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公开(公告)号：US07858370B2
公开(公告)日：2010-12-28
申请号：US11856978
申请日：2007-09-18
申请人： Toni Armstrong , Kenneth Barton , Laura J. Crow , Larry A. Gilbertson , Shihshieh Huang , Yong Huang , Brian J. Martinell , Michael W. Petersen , Kenneth J. Piller , Qi Wang , Xudong Ye
发明人： Toni Armstrong , Kenneth Barton , Laura J. Crow , Larry A. Gilbertson , Shihshieh Huang , Yong Huang , Brian J. Martinell , Michael W. Petersen , Kenneth J. Piller , Qi Wang , Xudong Ye
IPC分类号： C12N15/82
CPC分类号： C12N15/8205 , C12N15/8209 , C12N15/8263 , C12N15/8265 , C12N15/8289
摘要： The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independent transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.
摘要翻译： 提供的组合物和方法增强了在不同物理和遗传基因座处整合到植物基因组中的具有两个T-DNA分子的转基因植物的选择。 组合物是包含含有感兴趣的基因，阳性选择标记基因和条件致死基因的T-DNA分子的新配置的DNA构建体。 本文公开的方法包括将植物细胞转化为包含本发明的DNA构建体，将植物细胞再生成植物并鉴定独立的转基因基因座，其中可选择标记基因或转基因元件可以在后代中分离。
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公开(公告)号：US09175298B2
公开(公告)日：2015-11-03
申请号：US11857000
申请日：2007-09-18
申请人： Toni Armstrong , Kenneth Barton , Laura J. Crow , Larry A. Gilbertson , Shihshieh Huang , Yong Huang , Brian J. Martinell , Michael W. Petersen , Kenneth J. Piller , Qi Wang , Xudong Ye
发明人： Toni Armstrong , Kenneth Barton , Laura J. Crow , Larry A. Gilbertson , Shihshieh Huang , Yong Huang , Brian J. Martinell , Michael W. Petersen , Kenneth J. Piller , Qi Wang , Xudong Ye
IPC分类号： C12N15/82
CPC分类号： C12N15/8205 , C12N15/8209 , C12N15/8263 , C12N15/8265 , C12N15/8289
摘要： The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independent transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.
摘要翻译： 提供的组合物和方法增强了在不同物理和遗传基因座处整合到植物基因组中的具有两个T-DNA分子的转基因植物的选择。 组合物是包含含有感兴趣的基因，阳性选择标记基因和条件致死基因的T-DNA分子的新配置的DNA构建体。 本文公开的方法包括将植物细胞转化为包含本发明的DNA构建体，将植物细胞再生成植物并鉴定独立的转基因基因座，其中可选择标记基因或转基因元件可以在后代中分离。
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公开(公告)号：US07960610B2
公开(公告)日：2011-06-14
申请号：US11856958
申请日：2007-09-18
申请人： Toni Armstrong , Kenneth Barton , Laura J. Crow , Larry A. Gilbertson , Shihshieh Huang , Yong Huang , Brian J. Martinell , Michael W. Petersen , Kenneth J. Piller , Qi Wang , Xudong Ye
发明人： Toni Armstrong , Kenneth Barton , Laura J. Crow , Larry A. Gilbertson , Shihshieh Huang , Yong Huang , Brian J. Martinell , Michael W. Petersen , Kenneth J. Piller , Qi Wang , Xudong Ye
IPC分类号： C12N15/82
CPC分类号： C12N15/8205 , C12N15/8209 , C12N15/8263 , C12N15/8265 , C12N15/8289
摘要： The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independent transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.
摘要翻译： 提供的组合物和方法增强了在不同物理和遗传基因座处整合到植物基因组中的具有两个T-DNA分子的转基因植物的选择。 组合物是包含含有感兴趣的基因，阳性选择标记基因和条件致死基因的T-DNA分子的新配置的DNA构建体。 本文公开的方法包括将植物细胞转化为包含本发明的DNA构建体，将植物细胞再生成植物并鉴定独立的转基因基因座，其中可选择标记基因或转基因元件可以在后代中分离。
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4.发明授权Methods for enhancing segregation of transgenes in plants and compositions thereof 有权用于增强植物中转基因分离的方法及其组合物公开(公告)号：US07288694B2
公开(公告)日：2007-10-30
申请号：US10190217
申请日：2002-07-05
申请人： Toni Armstrong , Kenneth Barton , Laura J. Crow , Larry A. Gilbertson , Shihshieh Huang , Yong Huang , Brian J. Martinell , Michael W. Petersen , Kenneth J. Piller , Qi Wang , Xudong Ye
发明人： Toni Armstrong , Kenneth Barton , Laura J. Crow , Larry A. Gilbertson , Shihshieh Huang , Yong Huang , Brian J. Martinell , Michael W. Petersen , Kenneth J. Piller , Qi Wang , Xudong Ye
IPC分类号： C12N15/82
CPC分类号： C12N15/8205 , C12N15/8209 , C12N15/8263 , C12N15/8265 , C12N15/8289
摘要： The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independant transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.
摘要翻译： 提供的组合物和方法增强了在不同物理和遗传基因座处整合到植物基因组中的具有两个T-DNA分子的转基因植物的选择。 组合物是包含含有感兴趣的基因，阳性选择标记基因和条件致死基因的T-DNA分子的新配置的DNA构建体。 本文公开的方法包括将植物细胞转化为包含本发明的DNA构建体，将植物细胞再生成植物并鉴定独立的转基因基因座，其中可选择标记基因或转基因元件可以在后代中分离。
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公开(公告)号：US20080064866A1
公开(公告)日：2008-03-13
申请号：US11856978
申请日：2007-09-18
申请人： Toni Armstrong , Kenneth Barton , Laura Crow , Larry Gilbertson , Shihshieh Huang , Yong Huang , Brian Martinell , Michael Petersen , Kenneth Piller , Qi Wang , Xudong Ye
发明人： Toni Armstrong , Kenneth Barton , Laura Crow , Larry Gilbertson , Shihshieh Huang , Yong Huang , Brian Martinell , Michael Petersen , Kenneth Piller , Qi Wang , Xudong Ye
IPC分类号： C07H21/04
CPC分类号： C12N15/8205 , C12N15/8209 , C12N15/8263 , C12N15/8265 , C12N15/8289
摘要： The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independant transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.
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公开(公告)号：US20080066202A1
公开(公告)日：2008-03-13
申请号：US11856958
申请日：2007-09-18
申请人： Toni Armstrong , Kenneth Barton , Laura Crow , Larry Gilbertson , Shihshieh Huang , Yong Huang , Brian Martinell , Michael Petersen , Kenneth Piller , Qi Wang , Xudong Ye
发明人： Toni Armstrong , Kenneth Barton , Laura Crow , Larry Gilbertson , Shihshieh Huang , Yong Huang , Brian Martinell , Michael Petersen , Kenneth Piller , Qi Wang , Xudong Ye
CPC分类号： C12N15/8205 , C12N15/8209 , C12N15/8263 , C12N15/8265 , C12N15/8289
摘要： The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independant transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.
摘要翻译： 提供的组合物和方法增强了在不同物理和遗传基因座处整合到植物基因组中的具有两个T-DNA分子的转基因植物的选择。 组合物是包含含有感兴趣的基因，阳性选择标记基因和条件致死基因的T-DNA分子的新配置的DNA构建体。 本文公开的方法包括将植物细胞转化为包含本发明的DNA构建体，将植物细胞再生成植物并鉴定独立的转基因基因座，其中可选择标记基因或转基因元件可以在后代中分离。
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公开(公告)号：US20080070296A1
公开(公告)日：2008-03-20
申请号：US11857000
申请日：2007-09-18
申请人： Toni Armstrong , Kenneth Barton , Laura Crow , Larry Gilbertson , Shihshieh Huang , Yong Huang , Brian Martinell , Michael Petersen , Kenneth Piller , Qi Wang , Xudong Ye
发明人： Toni Armstrong , Kenneth Barton , Laura Crow , Larry Gilbertson , Shihshieh Huang , Yong Huang , Brian Martinell , Michael Petersen , Kenneth Piller , Qi Wang , Xudong Ye
IPC分类号： C12N15/00
CPC分类号： C12N15/8205 , C12N15/8209 , C12N15/8263 , C12N15/8265 , C12N15/8289
摘要： The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independant transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.
摘要翻译： 提供的组合物和方法增强了在不同物理和遗传基因座处整合到植物基因组中的具有两个T-DNA分子的转基因植物的选择。 组合物是包含含有感兴趣的基因，阳性选择标记基因和条件致死基因的T-DNA分子的新配置的DNA构建体。 本文公开的方法包括将植物细胞转化为包含本发明的DNA构建体，将植物细胞再生成植物并鉴定独立的转基因基因座，其中可选择标记基因或转基因元件可以在后代中分离。
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公开(公告)号：US20090170411A1
公开(公告)日：2009-07-02
申请号：US12005925
申请日：2007-12-29
申请人： Kenneth Barton
发明人： Kenneth Barton
摘要： A flexible micropolishing machine for micropolishing a piston ring comprising an arbor for accepting at least one piston ring, a means for rotating the arbor, a flexible roller arm located adjacent the arbor, and an indexable abrasive material located between the flexible roller arm and the at least one piston ring wherein the flexible roller arm is movable toward the arbor such that flexible roller arm applies a micropolishing pressure to the indexable abrasive tape thereby micropolishing the piston ring.
摘要翻译： 一种用于对包括用于容纳至少一个活塞环的心轴的活塞环进行微孔抛光的柔性微抛光机，用于旋转心轴的装置，位于心轴附近的柔性辊臂以及位于柔性辊臂与at之间的可转位磨料 至少一个活塞环，其中柔性辊臂可朝向心轴移动，使得柔性辊臂向可转位磨料带施加微量的抛光压力，从而对活塞环进行微孔抛光。
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公开(公告)号：US5049003A
公开(公告)日：1991-09-17
申请号：US633472
申请日：1990-12-24
申请人： Kenneth Barton
发明人： Kenneth Barton
IPC分类号： F16L55/163 , F16L55/26
CPC分类号： F16L55/26 , F16L55/163
摘要： An apparatus and method are disclosed for repairing ruptures in underground conduits. An inflatable bladder and a flexible sleeve that is wrapped about at least a portion of the bladder are introduced into a conduit proximate a rupture therein. The bladder is inflated to urge the sleeve into engagement with the conduit such that the sleeve covers the rupture. The sleeve includes an adhesive bonding substance that adheres the sleeve to the conduit and is hardenable to bond the sleeve to the conduit.
摘要翻译： 公开了一种修复地下管道破裂的装置和方法。 围绕囊的至少一部分缠绕的可膨胀囊和柔性套管被引入靠近其中破裂的导管。 囊膨胀以促使套管与导管接合，使得套筒覆盖破裂。 套筒包括粘合剂粘合物质，其将套筒粘附到导管并且可硬化以将套管粘合到导管。
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10.发明申请公开(公告)号：US20060009548A1
公开(公告)日：2006-01-12
申请号：US11224824
申请日：2005-09-12
IPC分类号： C08K3/20
CPC分类号： D06M15/5075 , C08G63/6886 , C08L25/06 , C08L25/08 , C08L25/14 , C08L67/02 , D06M15/233 , D06P1/5221 , D06P1/5271 , D06P3/52 , C08L2666/06
摘要： An aqueous dispersion has an addition polymer (B) formed in situ on a sulfopolyester (A). The sulfopolyester (A) is formed as a polycondensation product of first and second reaction mixtures. The first reaction mixture includes at least about 70 mol. % of terephthalic acid or an ester thereof, and about 4-20 mol. % of a difunctional ester forming sulfomonomer containing at least one metal sulfonate group attached to an aromatic nucleus, wherein the functional groups are selected from the group consisting of hydroxyls, carboxyls and alkyl esters. The second reaction mixture includes at least two different glycols, at least about 20 mol. % of the second reaction mixture being ethylene glycol. The addition polymer (B) is formed in situ on the sulfopolyester (B) by polymerizing styrene monomer.
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