公开(公告)号：US20090209037A1

公开(公告)日：2009-08-20

申请号：US12261999

申请日：2008-10-30

申请人： Toshiaki Tagawa ,  David Andrew Miller ,  Eric Perouzel ,  Karl Murray ,  Michelle Manvell ,  Eric Alton ,  David Matthews ,  Willie Russell

发明人： Toshiaki Tagawa ,  David Andrew Miller ,  Eric Perouzel ,  Karl Murray ,  Michelle Manvell ,  Eric Alton ,  David Matthews ,  Willie Russell

IPC分类号： C12N15/88 ,  C12N15/00

CPC分类号： C07K14/005 ,  A61K9/1272 ,  A61K47/645 ,  A61K47/6901 ,  A61K48/00 ,  C12N15/88 ,  C12N2710/10322

摘要： A nucleic acid delivery complex is provided which comprises a condensed polypeptide/nucleic acid complex and a cationic lipid wherein the complex comprises (a) a nucleic acid sequence of interest (NOI); and (b) one or more viral nucleic acid packaging polypeptides, or derivatives thereof, said polypeptides or derivatives thereof being (i) capable of binding to the NOI; and (ii) capable of condensing the NOI; and wherein the NOI is heterologous to the polypeptide. Also provided is a method of introducing an NOI into a cell using the delivery vector.

摘要翻译： 提供了包含缩合多肽/核酸复合物和阳离子脂质的核酸递送复合物，其中所述复合物包含（a）感兴趣的核酸序列（NOI）; 和（b）一种或多种病毒核酸包装多肽或其衍生物，所述多肽或其衍生物是（i）能够结合NOI; 和（ii）能够冷凝NOI; 并且其中所述NOI与所述多肽是异源的。 还提供了使用递送载体将NOI引入细胞的方法。