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1.发明申请RECOMBINANT ALPHAVIRUS-BASED VECTORS WITH REDUCED INHIBITION OF CELLULAR MACRO-MOLECULAR SYNTHESIS 审中-公开基于重组腺病毒的载体，具有减少细胞宏观分子合成的抑制作用公开(公告)号：US20100330121A1
公开(公告)日：2010-12-30
申请号：US12845516
申请日：2010-07-28
申请人： Thomas W. Dubensky, JR. , John M. Polo , Barbara A. Belli , Sergey A. Dryga , Sondra Schlessinger , Ilya Frolov
发明人： Thomas W. Dubensky, JR. , John M. Polo , Barbara A. Belli , Sergey A. Dryga , Sondra Schlessinger , Ilya Frolov
CPC分类号： C07K14/005 , A61K39/00 , A61K48/00 , C12N7/00 , C12N15/79 , C12N15/86 , C12N2770/36121 , C12N2770/36122 , C12N2770/36143 , C12N2770/36162 , C12N2830/005 , C12N2830/006 , C12N2830/42 , C12N2830/48 , C12N2840/20 , C12N2840/203 , C12N2840/44
摘要： Isolated nucleic acid molecules are disclosed, comprising an alphavirus nonstructural protein gene which, when operably incorporated into a recombinant alphavirus particle, eukaryotic layered vector initiation system, or RNA vector replicon, has a reduced level of vector-specific RNA synthesis, as compared to wild-type, and the same or greater level of proteins encoded by RNA transcribed from the viral junction region promoter, as compared to a wild-type recombinant alphavirus particle. Also disclosed are RNA vector replicons, alphavirus vector constructs, and eukaryotic layered vector initiation systems which contain the above-identified nucleic acid molecules.
摘要翻译： 公开了分离的核酸分子，其包含甲病毒非结构蛋白基因，当可操作地并入重组甲病毒颗粒中时，与野生型相比，真核分子载体启动系统或RNA载体复制子具有降低的载体特异性RNA合成水平 类型，以及与野生型重组甲病毒颗粒相比，从病毒连接区启动子转录的RNA编码的相同或更高水平的蛋白质。 还公开了RNA载体复制子，甲病毒载体构建体和含有上述核酸分子的真核分子载体启动系统。
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公开(公告)号：US20100173412A1
公开(公告)日：2010-07-08
申请号：US12538494
申请日：2009-08-10
申请人： Thomas W. Dubensky, JR. , John M. Polo , Carlos E. Ibanez , Stephen M.W. Chang , Douglas J. Jolly , David A. Driver , Barbara A. Belli
发明人： Thomas W. Dubensky, JR. , John M. Polo , Carlos E. Ibanez , Stephen M.W. Chang , Douglas J. Jolly , David A. Driver , Barbara A. Belli
IPC分类号： C12N5/071
CPC分类号： C12N15/113 , A61K38/00 , A61K48/00 , A61K2039/5256 , C07K14/005 , C07K14/47 , C07K14/705 , C07K14/755 , C07K14/82 , C12N9/2402 , C12N15/1131 , C12N15/86 , C12N2310/111 , C12N2310/122 , C12N2710/10322 , C12N2710/16722 , C12N2730/10122 , C12N2740/15022 , C12N2740/16222 , C12N2770/36122 , C12N2830/002 , C12N2830/42 , C12N2830/60 , C12N2840/20 , C12N2840/203 , C12N2840/206 , C12N2840/44 , C12N2840/85 , C12Y302/01045 , Y02A50/388 , Y02A50/396 , Y02A50/466 , Y10S977/773 , Y10S977/804 , Y10S977/808 , Y10S977/918 , Y10S977/92 , Y10S977/924
摘要： The present disclosure provides compositions and methods for utilizing recombinant alphavirus vectors. Also disclosed are compositions and methods for making and utilizing eukaryotic layered vector initiation systems
摘要翻译： 本公开提供了利用重组甲病毒载体的组合物和方法。 还公开了用于制备和利用真核分层载体引发系统的组合物和方法
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3.发明申请公开(公告)号：US20080292655A1
公开(公告)日：2008-11-27
申请号：US12111305
申请日：2008-04-29
CPC分类号： C12N15/79
摘要： Expression cassettes are provided comprising a promoter operably linked to a nucleic acid molecule which, when transcribed in vivo, forms double-stranded RNA that induces the production of interferon. Expression cassettes also are provided comprising a promoter operably linked to a ribozyme or antisense nucleic acid molecule which, when transcribed in vivo, forms a ribozyme or antisense RNA molecule that stimulates an immune response. In addition, expression cassettes are provided comprising a promoter operably linked to a ribozyme or antisense nucleic acid molecule which, when transcribed in vivo, stimulates apoptosis. Finally, gene delivery vectors are provided which contain such expression cassettes, host cells containing the gene delivery vectors, and methods of utilizing the expression cassettes, gene delivery vectors, and host cells.
摘要翻译： 提供了表达盒，其包括可操作地连接到核酸分子的启动子，其在体内转录时形成诱导产生干扰素的双链RNA。 还提供了包含与核酶或反义核酸分子可操作地连接的启动子的表达盒，其在体内转录时形成刺激免疫应答的核酶或反义RNA分子。 此外，提供了包含可操作地连接到核酶或反义核酸分子的启动子的表达盒，其在体内转录时刺激细胞凋亡。 最后，提供含有这种表达盒的基因递送载体，含有基因递送载体的宿主细胞，以及利用表达盒，基因递送载体和宿主细胞的方法。
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4.发明申请Compositions and Methods for Generating an Immune Response Utilizing Alphavirus-Based Vector Systems 失效用于产生使用基于甲病毒的载体系统的免疫应答的组合物和方法公开(公告)号：US20100266631A1
公开(公告)日：2010-10-21
申请号：US12825722
申请日：2010-06-29
申请人： John Polo , Thomas W. Dubensky, JR. , Ilya Frolov , Jason P. Gardner , Gillis Otten , Susan Barnett , David A. Driver
发明人： John Polo , Thomas W. Dubensky, JR. , Ilya Frolov , Jason P. Gardner , Gillis Otten , Susan Barnett , David A. Driver
CPC分类号： A61K39/12 , A61K2039/5256 , A61K2039/545 , C07K14/005 , C12N7/00 , C12N2740/16134 , C12N2740/16234 , C12N2770/36121 , C12N2770/36122 , C12N2770/36143 , C12N2770/36145 , C12N2810/609
摘要： Compositions and methods are provided for Eukaryotic Layered Vector Initiation Systems and Alphavirus replicon particles for introducing heterologous sequences into cells for generating immune responses.
摘要翻译： 提供了用于真核分层载体启动系统和甲病毒复制子颗粒的组合物和方法，用于将异源序列引入细胞以产生免疫应答。
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公开(公告)号：US20120231526A1
公开(公告)日：2012-09-13
申请号：US13430920
申请日：2012-03-27
申请人： John M. Polo , Catherine Greer , Maria Calderon-Cacia , Daniel De La Vega, JR. , Thomas W. Dubensky, JR.
发明人： John M. Polo , Catherine Greer , Maria Calderon-Cacia , Daniel De La Vega, JR. , Thomas W. Dubensky, JR.
IPC分类号： C12N7/02
CPC分类号： C12N7/00 , A61K48/00 , C12N15/86 , C12N2770/36151 , C12Q1/6897 , C12Q1/701
摘要： Methods of production and purification for viruses and virus-derived vectors, including those related to alphaviruses, are disclosed. In one aspect, methods of purification that subject alphavirus replicon particle preparations to one or more steps of chromatographic purification, such as using an ion exchange resin, are provided. Also disclosed are methods of characterizing alphavirus replicon particles and utilizing these materials for vaccines and gene-based therapeutics
摘要翻译： 公开了病毒和病毒衍生载体的生产和纯化方法，包括与甲病毒相关的方法。 一方面，提供将甲病毒复制子颗粒制备物进行一步或多步色谱纯化的纯化方法，例如使用离子交换树脂。 还公开了表征甲病毒复制子颗粒并利用这些材料用于疫苗和基于基因的治疗剂的方法
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6.发明申请公开(公告)号：US20100055128A1
公开(公告)日：2010-03-04
申请号：US12212978
申请日：2008-09-18
申请人： John M. Polo , Catherine Greer , Maria Calderon-Cacia , Daniel De La Vega, JR. , Thomas W. Dubensky, JR.
发明人： John M. Polo , Catherine Greer , Maria Calderon-Cacia , Daniel De La Vega, JR. , Thomas W. Dubensky, JR.
CPC分类号： C12N7/00 , A61K48/00 , C12N15/86 , C12N2770/36151 , C12Q1/6897 , C12Q1/701
摘要： Methods of production and purification for viruses and virus-derived vectors, including those related to alphaviruses, are disclosed. in one aspect, methods of purification that subject alphavirus replicon particle preparations to one or more steps of chromatographic purification, such as using an ion exchange resin, are provided. Also disclosed are methods of characterizing alphavirus replicon particles and utilizing these materials for vaccines and gene-based therapeutics.
摘要翻译： 公开了病毒和病毒衍生载体的生产和纯化方法，包括与甲病毒相关的方法。 在一个方面，提供了使甲病毒复制子颗粒制备物进行一个或多个色谱纯化步骤（例如使用离子交换树脂）的纯化方法。 还公开了表征甲病毒复制子颗粒并利用这些材料用于疫苗和基于基因的治疗剂的方法。
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7.发明申请公开(公告)号：US20120039932A1
公开(公告)日：2012-02-16
申请号：US13277900
申请日：2011-10-20
IPC分类号： A61K39/00 , A61P37/04 , A61P35/00 , A61P31/16 , A61P31/20 , A61P31/22 , A61P31/14 , C12N15/867 , A61P31/18
CPC分类号： C12N15/86 , A61K39/0011 , A61K2039/5256 , C12N7/00 , C12N2740/15022 , C12N2740/15043 , C12N2770/36145 , Y02A50/466
摘要： Lentiviral vector particles comprising a Sindbis virus E2 glycoprotein variant and a lentiviral vector genome comprising a sequence of interest are provided. A lentiviral vector particle comprising: (a) an envelope comprising a Sindbis virus E2 glycoprotein variant; and (b) a lentiviral vector genome comprising a sequence of interest; wherein the E2 glycoprotein variant facilitates infection of dendritic cells by the lentiviral vector particle, and wherein the E2 glycoprotein variant has reduced binding to heparan sulfate compared to a reference sequence (HR strain).
摘要翻译： 提供包含辛德毕斯病毒E2糖蛋白变体和包含感兴趣序列的慢病毒载体基因组的慢病毒载体颗粒。 一种慢病毒载体颗粒，包括：（a）包含辛德毕斯病毒E2糖蛋白变体的包膜; 和（b）包含感兴趣序列的慢病毒载体基因组; 其中E2糖蛋白变体便于慢病毒载体颗粒感染树突状细胞，并且其中E2糖蛋白变体与参考序列（HR应变）相比具有减少的与硫酸乙酰肝素的结合。
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8.发明申请LISTERIA ATTENUATED FOR ENTRY INTO NON-PHAGOCYTIC CELLS, VACCINES COMPRISING THE LISTERIA, AND METHODS OF USE THEREOF 有权被引入非糖代谢细胞的肠梗阻，包含肠炎的疫苗及其使用方法公开(公告)号：US20110201092A1
公开(公告)日：2011-08-18
申请号：US12748586
申请日：2010-03-29
IPC分类号： C12N1/20
CPC分类号： A61K39/07 , A61K2039/5154 , A61K2039/522 , A61K2039/523
摘要： The present invention provides Listeria that are attenuated for entry into non-phagocytic cells as well as a variety of methods of inducing immune responses involving administering compositions comprising the attenuated Listeria. Some of the attenuated Listeria are mutant Listeria that comprise at least one mutation in a gene encoding an invasin, such as an internalin. Some of the attenuated Listeria are further attenuated for cell-to-cell spread. Pharmaceutical compositions and vaccines useful in the methods of the invention are further provided. Methods of making and improving vaccines are also provided.
摘要翻译： 本发明提供减毒以进入非吞噬细胞的李斯特菌以及诱导涉及施用包含减毒李斯特菌的组合物的免疫应答的多种方法。 一些减毒李斯特氏菌是突变型李斯特菌，其在编码侵入素的基因（例如内含蛋白）中包含至少一个突变。 一些减毒的李斯特菌进一步减弱细胞到细胞的扩散。 还提供了可用于本发明方法的药物组合物和疫苗。 还提供了制备和改进疫苗的方法。
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9.发明申请公开(公告)号：US20120328655A1
公开(公告)日：2012-12-27
申请号：US13441502
申请日：2012-04-06
IPC分类号： A61K39/00 , A61K39/12 , A61P37/04 , A61K39/002 , A61K39/245 , A61K39/39 , A61K39/02
CPC分类号： A61K39/245 , A61K39/0011 , A61K39/12 , A61K39/39 , A61K2039/53 , A61K2039/545 , A61K2039/55566 , A61K2039/55572 , A61K2039/70 , C12N2710/16634 , C12N2740/15034 , C12N2740/16043 , C12N2810/609
摘要： Compositions and methods are provided herein for improved dual immunization strategies that induce in a subject an immune response that includes a humoral immune response and cellular immune response, both CD4 and CD8 T lymphocyte immune responses, thereby providing a complete adaptive immune response to one or more antigens. The methods described are therefore useful for treating and/or preventing (i.e., reducing the likelihood or risk of occurrence) different diseases, disorders, and conditions such as cancers and infectious diseases for which induction of both a humoral immune response and cellular immune response is desired and beneficial.
摘要翻译： 本文提供的组合物和方法用于改善的双重免疫策略，其在受试者中诱导包括CD4和CD8 T淋巴细胞免疫应答的体液免疫应答和细胞免疫应答的免疫应答，从而为一种或多种 抗原。 因此，所描述的方法可用于治疗和/或预防（即，降低发生的可能性或风险）不同的疾病，病症和病症，例如诱导体液免疫应答和细胞免疫应答的癌症和感染性疾病 期望和有益。
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10.发明申请Methods for Enhancing Immunogen Specific Immune Responses by Vectored Vaccines 审中-公开通过检测疫苗增强免疫原特异性免疫反应的方法公开(公告)号：US20120263754A1
公开(公告)日：2012-10-18
申请号：US13397216
申请日：2012-02-15
CPC分类号： A61K39/0011 , A61K39/00 , A61K39/12 , A61K39/21 , A61K39/39 , A61K2039/53 , A61K2039/545 , A61K2039/55561 , A61K2039/55572 , C12N2740/15034 , C12N2740/15043 , C12N2760/10034 , C12N2770/36143
摘要： Provided herein are methods for inducing a specific immune response in a subject by administering to the subject an immunogenic composition comprising a recombinant expression vector, or a vector particle comprising the recombinant expression vector, which vector comprises a polynucleotide sequence that encodes an immunogen of interest. The methods further comprise administering an adjuvant composition either concurrently or sequentially with the immunogenic composition.
摘要翻译： 本文提供了通过向受试者施用包含重组表达载体的免疫原性组合物或包含重组表达载体的载体颗粒，所述载体包含编码感兴趣的免疫原的多核苷酸序列来诱导受试者中特异性免疫应答的方法。 所述方法还包括与免疫原性组合物同时或依次施用佐剂组合物。
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