110165672A1

公开(公告)日：2011-07-07

申请号：US13052172

申请日：2011-03-21

申请人： Thomas Haubert ,  Vince Contini ,  Steve Grimes ,  Randy Jones ,  Stephen C. Wardlaw

发明人： Thomas Haubert ,  Vince Contini ,  Steve Grimes ,  Randy Jones ,  Stephen C. Wardlaw

IPC分类号： C12N5/078 ,  C12M1/34

CPC分类号： G01N33/5094 ,  B01L3/50215 ,  G01N33/491 ,  Y10T436/111666 ,  Y10T436/25 ,  Y10T436/25375

摘要： A tube and float system for use in separation and axial expansion of the buffy coat includes a transparent or semi-transparent, flexible sample tube and a rigid separator float having a specific gravity intermediate that of red blood cells and plasma. The float includes a main body portion of reduced diameter to provide a clearance gap between the inner wall of the sample tube and the float. One or more protrusions on the main body portion serve to support the flexible tube. During centrifugation, the centrifugal force causes the diameter of the flexible tube to expand and permit density-based axial movement of the float in the tube. The float further includes a pressure relief system to alleviate pressure build up in the trapped red blood cell blood fraction below the float, thereby preventing red blood cells from being forced into the annular gap containing the buffy coat layers.

摘要翻译： 用于血沉棕黄层的分离和轴向膨胀的管和浮子系统包括透明或半透明的柔性样品管和具有红细胞和血浆的比重中间体的刚性分离器浮子。 浮子包括直径减小的主体部分，以在样品管的内壁和浮子之间提供间隙。 主体部分上的一个或多个突起用于支撑柔性管。 在离心过程中，离心力使柔性管的直径膨胀并允许管中的浮子的基于密度的轴向运动。 浮子还包括一个压力释放系统，以减轻在浮子下面的被捕获的红细胞血液部分中的压力增加，从而防止红细胞被迫进入含有血沉棕黄层的环形间隙。

公开(公告)号：US20100074803A1

公开(公告)日：2010-03-25

申请号：US12614602

申请日：2009-11-09

申请人： Thomas Haubert ,  Vince Contini ,  Steve Grimes ,  Randy Jones ,  Stephen C. Wardlaw

发明人： Thomas Haubert ,  Vince Contini ,  Steve Grimes ,  Randy Jones ,  Stephen C. Wardlaw

IPC分类号： G01N1/18 ,  G01N33/48

CPC分类号： G01N33/5094 ,  B01L3/50215 ,  G01N33/491 ,  Y10T436/111666 ,  Y10T436/25 ,  Y10T436/25375

摘要： A tube and float system for use in separation and axial expansion of the buffy coat includes a transparent or semi-transparent, flexible sample tube and a rigid separator float having a specific gravity intermediate that of red blood cells and plasma. The float includes a main body portion of reduced diameter to provide a clearance gap between the inner wall of the sample tube and the float. One or more protrusions on the main body portion serve to support the flexible tube. During centrifugation, the centrifugal force causes the diameter of the flexible tube to expand and permit density-based axial movement of the float in the tube. The float further includes a pressure relief system to alleviate pressure build up in the trapped red blood cell blood fraction below the float, thereby preventing red blood cells from being forced into the annular gap containing the buffy coat layers.

摘要翻译： 用于血沉棕黄层的分离和轴向膨胀的管和浮子系统包括透明或半透明的柔性样品管和具有红细胞和血浆的比重中间体的刚性分离器浮子。 浮子包括直径减小的主体部分，以在样品管的内壁和浮子之间提供间隙。 主体部分上的一个或多个突起用于支撑柔性管。 在离心过程中，离心力使柔性管的直径膨胀并允许管中的浮子的基于密度的轴向运动。 浮子还包括一个压力释放系统，以减轻在浮子下面的被捕获的红细胞血液部分中的压力增加，从而防止红细胞被迫进入含有血沉棕黄层的环形间隙。

公开(公告)号：US07919049B2

公开(公告)日：2011-04-05

申请号：US12614602

申请日：2009-11-09

申请人： Thomas Haubert ,  Vince Contini ,  Steve Grimes ,  Randy Jones ,  Stephen C. Wardlaw

发明人： Thomas Haubert ,  Vince Contini ,  Steve Grimes ,  Randy Jones ,  Stephen C. Wardlaw

IPC分类号： G01N1/18 ,  G01N33/48

CPC分类号： G01N33/5094 ,  B01L3/50215 ,  G01N33/491 ,  Y10T436/111666 ,  Y10T436/25 ,  Y10T436/25375

摘要： A tube and float system for use in separation and axial expansion of the buffy coat includes a transparent or semi-transparent, flexible sample tube and a rigid separator float having a specific gravity intermediate that of red blood cells and plasma. The float includes a main body portion of reduced diameter to provide a clearance gap between the inner wall of the sample tube and the float. One or more protrusions on the main body portion serve to support the flexible tube. During centrifugation, the centrifugal force causes the diameter of the flexible tube to expand and permit density-based axial movement of the float in the tube. The float further includes a pressure relief system to alleviate pressure build up in the trapped red blood cell blood fraction below the float, thereby preventing red blood cells from being forced into the annular gap containing the buffy coat layers.

摘要翻译： 用于血沉棕黄层的分离和轴向膨胀的管和浮子系统包括透明或半透明的柔性样品管和具有红细胞和血浆的比重中间体的刚性分离器浮子。 浮子包括直径减小的主体部分，以在样品管的内壁和浮子之间提供间隙。 主体部分上的一个或多个突起用于支撑柔性管。 在离心过程中，离心力使柔性管的直径膨胀并允许管中的浮子的基于密度的轴向运动。 浮子还包括一个压力释放系统，以减轻在浮子下面的被捕获的红细胞血液部分中的压力增加，从而防止红细胞被迫进入含有血沉棕黄层的环形间隙。

公开(公告)号：US07358095B2

公开(公告)日：2008-04-15

申请号：US11609186

申请日：2006-12-11

申请人： Thomas Haubert ,  Vince Contini ,  Steve Grimes ,  Randy Jones ,  Stephen C. Wardlaw

发明人： Thomas Haubert ,  Vince Contini ,  Steve Grimes ,  Randy Jones ,  Stephen C. Wardlaw

IPC分类号： G01N1/18 ,  G01N33/48

CPC分类号： G01N33/5094 ,  B01L3/50215 ,  G01N33/491 ,  Y10T436/111666 ,  Y10T436/25 ,  Y10T436/25375

摘要： A tube and float system for use in separation and axial expansion of the buffy coat includes a transparent or semi-transparent, flexible sample tube and a rigid separator float having a specific gravity intermediate that of red blood cells and plasma. The float includes a main body portion of reduced diameter to provide a clearance gap between the inner wall of the sample tube and the float. One or more protrusions on the main body portion serve to support the flexible tube. During centrifugation, the centrifugal force causes the diameter of the flexible tube to expand and permit density-based axial movement of the float in the tube. The float further includes a pressure relief system to alleviate pressure build up in the trapped red blood cell blood fraction below the float, thereby preventing red blood cells from being forced into the annular gap containing the buffy coat layers.

摘要翻译： 用于血沉棕黄层的分离和轴向膨胀的管和浮子系统包括透明或半透明的柔性样品管和具有红细胞和血浆的比重中间体的刚性分离器浮子。 浮子包括直径减小的主体部分，以在样品管的内壁和浮子之间提供间隙。 主体部分上的一个或多个突起用于支撑柔性管。 在离心过程中，离心力使柔性管的直径膨胀并允许管中的浮子的基于密度的轴向运动。 浮子还包括一个压力释放系统，以减轻在浮子下面的被捕获的红细胞血液部分中的压力增加，从而防止红细胞被迫进入含有血沉棕黄层的环形间隙。

公开(公告)号：US08415169B2

公开(公告)日：2013-04-09

申请号：US13371761

申请日：2012-02-13

申请人： Thomas Haubert ,  Vince Contini ,  Steve Grimes ,  Randy Jones ,  Stephen C. Wardlaw

发明人： Thomas Haubert ,  Vince Contini ,  Steve Grimes ,  Randy Jones ,  Stephen C. Wardlaw

IPC分类号： G01N1/18 ,  G01N33/48 ,  C12M1/34

CPC分类号： B01L3/50215 ,  Y10T436/111666 ,  Y10T436/25375

摘要： A tube and float system for use in separation and axial expansion of the buffy coat includes a transparent or semi-transparent, flexible sample tube and a rigid separator float having a specific gravity intermediate that of red blood cells and plasma. The float includes a main body portion of reduced diameter to provide a clearance gap between the inner wall of the sample tube and the float. One or more protrusions on the main body portion serve to support the flexible tube. During centrifugation, the centrifugal force causes the diameter of the flexible tube to expand and permit density-based axial movement of the float in the tube. The float further includes a pressure relief system to alleviate pressure build up in the trapped red blood cell blood fraction below the float, thereby preventing red blood cells from being forced into the annular gap containing the buffy coat layers.

公开(公告)号：US20120164683A1

公开(公告)日：2012-06-28

申请号：US13371761

申请日：2012-02-13

申请人： Thomas Haubert ,  Vince Contini ,  Steve Grimes ,  Randy Jones ,  Stephen C. Wardlaw

发明人： Thomas Haubert ,  Vince Contini ,  Steve Grimes ,  Randy Jones ,  Stephen C. Wardlaw

IPC分类号： C12Q1/04 ,  B29C45/00 ,  C12M1/34

CPC分类号： B01L3/50215 ,  Y10T436/111666 ,  Y10T436/25375

摘要： A tube and float system for use in separation and axial expansion of the buffy coat includes a transparent or semi-transparent, flexible sample tube and a rigid separator float having a specific gravity intermediate that of red blood cells and plasma. The float includes a main body portion of reduced diameter to provide a clearance gap between the inner wall of the sample tube and the float. One or more protrusions on the main body portion serve to support the flexible tube. During centrifugation, the centrifugal force causes the diameter of the flexible tube to expand and permit density-based axial movement of the float in the tube. The float further includes a pressure relief system to alleviate pressure build up in the trapped red blood cell blood fraction below the float, thereby preventing red blood cells from being forced into the annular gap containing the buffy coat layers.

摘要翻译： 用于血沉棕黄层的分离和轴向膨胀的管和浮子系统包括透明或半透明的柔性样品管和具有红细胞和血浆的比重中间体的刚性分离器浮子。 浮子包括直径减小的主体部分，以在样品管的内壁和浮子之间提供间隙。 主体部分上的一个或多个突起用于支撑柔性管。 在离心过程中，离心力使柔性管的直径膨胀并允许管中的浮子的基于密度的轴向运动。 浮子还包括一个压力释放系统，以减轻在浮子下面的被捕获的红细胞血液部分中的压力增加，从而防止红细胞被迫进入含有血沉棕黄层的环形间隙。

公开(公告)号：US20080131868A1

公开(公告)日：2008-06-05

申请号：US12029289

申请日：2008-02-11

申请人： Thomas Haubert ,  Vince Contini ,  Steve Grimes ,  Randy Jones ,  Stephen C. Wardlaw

发明人： Thomas Haubert ,  Vince Contini ,  Steve Grimes ,  Randy Jones ,  Stephen C. Wardlaw

IPC分类号： A01N1/02 ,  C12M1/24

CPC分类号： G01N33/5094 ,  B01L3/50215 ,  G01N33/491 ,  Y10T436/111666 ,  Y10T436/25 ,  Y10T436/25375

摘要： A tube and float system for use in separation and axial expansion of the buffy coat includes a transparent or semi-transparent, flexible sample tube and a rigid separator float having a specific gravity intermediate that of red blood cells and plasma. The float includes a main body portion of reduced diameter to provide a clearance gap between the inner wall of the sample tube and the float. One or more protrusions on the main body portion serve to support the flexible tube. During centrifugation, the centrifugal force causes the diameter of the flexible tube to expand and permit density-based axial movement of the float in the tube. The float further includes a pressure relief system to alleviate pressure build up in the trapped red blood cell blood fraction below the float, thereby preventing red blood cells from being forced into the annular gap containing the buffy coat layers.

摘要翻译： 用于血沉棕黄层的分离和轴向膨胀的管和浮子系统包括透明或半透明的柔性样品管和具有红细胞和血浆的比重中间体的刚性分离器浮子。 浮子包括直径减小的主体部分，以在样品管的内壁和浮子之间提供间隙。 主体部分上的一个或多个突起用于支撑柔性管。 在离心过程中，离心力使柔性管的直径膨胀并允许管中的浮子的基于密度的轴向运动。 浮子还包括一个压力释放系统，以减轻在浮子下面的被捕获的红细胞血液部分中的压力增加，从而防止红细胞被迫进入含有血沉棕黄层的环形间隙。

公开(公告)号：US20070092971A1

公开(公告)日：2007-04-26

申请号：US11609186

申请日：2006-12-11

申请人： Thomas Haubert ,  Vince Contini ,  Steve Grimes ,  Randy Jones ,  Stephen Wardlaw

发明人： Thomas Haubert ,  Vince Contini ,  Steve Grimes ,  Randy Jones ,  Stephen Wardlaw

IPC分类号： G01N33/48

CPC分类号： G01N33/5094 ,  B01L3/50215 ,  G01N33/491 ,  Y10T436/111666 ,  Y10T436/25 ,  Y10T436/25375

摘要： A tube and float system for use in separation and axial expansion of the buffy coat includes a transparent or semi-transparent, flexible sample tube and a rigid separator float having a specific gravity intermediate that of red blood cells and plasma. The float includes a main body portion of reduced diameter to provide a clearance gap between the inner wall of the sample tube and the float. One or more protrusions on the main body portion serve to support the flexible tube. During centrifugation, the centrifugal force causes the diameter of the flexible tube to expand and permit density-based axial movement of the float in the tube. The float further includes a pressure relief system to alleviate pressure build up in the trapped red blood cell blood fraction below the float, thereby preventing red blood cells from being forced into the annular gap containing the buffy coat layers.

摘要翻译： 用于血沉棕黄层的分离和轴向膨胀的管和浮子系统包括透明或半透明的柔性样品管和具有红细胞和血浆的比重中间体的刚性分离器浮子。 浮子包括直径减小的主体部分，以在样品管的内壁和浮子之间提供间隙。 主体部分上的一个或多个突起用于支撑柔性管。 在离心过程中，离心力使柔性管的直径膨胀并允许管中的浮子的基于密度的轴向运动。 浮子还包括一个压力释放系统，以减轻在浮子下面的被捕获的红细胞血液部分中的压力增加，从而防止红细胞被迫进入含有血沉棕黄层的环形间隙。