公开(公告)号：US20120301433A1

公开(公告)日：2012-11-29

申请号：US13387888

申请日：2010-07-29

申请人： Timothy Kuan-Ta Lu ,  Susan Lindquist ,  Rajaraman Krishnan ,  James Collins ,  Charles W. O'Donnell ,  Bonnie Berger Leighton ,  Srinivas Devadas

发明人： Timothy Kuan-Ta Lu ,  Susan Lindquist ,  Rajaraman Krishnan ,  James Collins ,  Charles W. O'Donnell ,  Bonnie Berger Leighton ,  Srinivas Devadas

IPC分类号： C12N7/01 ,  C12S9/00 ,  A61K35/76

CPC分类号： C07K14/4711 ,  A61K35/76 ,  A61K38/00 ,  C07K2319/735 ,  C12N2795/10232 ,  C12N2795/10243 ,  C12N2795/14132 ,  C12N2795/14143 ,  Y02A50/475 ,  A61K2300/00

摘要： The present invention generally relates to engineered bacteriophages which express amyloid peptides for the modulation (e.g. increase or decrease) of protein aggregates and amyloid formation. In some embodiments, the engineered bacteriophages express anti-amyloid peptides for inhibiting protein aggregation and amyloid formation, which can be useful in the treatment and prevention of and bacterial infections and biofilms. In some embodiments, the engineered bacteriophages express amyloid peptides for promoting amyloid formation, which are useful for increasing amyloid formation such as promoting bacterial biofilms. Other aspects relate to methods to inhibit bacteria biofilms, and methods for the treatment of amyloid related disorders, e.g., Alzheimer's disease using an anti-amyloid peptide engineered bacteriophages. Other aspects of the invention relate to engineered bacteriophages to express the amyloid peptides on the bacteriophage surface and/or secrete the amyloid peptides, e.g., anti-amyloid peptides and pro-amyloid peptides, and uses thereof for modulation protein aggregates and amyloid formation.

摘要翻译： 本发明一般涉及表达淀粉样肽用于调节（例如增加或减少）蛋白质聚集体和淀粉样蛋白形成的工程化噬菌体。 在一些实施方案中，工程改造的噬菌体表达用于抑制蛋白质聚集和淀粉样蛋白形成的抗淀粉样肽，其可用于治疗和预防细菌感染和生物膜。 在一些实施方案中，工程改造的噬菌体表达用于促进淀粉样蛋白形成的淀粉样肽，其可用于增加淀粉样蛋白形成，例如促进细菌生物膜。 其它方面涉及抑制细菌生物膜的方法，以及使用抗淀粉样蛋白肽工程化噬菌体治疗淀粉样蛋白相关病症例如阿尔茨海默氏病的方法。 本发明的其它方面涉及在噬菌体表面上表达淀粉样肽和/或分泌淀粉样蛋白肽（例如抗淀粉样蛋白肽和前淀粉样肽）的工程化噬菌体及其用于调节蛋白质聚集体和淀粉样蛋白形成的用途。