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公开(公告)号:US20070054872A1
公开(公告)日:2007-03-08
申请号:US11466797
申请日:2006-08-24
申请人: Thomas Reppen , David Lewis
发明人: Thomas Reppen , David Lewis
CPC分类号: C12N15/85 , C12N2830/005 , C12N2830/008 , C12N2830/32 , C12N2840/102
摘要: Endogenous gene regulation mechanisms together with microRNAs expressed in many organisms can be used to provide regulated or conditional expression of transgenes by placing an appropriate sequence, a microRNA binding site, within the transcribed gene. This microRNA-dependent transcription regulation can be further regulated using microRNA inhibitors.
摘要翻译: 内源性基因调控机制与许多生物体中表达的微小RNA可以用于通过在转录的基因内放置适当的序列,即微小RNA结合位点来提供转基因的调节或条件表达。 这种microRNA依赖性转录调控可以使用微小RNA抑制剂进一步调控。
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公开(公告)号:US20060265771A1
公开(公告)日:2006-11-23
申请号:US11412154
申请日:2006-04-26
申请人: David Lewis , Thomas Reppen , Paula Roesch
发明人: David Lewis , Thomas Reppen , Paula Roesch
IPC分类号: A01K67/027 , C40B30/06 , C40B40/08
CPC分类号: C12N15/8509 , A01K2267/0393
摘要: In vivo endogenous microRNA (miRNA) activity can be observed over time using miRNA sensor plasmids capable of long term expression. Using reporter genes whose expression can be monitored without sacrificing the animal enables the investigator to follow changes in miRNA expression though developmental stages or in response to environmental factors or treatment regimens.
摘要翻译: 使用能够长期表达的miRNA传感器质粒,可以随时间观察体内内源性微小RNA(miRNA)活性。 使用可以在不牺牲动物的情况下监测其表达的报道基因使得研究者能够跟踪miRNA表达的变化,尽管通过发育阶段或响应于环境因素或治疗方案。
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