公开(公告)号：US08557523B2

公开(公告)日：2013-10-15

申请号：US12738413

申请日：2008-10-17

申请人： Toshihiro Yonekawa ,  Tsugunori Notomi ,  Hidetoshi Kanda ,  Norimitsu Hosaka

发明人： Toshihiro Yonekawa ,  Tsugunori Notomi ,  Hidetoshi Kanda ,  Norimitsu Hosaka

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6876 ,  C12Q1/6851 ,  C12Q2561/113 ,  C12Q2527/143 ,  C12Q2527/113 ,  C12Q2545/101 ,  C12Q2537/149 ,  C12Q2531/119

摘要： The object of the present invention is to provide a method that allows stable amplification of an internal standard material while maintaining an accurate assay value for a target nucleic acid in a nucleic acid detection system involving the use of an internal standard material and a reagent kit used therefor. The present invention relates to a method for nucleic acid amplification comprising preventing an internal standard amplification product from affecting amplification reaction of a target nucleic acid by performing amplification of an internal standard material prior to amplification of the target nucleic acid in the method for amplifying a target nucleic acid in a sample using an internal standard material and a reagent and reagent kit used therefor.

摘要翻译： 本发明的目的是提供一种允许内标物质稳定扩增的方法，同时在涉及使用内标物质和使用的试剂盒的核酸检测系统中保持目标核酸的准确测定值 因此。 本发明涉及一种用于核酸扩增的方法，包括通过在靶核酸扩增方法中扩增靶核酸之前，通过扩增内标物质来防止内标扩增产物影响靶核酸的扩增反应 使用内标物质的样品中的核酸和用于其的试剂和试剂盒。

公开(公告)号：US20100233715A1

公开(公告)日：2010-09-16

申请号：US12738413

申请日：2008-10-17

申请人： Toshihiro Yonekawa ,  Tsugunori Notomi ,  Hidetoshi Kanda ,  Norimitsu Hosaka

发明人： Toshihiro Yonekawa ,  Tsugunori Notomi ,  Hidetoshi Kanda ,  Norimitsu Hosaka

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6876 ,  C12Q1/6851 ,  C12Q2561/113 ,  C12Q2527/143 ,  C12Q2527/113 ,  C12Q2545/101 ,  C12Q2537/149 ,  C12Q2531/119

摘要： The object of the present invention is to provide a method that allows stable amplification of an internal standard material while maintaining an accurate assay value for a target nucleic acid in a nucleic acid detection system involving the use of an internal standard material and a reagent kit used therefor. The present invention relates to a method for nucleic acid amplification comprising preventing an internal standard amplification product from affecting amplification reaction of a target nucleic acid by performing amplification of an internal standard material prior to amplification of the target nucleic acid in the method for amplifying a target nucleic acid in a sample using an internal standard material and a reagent and reagent kit used therefor.

摘要翻译： 本发明的目的是提供一种允许内标物质稳定扩增的方法，同时在涉及使用内标物质和使用的试剂盒的核酸检测系统中保持目标核酸的准确测定值 因此。 本发明涉及一种用于核酸扩增的方法，包括通过在靶核酸扩增方法中扩增靶核酸之前，通过扩增内标物质来防止内标扩增产物影响靶核酸的扩增反应 使用内标物质的样品中的核酸和用于其的试剂和试剂盒。