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    INSECT RESISTANCE MANAGEMENT WITH COMBINATIONS OF CRY1BE AND CRY1F PROTEINS
    2.
    发明申请
    INSECT RESISTANCE MANAGEMENT WITH COMBINATIONS OF CRY1BE AND CRY1F PROTEINS 有权
    CRY1BE和CRY1F蛋白质组合的耐药性管理

    公开(公告)号：US20120311746A1

    公开(公告)日：2012-12-06

    申请号：US13516629

    申请日：2010-12-16

    申请人： Thomas Meade ,  Kenneth Narva ,  Nicholas P. Storer ,  Joel J. Sheets ,  Stephanie L. Burton ,  Aaron T. Woosley

    发明人： Thomas Meade ,  Kenneth Narva ,  Nicholas P. Storer ,  Joel J. Sheets ,  Stephanie L. Burton ,  Aaron T. Woosley

    IPC分类号： A01H5/00 ,  C12N5/10 ,  A01N63/00 ,  A01C11/00 ,  A01N37/18 ,  C12N15/82 ,  A01G1/00 ,  A01H5/10 ,  A01P7/04

    CPC分类号： C12N15/8286 ,  A01N63/02 ,  A01N65/00 ,  Y02A40/162 ,  A01N2300/00

    摘要： The subject invention relates in part to stacking Cry1Be toxins along with Cry1Fa toxins to prevent insects from developing resistance towards either toxin by itself. As discussed in more detail herein, the subject pair of proteins is a particularly advantageous combination, as no other pair of proteins is known to provide high levels of control and non-cross-resistant activity against both Spodoptera frugiperda (FAW) and Ostrinia nubilalis (ECB) insects. This dual, non-cross-resistant activity is also advantageous because it can reduce the number of proteins/genes needed to target these insects with multiple, non-cross-resistant proteins. This can reduce or eliminate the need for refuge acreage. Accordingly, the subject invention also relates generally to using four genes to provide three proteins for non-cross-resistant control of a first insect, and three proteins for non-cross-resistant control of a second insect. In preferred embodiments, the targeted insects are FAW and ECB.

    摘要翻译： 本发明部分涉及将Cry1Be毒素与Cry1Fa毒素一起堆叠以防止昆虫本身对毒素产生抗性。 如本文更详细讨论的那样，本发明的对象蛋白质是特别有利的组合，因为没有其他蛋白质对可以提供针对草地贪夜蛾（Spodoptera frugiperda）（FAW）和鸵鸟鸵鸟（Ostrinia nubilalis）的高水平的控制和非交叉抗性活性 ECB）昆虫。 这种双重非交叉抗性活性也是有利的，因为它可以减少用多种非交叉抗性蛋白质靶向这些昆虫所需的蛋白质/基因的数量。 这可以减少或消除避难所面积的需要。 因此，本发明还通常涉及使用四个基因提供用于第一昆虫的非交叉耐药性控制的三种蛋白质和用于第二种昆虫的非交叉耐药性控制的三种蛋白质。 在优选的实施方式中，目标昆虫是FAW和ECB。

    Insect resistance management with combinations of Cry1Be and Cry1F proteins
    8.
    发明授权
    Insect resistance management with combinations of Cry1Be and Cry1F proteins 有权
    昆虫抗性管理与Cry1Be和Cry1F蛋白的组合

    公开(公告)号：US09556453B2

    公开(公告)日：2017-01-31

    申请号：US13516629

    申请日：2010-12-16

    申请人： Thomas Meade ,  Kenneth Narva ,  Nicholas P. Storer ,  Joel J. Sheets ,  Stephanie L. Burton ,  Aaron T. Woosley

    发明人： Thomas Meade ,  Kenneth Narva ,  Nicholas P. Storer ,  Joel J. Sheets ,  Stephanie L. Burton ,  Aaron T. Woosley

    IPC分类号： C12N15/82 ,  A01N63/02 ,  A01N65/00

    CPC分类号： C12N15/8286 ,  A01N63/02 ,  A01N65/00 ,  Y02A40/162 ,  A01N2300/00

    摘要： The subject invention relates in part to stacking Cry1Be toxins along with Cry1Fa toxins to prevent insects from developing resistance towards either toxin by itself. As discussed in more detail herein, the subject pair of proteins is a particularly advantageous combination, as no other pair of proteins is known to provide high levels of control and non-cross-resistant activity against both Spodoptera frugiperda (FAW) and Ostrinia nubilalis (ECB) insects. This dual, non-cross-resistant activity is also advantageous because it can reduce the number of proteins/genes needed to target these insects with multiple, non-cross-resistant proteins. This can reduce or eliminate the need for refuge acreage. Accordingly, the subject invention also relates generally to using four genes to provide three proteins for non-cross-resistant control of a first insect, and three proteins for non-cross-resistant control of a second insect. In preferred embodiments, the targeted insects are FAW and ECB.

    摘要翻译： 本发明部分涉及将Cry1Be毒素与Cry1Fa毒素一起堆叠以防止昆虫本身对毒素产生抗性。 如本文更详细讨论的那样，本发明的对象蛋白质是特别有利的组合，因为没有其他蛋白质对可以提供针对草地贪夜蛾（Spodoptera frugiperda）（FAW）和鸵鸟鸵鸟（Ostrinia nubilalis）的高水平的控制和非交叉抗性活性 ECB）昆虫。 这种双重非交叉抗性活性也是有利的，因为它可以减少用多种非交叉抗性蛋白质靶向这些昆虫所需的蛋白质/基因的数量。 这可以减少或消除避难所面积的需要。 因此，本发明还通常涉及使用四个基因提供用于第一昆虫的非交叉耐药性控制的三种蛋白质和用于第二种昆虫的非交叉耐药性控制的三种蛋白质。 在优选的实施方式中，目标昆虫是FAW和ECB。