公开(公告)号：US20100330121A1

公开(公告)日：2010-12-30

申请号：US12845516

申请日：2010-07-28

申请人： Thomas W. Dubensky, JR. ,  John M. Polo ,  Barbara A. Belli ,  Sergey A. Dryga ,  Sondra Schlessinger ,  Ilya Frolov

发明人： Thomas W. Dubensky, JR. ,  John M. Polo ,  Barbara A. Belli ,  Sergey A. Dryga ,  Sondra Schlessinger ,  Ilya Frolov

IPC分类号： A61K39/12 ,  C12N15/63 ,  A61P37/04

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K48/00 ,  C12N7/00 ,  C12N15/79 ,  C12N15/86 ,  C12N2770/36121 ,  C12N2770/36122 ,  C12N2770/36143 ,  C12N2770/36162 ,  C12N2830/005 ,  C12N2830/006 ,  C12N2830/42 ,  C12N2830/48 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/44

摘要： Isolated nucleic acid molecules are disclosed, comprising an alphavirus nonstructural protein gene which, when operably incorporated into a recombinant alphavirus particle, eukaryotic layered vector initiation system, or RNA vector replicon, has a reduced level of vector-specific RNA synthesis, as compared to wild-type, and the same or greater level of proteins encoded by RNA transcribed from the viral junction region promoter, as compared to a wild-type recombinant alphavirus particle. Also disclosed are RNA vector replicons, alphavirus vector constructs, and eukaryotic layered vector initiation systems which contain the above-identified nucleic acid molecules.

摘要翻译： 公开了分离的核酸分子，其包含甲病毒非结构蛋白基因，当可操作地并入重组甲病毒颗粒中时，与野生型相比，真核分子载体启动系统或RNA载体复制子具有降低的载体特异性RNA合成水平 类型，以及与野生型重组甲病毒颗粒相比，从病毒连接区启动子转录的RNA编码的相同或更高水平的蛋白质。 还公开了RNA载体复制子，甲病毒载体构建体和含有上述核酸分子的真核分子载体启动系统。