公开(公告)号：US07045680B2

公开(公告)日：2006-05-16

申请号：US10268838

申请日：2002-10-11

申请人： Tohyama Kohichi ,  Jeon-Gu Kang ,  Hyo-Yeon Lee ,  Jeong-Il Kim ,  Pill-Soon Song

发明人： Tohyama Kohichi ,  Jeon-Gu Kang ,  Hyo-Yeon Lee ,  Jeong-Il Kim ,  Pill-Soon Song

IPC分类号： C12N15/82 ,  C12N15/90 ,  A01H5/00

CPC分类号： C12N15/8261 ,  C07K14/415 ,  Y02A40/146

摘要： The present invention provides transgenic zoysiagrass plant cells transformed with a modified oat phytochrome A gene (S598A) in which the serine-598 codon was replaced with an alanine codon, which, when expressed, significantly suppresses shade avoidance reactions, such as dwarfish internodes and petioles, short leaves, increased branching, and dark-green leaf color. Said transgenic zoysiagrass shows a robust appearance and is expected to show enhanced resistance to biotic and abiotic stresses. Also provided are a recombinant DNA construct comprising the S598A gene which, when expressed, induces drastic suppression of shade avoidance reactions, transgenic plants produced by this method, and propagating seeds obtained from said transgenic plants.

摘要翻译： 本发明提供用经修饰的燕麦植物色素A基因（S598A）转化的转基因植物细胞植物细胞，其中丝氨酸-598密码子被丙氨酸密码子替代，当表达时，其显着抑制避光反应，例如fish鱼节间和叶柄 ，叶短，分枝增多，深绿色叶色。 所述转基因动物学表现出强烈的外观，并且预期显示出对生物和非生物胁迫的增强的抗性。 还提供了包含S598A基因的重组DNA构建体，当其表达时，其诱导阴性避免反应的剧烈抑制，通过该方法产生的转基因植物和繁殖从所述转基因植物获得的种子。

公开(公告)号：US06646185B2

公开(公告)日：2003-11-11

申请号：US09988580

申请日：2001-11-20

申请人： Tohyama Kohichi ,  Chang-Hyu Bae ,  Jeong-Gu Kang ,  Pill-Soon Song ,  Chung-Mo Park ,  Hyo Yeon Lee

发明人： Tohyama Kohichi ,  Chang-Hyu Bae ,  Jeong-Gu Kang ,  Pill-Soon Song ,  Chung-Mo Park ,  Hyo Yeon Lee

IPC分类号： C12N1582

CPC分类号： C12N15/8205

摘要： The present invention provides an efficient genetic transformation system for the zoysiagrass plant (Zoysia japonica Steud.). Also provided are optimized media compositions and culture conditions for the zoysiagrass transformation. The reliable transformation system for zoysiagrass was developed by optimizing several factors that significantly affect calli growth and plant regeneration. Callus type and co-cultivation period absolutely influenced the transformation efficiency. Concentrations of 2,4-D, CaCl2 and acetosyringone were also critical factors. The best result was achieved when type 3 calli were co-cultivated on a 2,4-D-free co-cultivation medium for 6 days. Removal of calcium and addition of 50 mg/liter acetosyringone during co-cultivation drastically enhanced the efficiency. The invention also provides a bialaphos-resistant zoysiagrass plant, which can be used in golf courses and athletic fields to save the maintenance cost.

公开(公告)号：US06489166B1

公开(公告)日：2002-12-03

申请号：US09915294

申请日：2001-07-27

申请人： Jeong-Gu Kang ,  Pill-Soon Song ,  Chung-Mo Park ,  Tohyama Kohichi ,  Hyo Yeon Lee

发明人： Jeong-Gu Kang ,  Pill-Soon Song ,  Chung-Mo Park ,  Tohyama Kohichi ,  Hyo Yeon Lee

IPC分类号： C12N500

CPC分类号： A01H4/005 ,  A01H4/008

摘要： Fully differentiated higher plants may be regenerated from undifferentiated plant tissues, such as callus, derived from mature seeds, immature embryos, stems, roots, or leaves. The present invention provides a plant regeneration method from mature seed-derived calli. The regeneration procedure may include the induction of calli on the MS media containing 2 mg/L 2,4-dichlorophenoxyacetic acid and 0.2 mg/L 6-benzylaminopurine, 4 mg/L thiamine-HCl, and 100 mg/L &agr;-ketoglutaric acid. The plant regeneration efficiency is the highest when calli are grown on the MS media supplemented with 3% maltose and 1 mg/L 6-benzylaminopurine or 1 mg/L thidiazuron (TDZ). The invention can be utilized to introduce a gene or genes of agronomically important values into the zoysiagrass or possibly closely related turfgrass species.

摘要翻译： 完全分化的高等植物可以从未分化的植物组织再生，例如来自成熟种子，未成熟胚，茎，根或叶的愈伤组织。 本发明提供来自成熟种子源愈伤组织的植物再生方法。 再生程序可以包括在含有2mg / L 2,4-二氯苯氧基乙酸和0.2mg / L 6-苄基氨基嘌呤，4mg / L硫胺素-HCl和100mg / Lα-酮戊二酸的MS培养基上诱导愈伤组织 。 当愈伤组织在补充有3％麦芽糖和1mg / L 6-苄基氨基嘌呤或1mg / L噻替隆（TDZ）的MS培养基上生长时，植物再生效率最高。 本发明可用于将具有农业重要价值的基因或基因引入动物学或可能紧密相关的草坪草种类。

公开(公告)号：US07285652B2

公开(公告)日：2007-10-23

申请号：US11129459

申请日：2005-05-16

申请人： Jeong-Il Kim ,  Yun-Jeong Han ,  Pill-Soon Song

发明人： Jeong-Il Kim ,  Yun-Jeong Han ,  Pill-Soon Song

IPC分类号： C07H21/04

CPC分类号： C12N15/8261 ,  C07K14/415 ,  Y02A40/146

摘要： The present invention includes modified phytochrome A (PHYA) nucleic acid molecules in which Pr absorption spectra have been shifted to longer wavelength (i.e. bathochromism or red-shift). The plants with the bathochromic phytochromes are expected to respond to canopy and shade conditions for growth and development with greater efficiency than the plants with wild-type phytochrome (i.e. suppression of shade avoidance reactions in plants). Since the shade avoidance reactions in plants induce a rapid and dramatic increase in the extension growth of stems and petioles at the expense of leaf growth, storage organ production, and reproductive development, it causes significant losses of crop yields. Thus, the bathochromic phytochromes that utilize the shade light efficiently would suppress the shade avoidance reactions in plants, giving plants the tolerance to shade. In this invention, several bathochromic phytochromes were generated by site-directed mutagenesis in the region of bilin lyase domain in plant PHYA, and their ability to suppress the shade avoidance reactions were examined by transforming the bathochromic phytochromes into PHYA deficient Arabidopsis thaliana (ecotype col-0). The transgenic plants with the bathochromic phytochromes showed significantly increased shade tolerance compared to wild-type plants and transgenic plants with wild-type phytochromes. Therefore, the present invention can be utilized to suppress plants' shade avoidance that is one of major causes to induce crop-yield losses, and ultimately to generate shade tolerant plants with higher yields. The invention also includes plants having at least one cell expressing the modified PHYA, vectors comprising at least one portion of the modified PHYA nucleic acids, and methods using such vectors for producing plants with shade tolerance.

摘要翻译： 本发明包括修饰的植物色素A（PHYA）核酸分子，其中Pr吸收光谱已经转移到更长的波长（即，红移或红移）。 具有红色色素植物色素的植物预期对具有野生型植物色素的植物（即抑制植物中的树荫回避反应）的效率比用于生长和发育的冠层和阴凉条件更有效。 由于植物中的避光反应以叶片生长，储存器官生产和繁殖发育为代价诱导茎和叶柄的延伸生长快速和显着地增加，导致作物产量的显着损失。 因此，有效利用遮光效果的红色植物色素可以抑制植物的避光反应，使植物具有遮荫性。 在本发明中，通过在植物PHYA中的胆固醇裂解酶结构域中的定点诱变产生了几种红色植物色素，并且通过将红色染色体变成PHYA缺陷拟南芥（生态型col- 0）。 具有红移植物色素的转基因植物与野生型植物和具有野生型植物色素的转基因植物相比，显示明显增加的遮荫耐受性。 因此，本发明可以用于抑制作物诱导作物产量损失的主要原因之一的植物避光，最终产生具有较高产量的耐荫植物。 本发明还包括具有至少一个表达经修饰的PHYA的细胞的植物，包含至少一部分修饰的PHYA核酸的载体，以及使用这种载体产生具有阴性耐受性的植物的方法。

公开(公告)号：US20060260009A1

公开(公告)日：2006-11-16

申请号：US11129459

申请日：2005-05-16

申请人： Jeong-Il Kim ,  Yun-Jeong Han ,  Pill-Soon Song

发明人： Jeong-Il Kim ,  Yun-Jeong Han ,  Pill-Soon Song

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/82

CPC分类号： C12N15/8261 ,  C07K14/415 ,  Y02A40/146

摘要： The present invention includes modified phytochrome A (PHYA) nucleic acid molecules in which Pr absorption spectra have been shifted to longer wavelength (i.e. bathochromism or red-shift). The plants with the bathochromic phytochromes are expected to respond to canopy and shade conditions for growth and development with greater efficiency than the plants with wild-type phytochrome (i.e. suppression of shade avoidance reactions in plants). Since the shade avoidance reactions in plants induce a rapid and dramatic increase in the extension growth of stems and petioles at the expense of leaf growth, storage organ production, and reproductive development, it causes significant losses of crop yields. Thus, the bathochromic phytochromes that utilize the shade light efficiently would suppress the shade avoidance reactions in plants, giving plants the tolerance to shade. In this invention, several bathochromic phytochromes were generated by site-directed mutagenesis in the region of bilin lyase domain in plant PHYA, and their ability to suppress the shade avoidance reactions were examined by transforming the bathochromic phytochromes into PHYA deficient Arabidopsis thaliana (ecotype col-0). The transgenic plants with the bathochromic phytochromes showed significantly increased shade tolerance compared to wild-type plants and transgenic plants with wild-type phytochromes. Therefore, the present invention can be utilized to suppress plants' shade avoidance that is one of major causes to induce crop-yield losses, and ultimately to generate shade tolerant plants with higher yields. The invention also includes plants having at least one cell expressing the modified PHYA, vectors comprising at least one portion of the modified PHYA nucleic acids, and methods using such vectors for producing plants with shade tolerance.

摘要翻译： 本发明包括修饰的植物色素A（PHYA）核酸分子，其中Pr吸收光谱已经转移到更长的波长（即，红移或红移）。 具有红色色素植物色素的植物预期对具有野生型植物色素的植物（即抑制植物中的树荫回避反应）的效率比用于生长和发育的冠层和阴凉条件更有效。 由于植物中的避光反应以叶片生长，储存器官生产和繁殖发育为代价诱导茎和叶柄的延伸生长快速和显着地增加，导致作物产量的显着损失。 因此，有效利用遮光效果的红色植物色素可以抑制植物的避光反应，使植物具有遮荫性。 在本发明中，通过在植物PHYA中的胆固醇裂解酶结构域中的定点诱变产生了几种红色植物色素，并且通过将红色染色体变成PHYA缺陷拟南芥（生态型col- 0）。 具有红移植物色素的转基因植物与野生型植物和具有野生型植物色素的转基因植物相比，显示明显增加的遮荫耐受性。 因此，本发明可以用于抑制作物诱导作物产量损失的主要原因之一的植物避光，最终产生具有较高产量的耐荫植物。 本发明还包括具有至少一个表达经修饰的PHYA的细胞的植物，包含至少一部分修饰的PHYA核酸的载体，以及使用这种载体产生具有阴性耐受性的植物的方法。

公开(公告)号：US07192775B1

公开(公告)日：2007-03-20

申请号：US11249345

申请日：2005-10-14

申请人： Young Soon Kim ,  Ae Ran Park ,  Moon Kyung Ko ,  Jae Bok Yoon ,  Hyo Guen Park ,  Pill-Soon Song

发明人： Young Soon Kim ,  Ae Ran Park ,  Moon Kyung Ko ,  Jae Bok Yoon ,  Hyo Guen Park ,  Pill-Soon Song

IPC分类号： C12N15/82 ,  C12N5/10 ,  C12N15/74 ,  C07H21/04

CPC分类号： C07K14/415 ,  C12N15/8282

摘要： The invention relates to a gDNA and corresponding cDNA sequence comprising pathogenesis related protein-10 (CbPR-10) gene in Capsicum baccatum. The polypeptide encoded by the DNA sequence has ribonuclease activity which makes the enzyme highly effective in inhibiting the growth of fungal pathogen. The use of the DNA sequence disclosed herein contains the gDNA, cDNA, protein and a genetic construct for the production of transgenic plants, especially genetically transformed pepper plants, with an enhanced resistance against fungal pathogens.

摘要翻译： 本发明涉及一种在辣椒中含有发病相关蛋白-10（CbPR-10）基因的gDNA和相应的cDNA序列。 由DNA序列编码的多肽具有核糖核酸酶活性，这使酶在抑制真菌病原体的生长方面非常有效。 本文公开的DNA序列的使用包含gDNA，cDNA，蛋白质和用于产生转基因植物，特别是经遗传转化的胡椒植物的遗传构建体，其对真菌病原体具有增强的抗性。

公开(公告)号：US07164013B2

公开(公告)日：2007-01-16

申请号：US10641100

申请日：2003-08-15

申请人： Moon-Soo Soh ,  Pill-Soon Song ,  Ki-Young Yang

发明人： Moon-Soo Soh ,  Pill-Soon Song ,  Ki-Young Yang

IPC分类号： C12N15/29 ,  C12N15/52

CPC分类号： C12N15/8237

摘要： This invention is about the functionally hyperactive light signal related molecule, HFR1-ΔN105, of which the nucleic acids that encode N-terminal 105 amino acid residues were deleted. HFR1 as a bHLH transcription factor functions in a subset of phytochrome A signaling cascade and it was reported to be regulated negatively by COP1. Experiments with a HFR1-ΔN105 overexpressing plant revealed that the deletion of N-terminal amino acids makes the HFR1 more active in photomorphogenic development such as germination and de-etiolation. In addition, the transgenic plants showed hypersensitive photo-responses in the inhibition of hypocotyl elongation, dependently on another positive element of light signaling, a bZIP protein, HY5. The end-of-day far-red light response and petiole elongation were suppressed in the HFR1-ΔN105 overexpressing plants. These results suggest that N-terminal region of HFR1 negatively regulate HFR1 function and that HFR1-ΔN105 is hyperactive.

摘要翻译： 本发明涉及功能性超活动光信号相关分子HFR1-DeltaN105，其中编码N末端105个氨基酸残基的核酸被缺失。 HFR1作为bHLH转录因子在植物色素A信号级联的一个子集中起作用，据报道COP1负调节。 HFR1-DeltaN105过表达植物的实验显示，N末端氨基酸的缺失使得HFR1在光形态发生如萌发和脱乙酰化中更具活性。 此外，转基因植物在抑制下胚轴延伸方面表现出过敏光响应，依赖于光信号传导的另一阳性元件bZIP蛋白HY5。 在HFR1-DeltaN105过表达植物中，终末的远红光反应和叶柄伸长被抑制。 这些结果表明，HFR1的N末端区域负调节HFR1功能，HFR1-DeltaN105是多活性的。

公开(公告)号：US20070136897A1

公开(公告)日：2007-06-14

申请号：US11247236

申请日：2005-10-12

申请人： Jeong-II Kim ,  Yun-Jeong Han ,  Seong-Hee Kim ,  Pill-Soon Song

发明人： Jeong-II Kim ,  Yun-Jeong Han ,  Seong-Hee Kim ,  Pill-Soon Song

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N5/04 ,  C12N15/82

CPC分类号： C12N9/1229 ,  C12N15/8261 ,  C12N15/8271 ,  Y02A40/146

摘要： The present invention includes modified Arabidopsis Nucleoside Di-Phosphate Kinase 2 (NDPK2) nucleic acid molecules whose enzymatic activity have been increased (i.e. hyperactive). NDPKs are ubiquitous housekeeping enzymes that catalyze the transfer of γ-phosphoryl group from a nucleoside triphosphate (NTP) to a nucleoside diphosphate (NDP), and also multifunctional proteins that regulate a variety of eukaryotic cellular activities, including cell proliferation, development, and differentiation. In plants, NDPKs are reported to play a key role in the signaling of both stress and light. Among three NDPKs (NDPK1, NDPK2, NDPK3) in a model plant, Arabidopsis thaliana, NDPK2 was reported as a positive signal transducer of phytochrome-mediated plant light signaling and to regulate cellular redox state, which enhances multiple stress tolerance in transgenic plants. Thus, the plants with the hyperactive NDPK2 are expected to possess higher efficiency of light utilization and enhanced tolerance to various environmental stresses such as cold, salt, and oxidative stresses. Since abiotic stress is one of the most important factors to limit the productivity of many crops, the hyperactive NDPK2 can be used for the development of high-yielding multiple stress tolerant plants with higher efficiency of light utilization. In this invention, several hyperactive NDPK2 were generated by C-terminal deletion and site-directed mutagenesis. Therefore, the present invention can be utilized to develop multiple stress tolerant and efficiently light-utilizing plants, which can eventually increase crop yields. The invention also includes plants having at least one cell expressing the modified NDPK2, vectors comprising at least one portion of the modified NDPK2 nucleic acids, and methods using such vectors for producing plants with enhanced light sensitivity and stress tolerance.

公开(公告)号：US5346083A

公开(公告)日：1994-09-13

申请号：US173280

申请日：1993-12-27

申请人： Pill-Soon Song ,  William R. Parker, Jr.

发明人： Pill-Soon Song ,  William R. Parker, Jr.

IPC分类号： B65D1/02 ,  B65D39/08 ,  B65D41/04 ,  B65D45/06 ,  B65D53/00

CPC分类号： B65D41/0414 ,  B65D1/023 ,  B65D39/08 ,  B65D45/06

摘要： To permit a container and a cap to remate with each other during resealing without fouling by solid debris such as small particles without exceptional preparations to withstand the deteriorating effects of corrosive materials and solvents and to consistently and quickly form a gas-tight seal, the container has a flat angled surface and the cap has cooperating edge that engages the flat surface in a closed line when the cap is fully inserted with a solid closed surface within the line, the closed line having a width perpendicular to its length within a range of 0.5 millimeters to 1.5 millimeters. The cap is closed by screw threads or the like that force the cooperating surfaces together with a pressure of at least 100 psi at an angle of between 75 degrees and 105 degrees.

摘要翻译： 为了允许容器和盖子在重新密封期间彼此重新凝结，而不会由诸如小颗粒的固体碎屑污染而没有特殊的制剂以抵抗腐蚀性材料和溶剂的劣化作用并且始终如一地形成气密密封，容器 具有平坦的倾斜表面，并且当盖完全插入线内的实心封闭表面时，盖具有与封闭线中的平坦表面接合的配合边缘，该闭合线具有垂直于其长度的宽度在0.5的范围内 毫米至1.5毫米。 帽被螺纹或类似物封闭，螺纹等将压配合表面以至少100psi的压力以75度和105度之间的角度相加。

公开(公告)号：US06946294B2

公开(公告)日：2005-09-20

申请号：US10345599

申请日：2003-01-16

申请人： Jeong-Gu Kang ,  Ju Yun ,  Pill-Soon Song ,  Chung-Mo Park

发明人： Jeong-Gu Kang ,  Ju Yun ,  Pill-Soon Song ,  Chung-Mo Park

IPC分类号： C12N5/06 ,  C07K14/415 ,  C12N15/82 ,  C12N5/10

CPC分类号： C12N15/8261 ,  C07K14/415 ,  Y02A40/146

摘要： The present invention provides transgenic higher plants and transgenic plant cells thereof which have been transformed with a plant translationally controlled tumor protein (TCTP) gene to improve growth rate in a growth stage-dependent manner, especially in the earlier vegetative growth stage from seedlings to young plants. Also, the invention provides methods for engineering higher plants so that they can be more efficiently transformed with other transforming vector constructs. Further, provided are TCTP expression vectors which contain an uninterrupted coding region for the TCTP protein used to generate transgenic plants and cells thereof.

摘要翻译： 本发明提供已经用植物翻译控制的肿瘤蛋白（TCTP）基因转化的转基因高等植物和转基因植物细胞，以改善生长阶段依赖性的生长速率，特别是在从幼苗到幼年的早期营养生长阶段 植物。 此外，本发明提供了用于工程化高等植物以使其可以用其他转化载体构建体更有效地转化的方法。 此外，提供了TCTP表达载体，其含有用于产生转基因植物及其细胞的TCTP蛋白的不间断编码区。