公开(公告)号：US07728194B2

公开(公告)日：2010-06-01

申请号：US10555824

申请日：2004-05-07

申请人： Byung-Dong Kim ,  Dong-Hwan Kim ,  Jeong-Gu Kang

发明人： Byung-Dong Kim ,  Dong-Hwan Kim ,  Jeong-Gu Kang

IPC分类号： C12N15/29 ,  C12N15/62 ,  C12N15/82 ,  A01H1/02 ,  A01H5/00

CPC分类号： C12Q1/6888 ,  A01H1/02 ,  C07K14/415 ,  C12N15/8289 ,  C12Q2600/158

摘要： The present invention relates to a DNA fragment specific to a cytoplasmic male sterile pepper comprising a polynucleotide of SEQ ID NO: 1, a candidate polynucleotide (named orf456) associated with cytoplasmic male sterile pepper consisting of a 223rd to 678th nucleic acid of SEQ ID NO:1, and a polynucleotide of SEQ ID NO: 2. The DNA fragment specific to cytoplasmic male sterile pepper comprising a polynucleotide of SEQ ID NOs: 1 or 2 can be used for identifying cytoplasmic type between male sterile and male fertile pepper by the PCR method. In addition, hybrid pepper breeders/seed companies could detect impurities of the maintainer line within the CMS line, and by ensuring purity of the CMS line, a major source of contamination of the hybrid seeds is removed leading to obvious benefits for the seed industry and farmers.

摘要翻译： 本发明涉及特异于细胞质雄性不育胡椒的DNA片段，其包含SEQ ID NO：1的多核苷酸，与由SEQ ID NO：223的第223至第678位核酸组成的细胞质雄性不育胡椒相关的候选多核苷酸（命名为orf456） ：1和SEQ ID NO：2的多核苷酸。包含SEQ ID NO：1或2的多核苷酸的细胞质雄性不育胡椒特异性的DNA片段可用于通过PCR鉴定雄性不育和雄性可育辣椒之间的细胞质型 方法。 此外，杂交辣椒种子/种子公司可以检测CMS系统内维护线的杂质，并通过确保CMS系的纯度，去除混合种子的主要污染源，从而为种业提供明显的益处， 农民

公开(公告)号：US20070180582A1

公开(公告)日：2007-08-02

申请号：US10555824

申请日：2004-05-07

申请人： Byung-Dong Kim ,  Dong-Hwan Kim ,  Jeong-Gu Kang

发明人： Byung-Dong Kim ,  Dong-Hwan Kim ,  Jeong-Gu Kang

IPC分类号： A01H5/00 ,  C12Q1/68 ,  C12N15/82 ,  C12N5/04

CPC分类号： C12Q1/6888 ,  A01H1/02 ,  C07K14/415 ,  C12N15/8289 ,  C12Q2600/158

摘要： The present invention relates to a DNA fragment specific to a cytoplasmic male sterile pepper comprising a polynucleotide of SEQ ID NO: 1, a candidate polynucleotide (named orf456) associated with cytoplasmic male sterile pepper consisting of a 223rd to 678th nucleic acid of SEQ ID NO:1, and a polynucleotide of SEQ ID NO: 2. The DNA fragment specific to cytoplasmic male sterile pepper comprising a polynucleotide of SEQ ID NOs: 1 or 2 can be used for identifying cytoplasmic type between male sterile and male fertile pepper by the PCR method. In addition, hybrid pepper breeders/seed companies could detect impurities of the maintainer line within the CMS line, and by ensuring purity of the CMS line, a major source of contamination of the hybrid seeds is removed leading to obvious benefits for the seed industry and farmers.

摘要翻译： 本发明涉及特异于细胞质雄性不育胡椒的DNA片段，其包含SEQ ID NO：1的多核苷酸，与由SEQ ID NO：223的第223至第678位核酸组成的细胞质雄性不育胡椒相关的候选多核苷酸（命名为orf456） ：1和SEQ ID NO：2的多核苷酸。包含SEQ ID NO：1或2的多核苷酸的细胞质雄性不育胡椒特异性的DNA片段可用于通过PCR鉴定雄性不育和雄性可育辣椒之间的细胞质型 方法。 此外，杂交辣椒种子/种子公司可以检测CMS系统内维护线的杂质，并通过确保CMS系的纯度，去除混合种子的主要污染源，从而为种业提供明显的益处， 农民

公开(公告)号：US06946294B2

公开(公告)日：2005-09-20

申请号：US10345599

申请日：2003-01-16

申请人： Jeong-Gu Kang ,  Ju Yun ,  Pill-Soon Song ,  Chung-Mo Park

发明人： Jeong-Gu Kang ,  Ju Yun ,  Pill-Soon Song ,  Chung-Mo Park

IPC分类号： C12N5/06 ,  C07K14/415 ,  C12N15/82 ,  C12N5/10

CPC分类号： C12N15/8261 ,  C07K14/415 ,  Y02A40/146

摘要： The present invention provides transgenic higher plants and transgenic plant cells thereof which have been transformed with a plant translationally controlled tumor protein (TCTP) gene to improve growth rate in a growth stage-dependent manner, especially in the earlier vegetative growth stage from seedlings to young plants. Also, the invention provides methods for engineering higher plants so that they can be more efficiently transformed with other transforming vector constructs. Further, provided are TCTP expression vectors which contain an uninterrupted coding region for the TCTP protein used to generate transgenic plants and cells thereof.

摘要翻译： 本发明提供已经用植物翻译控制的肿瘤蛋白（TCTP）基因转化的转基因高等植物和转基因植物细胞，以改善生长阶段依赖性的生长速率，特别是在从幼苗到幼年的早期营养生长阶段 植物。 此外，本发明提供了用于工程化高等植物以使其可以用其他转化载体构建体更有效地转化的方法。 此外，提供了TCTP表达载体，其含有用于产生转基因植物及其细胞的TCTP蛋白的不间断编码区。

公开(公告)号：US06518484B2

公开(公告)日：2003-02-11

申请号：US09815622

申请日：2001-03-23

申请人： Jeong-Gu Kang ,  Ju Yun ,  Kyung-Sook Chung ,  Pill-Soon Song ,  Chung-Mo Park

发明人： Jeong-Gu Kang ,  Ju Yun ,  Kyung-Sook Chung ,  Pill-Soon Song ,  Chung-Mo Park

IPC分类号： A01H100

CPC分类号： C07K14/415 ,  C12N15/8216

摘要： The present invention provides a nucleic acid molecule isolated from the 5′ untranscribed region of a translationally controlled tumor protein (TCTP) gene from Arabidopsis. The nucleic acid molecule, referred to as TCTP promoter in the present invention, directs the high-level expression of a reporter gene that is properly placed under its control. The TCTP promoter initiates and regulates the gene expression to a level that is comparable to those by the currently available promoters in plant genetic engineering. A minimum of 300-bp region is enough for fill regulatory activity. The TCTP promoter is functional in all plant tissues tested with the highest activity in the meristem tissue of root tip. The present invention can be utilized to express useful genes to a high level in agronomically important plants.

公开(公告)号：US06646185B2

公开(公告)日：2003-11-11

申请号：US09988580

申请日：2001-11-20

申请人： Tohyama Kohichi ,  Chang-Hyu Bae ,  Jeong-Gu Kang ,  Pill-Soon Song ,  Chung-Mo Park ,  Hyo Yeon Lee

发明人： Tohyama Kohichi ,  Chang-Hyu Bae ,  Jeong-Gu Kang ,  Pill-Soon Song ,  Chung-Mo Park ,  Hyo Yeon Lee

IPC分类号： C12N1582

CPC分类号： C12N15/8205

摘要： The present invention provides an efficient genetic transformation system for the zoysiagrass plant (Zoysia japonica Steud.). Also provided are optimized media compositions and culture conditions for the zoysiagrass transformation. The reliable transformation system for zoysiagrass was developed by optimizing several factors that significantly affect calli growth and plant regeneration. Callus type and co-cultivation period absolutely influenced the transformation efficiency. Concentrations of 2,4-D, CaCl2 and acetosyringone were also critical factors. The best result was achieved when type 3 calli were co-cultivated on a 2,4-D-free co-cultivation medium for 6 days. Removal of calcium and addition of 50 mg/liter acetosyringone during co-cultivation drastically enhanced the efficiency. The invention also provides a bialaphos-resistant zoysiagrass plant, which can be used in golf courses and athletic fields to save the maintenance cost.

公开(公告)号：US06586252B2

公开(公告)日：2003-07-01

申请号：US09916338

申请日：2001-07-30

申请人： Jeong-Gu Kang ,  Pill-Soon Song ,  Chung-Mo Park

发明人： Jeong-Gu Kang ,  Pill-Soon Song ,  Chung-Mo Park

IPC分类号： C12N1529

CPC分类号： C12N9/16 ,  C12N15/827

摘要： This invention relates to a nucleic acid molecule encoding the catalytic subunit of a protein phosphatase (PP2AC-JD) that belongs to the PP2A family. The PP2AC-JD interacts with the phytochrome A, a primary photoreceptor in the light signal transduction in plants, in the photoperiodic control of flowering. The present invention also provides the methods and processes for generating transgenic higher plants transformed with said nucleic acid molecule to engineer flowing time of economically important crop plants.

摘要翻译： 本发明涉及编码属于PP2A家族的蛋白质磷酸酶（PP2AC-JD）的催化亚基的核酸分子。 PP2AC-JD与植物色素A（植物光信号转导中的初级感光体）在开花的光周期控制中相互作用。 本发明还提供用于产生用所述核酸分子转化的转基因高等植物以设计经济上重要的作物植物的流动时间的方法和方法。

公开(公告)号：US06545202B2

公开(公告)日：2003-04-08

申请号：US09737300

申请日：2000-12-15

申请人： Jeong-Gu Kang ,  Ju Yun ,  Pill-Soon Song ,  Chung-Mo Park

发明人： Jeong-Gu Kang ,  Ju Yun ,  Pill-Soon Song ,  Chung-Mo Park

IPC分类号： A01H1100

CPC分类号： C12N15/8261 ,  C07K14/415 ,  Y02A40/146

摘要： The present invention provides transgenic higher plants and transgenic plant cells thereof which have been transformed with a plant translationally controlled tumor protein (TCTP) gene to improve growth rate in a growth stage-dependent manner, especially in the earlier vegetative growth stage from seedlings to young plants. Also, the invention provides methods for engineering higher plants so that they can be more efficiently transformed with other transforming vector constructs. Further, provided are TCTP expression vectors which contain an uninterrupted coding region for the TCTP protein used to generate transgenic plants and cells thereof.

公开(公告)号：US06489166B1

公开(公告)日：2002-12-03

申请号：US09915294

申请日：2001-07-27

申请人： Jeong-Gu Kang ,  Pill-Soon Song ,  Chung-Mo Park ,  Tohyama Kohichi ,  Hyo Yeon Lee

发明人： Jeong-Gu Kang ,  Pill-Soon Song ,  Chung-Mo Park ,  Tohyama Kohichi ,  Hyo Yeon Lee

IPC分类号： C12N500

CPC分类号： A01H4/005 ,  A01H4/008

摘要： Fully differentiated higher plants may be regenerated from undifferentiated plant tissues, such as callus, derived from mature seeds, immature embryos, stems, roots, or leaves. The present invention provides a plant regeneration method from mature seed-derived calli. The regeneration procedure may include the induction of calli on the MS media containing 2 mg/L 2,4-dichlorophenoxyacetic acid and 0.2 mg/L 6-benzylaminopurine, 4 mg/L thiamine-HCl, and 100 mg/L &agr;-ketoglutaric acid. The plant regeneration efficiency is the highest when calli are grown on the MS media supplemented with 3% maltose and 1 mg/L 6-benzylaminopurine or 1 mg/L thidiazuron (TDZ). The invention can be utilized to introduce a gene or genes of agronomically important values into the zoysiagrass or possibly closely related turfgrass species.

摘要翻译： 完全分化的高等植物可以从未分化的植物组织再生，例如来自成熟种子，未成熟胚，茎，根或叶的愈伤组织。 本发明提供来自成熟种子源愈伤组织的植物再生方法。 再生程序可以包括在含有2mg / L 2,4-二氯苯氧基乙酸和0.2mg / L 6-苄基氨基嘌呤，4mg / L硫胺素-HCl和100mg / Lα-酮戊二酸的MS培养基上诱导愈伤组织 。 当愈伤组织在补充有3％麦芽糖和1mg / L 6-苄基氨基嘌呤或1mg / L噻替隆（TDZ）的MS培养基上生长时，植物再生效率最高。 本发明可用于将具有农业重要价值的基因或基因引入动物学或可能紧密相关的草坪草种类。

公开(公告)号：US06605469B1

公开(公告)日：2003-08-12

申请号：US09689783

申请日：2000-10-13

申请人： Jeong-Gu Kang ,  Chung-Mo Park

发明人： Jeong-Gu Kang ,  Chung-Mo Park

IPC分类号： C12N121

CPC分类号： C12N9/0077 ,  C12N15/8298

摘要： The present invention provides a nucleic acid molecule encoding a dark-inducible cytochrome P450 hydroxylase that catalyzes the brassinosteroid biosynthesis through C-2 hydroxylations in plants. The invention also describes the methods and processes for generating expression cassettes and plasmids and for the use of these expression cassettes and plasmids to synthesize the cytochrome P450 hydroxylase or biologically active fragments of such an enzyme. The invention can be utilized to improve or decrease the stem growth of transgenic plants containing the nucleic acid molecule so that they exhibit improved growth rate and resistance to environmental stress and to identify other proteins involved in the brassinosteroid biosynthesis and in the plant growth regulation.

摘要翻译： 本发明提供编码暗诱导细胞色素P450羟化酶的核酸分子，其通过植物中的C-2羟基化催化油菜素内固醇生物合成。 本发明还描述了产生表达盒和质粒的方法和方法，并且使用这些表达盒和质粒来合成这种酶的细胞色素P450羟化酶或生物活性片段。 本发明可以用于改善或减少含有核酸分子的转基因植物的茎生长，使得它们表现出改善的生长速率和对环境胁迫的抗性，并且鉴定涉及油菜素内酯生物合成和植物生长调节的其它蛋白质。