公开(公告)号：US20060063253A1

公开(公告)日：2006-03-23

申请号：US11209137

申请日：2005-08-22

申请人： Thomas Maciag ,  Lori Maciag ,  Vihren Kolev ,  Joseph Verdi

发明人： Thomas Maciag ,  Lori Maciag ,  Vihren Kolev ,  Joseph Verdi

IPC分类号： C12N5/06

CPC分类号： C12N5/0623 ,  C12N2501/42 ,  C12N2501/998

摘要： The present invention relates to methods based on the interactions of thrombin as a biological regulator. More specifically, the invention relates to the interactions of thrombin with regard to Notch signaling, Jagged1, PAR1, and cellular effects mediated thereby. The invention relates to the discovery that thrombin cleaves Jagged1 to produce non-membrane soluble Jagged1 (sJ1). The soluble Jagged1 protein can affect Notch signaling and, among other things, mediate the release of FGF-1 and/or IL-1α from a cell. The invention further relates to the role(s) of thrombin and signaling via Notch proteins and the effect on thrombosis, angiogenesis, and/or differentiation, among other processes. Moreover, the invention relates to discovery that thrombin, sJ1, and TRAP mediate, inter alia, rapid non-classical release of FGF-1, and proteins associated therewith (e.g., p40 Syn1 and S100A13, among others), and the effect growth and proliferation of a stem cell without loss of differentiation potential. Thus, the present invention relates to methods of clonally expanding a pluripotent stem cell while preserving the differentiation potential of the cell, a process termed “stamatogenesis.”

摘要翻译： 本发明涉及基于凝血酶作为生物调节剂的相互作用的方法。 更具体地，本发明涉及凝血酶与Notch信号传导，Jagged1，PAR1以及由此介导的细胞效应的相互作用。 本发明涉及凝血酶切割Jagged1以产生非膜可溶性Jagged1（sJ1）的发现。 可溶性Jagged1蛋白可影响Notch信号传导，其中包括从细胞介导FGF-1和/或IL-1α的释放。 本发明还涉及凝血酶的作用和通过Notch蛋白的信号通路以及其他过程中对血栓形成，血管发生和/或分化的作用。 此外，本发明涉及发现凝血酶，sJ1和TRAP特别介导FGF-1的快速非经典释放和与其相关的蛋白质（例如，p40 Syn1和S100A13等），以及效应生长和 干细胞的增殖而不损失分化潜能。 因此，本发明涉及克隆扩增多能干细胞同时保持细胞的分化潜能的方法，称为“致死生成”的过程。

公开(公告)号：US06558912B1

公开(公告)日：2003-05-06

申请号：US09662831

申请日：2000-09-15

申请人： Philip Barker ,  Joseph Verdi ,  Amir Salehi

发明人： Philip Barker ,  Joseph Verdi ,  Amir Salehi

IPC分类号： G01N33574

CPC分类号： C07K14/475 ,  A61K38/00 ,  C07K16/22 ,  C07K2319/00

摘要： The invention features substantially pure NRAGE polypeptides. The invention also features substantially pure nucleic acids encoding these polypeptides. The polypeptides and nucleic acids of the invention are useful for therapeutic and diagnostic purposes, and for drug discovery.

摘要翻译： 本发明具有基本上纯的NRAGE多肽。 本发明还具有基本上纯的编码这些多肽的核酸。 本发明的多肽和核酸可用于治疗和诊断目的以及药物发现。

公开(公告)号：US06521275B1

公开(公告)日：2003-02-18

申请号：US09680615

申请日：2000-10-06

申请人： Vincent Mercogliano ,  Sam Farella ,  William Verdi ,  Joseph Verdi

发明人： Vincent Mercogliano ,  Sam Farella ,  William Verdi ,  Joseph Verdi

IPC分类号： A23L33409

CPC分类号： A23B4/16 ,  A23V2002/00 ,  A23V2200/10 ,  A23V2250/112 ,  A23V2250/118

摘要： A meat preservation process using a carbon monoxide and helium mixture as a preservant which achieves a limited penetration into the meat such that the color of the meat is not altered after cooking, comprising, applying a vacuum to the meat to remove atmospheric gases from the surface of the meat, introducing the carbon monoxide and helium mixture to achieve a limited penetration of the mixture into the surface of the meat, and applying a second vacuum to the meat.

摘要翻译： 一种使用一氧化碳和氦气混合物作为保存剂的肉类保存方法，其实现了肉中有限的渗透，使得烹饪后肉的颜色不改变，包括：向肉中施加真空以从表面除去大气中的气体 的肉，引入一氧化碳和氦混合物以实现混合物有限地渗入肉的表面，并向肉施加第二个真空。