公开(公告)号：US5362635A

公开(公告)日：1994-11-08

申请号：US82459

申请日：1993-06-25

申请人： Toshiki Hirose ,  Minoru Tsuruta ,  Koji Tamura ,  Yoshitomo Uehara ,  Harufumi Miwa

发明人： Toshiki Hirose ,  Minoru Tsuruta ,  Koji Tamura ,  Yoshitomo Uehara ,  Harufumi Miwa

IPC分类号： C12P13/14 ,  B01D19/00 ,  C12M1/21 ,  C12R1/13 ,  C12P13/04 ,  C12P1/04 ,  C12P7/14

CPC分类号： C12M29/18 ,  B01D19/0068 ,  C12M29/20 ,  C12M47/02 ,  Y10S435/812 ,  Y10S435/813 ,  Y10S435/819

摘要： Amino acid fermentation is conducted by fermenting bacterial cells in a culture medium in a fermentor and separating fermentation solution withdrawn from the fermentor into a solution containing said bacterial cells and a solution not containing bacterial cells by a cell separator. The solution containing said bacterial cells being circulated from said cell separator to said fermenter by circulating means to perform amino acid fermentation continuously, and bubbles being removed from said fermentation solution by a bubble separator before said fermentation solution is fed to said circulating means and said cell separator.

摘要翻译： 氨基酸发酵通过在发酵罐中的培养基中发酵细菌细胞并将从发酵罐中取出的发酵溶液分离成含有细菌细胞的溶液和细胞分离器不含细菌细胞的溶液进行。 含有所述细菌细胞的溶液通过循环装置从所述细胞分离器循环到所述发酵罐，以连续进行氨基酸发酵，并且在将所述发酵溶液进料到所述循环装置和所述细胞之前通过气泡分离器从所述发酵溶液中除去气泡 分隔器。

公开(公告)号：US5260216A

公开(公告)日：1993-11-09

申请号：US764210

申请日：1991-09-23

申请人： Toshiki Hirose ,  Minoru Tsuruta ,  Koji Tamura ,  Yoshitomo Uehara ,  Harufumi Miwa

发明人： Toshiki Hirose ,  Minoru Tsuruta ,  Koji Tamura ,  Yoshitomo Uehara ,  Harufumi Miwa

IPC分类号： C12P13/14 ,  B01D19/00 ,  C12M1/21 ,  C12R1/13 ,  C12M1/12

CPC分类号： C12M29/18 ,  B01D19/0068 ,  C12M29/20 ,  C12M47/02 ,  Y10S435/812 ,  Y10S435/813 ,  Y10S435/819

摘要： Amino acid fermentation is conducted by fermenting using bacterial cells in a culture medium in a fermentor and separating fermentation solution withdrawn from the fermentor into a solution containing the bacterial cells and a solution not containing bacterial cells by a cell separator, the solution containing the bacterial cells being circulated from the cell separator to the fermenter by a circulating device to perform amino acid fermentation continuously, and bubbles being removed from the fermentation solution by a bubble separator before the fermentation solution is fed to the circulating device and the cell separator.

摘要翻译： 氨基酸发酵通过使用发酵罐中的培养基中的细菌细胞进行发酵，并将从发酵罐中取出的发酵液分离为含有细菌细胞的溶液和不含细菌细胞的溶液，细胞细胞的溶液 通过循环装置从循环装置循环到发酵罐中，连续进行氨基酸发酵，并且在将发酵溶液送入循环装置和细胞分离器之前，通过气泡分离器从发酵液中除去气泡。

公开(公告)号：US5476573A

公开(公告)日：1995-12-19

申请号：US980291

申请日：1992-11-23

申请人： Toshiki Hirose ,  Koji Shimazaki ,  Takashi Nakamura ,  Atsuo Shiraishi ,  Hiroki Kawashima ,  Tatsuya Nakayama

发明人： Toshiki Hirose ,  Koji Shimazaki ,  Takashi Nakamura ,  Atsuo Shiraishi ,  Hiroki Kawashima ,  Tatsuya Nakayama

IPC分类号： C12M1/00 ,  C12M1/04 ,  C12M1/21 ,  C12N1/00

CPC分类号： C12M41/02 ,  C12M29/02 ,  C12M29/20 ,  Y10S435/812

摘要： An apparatus and method for defoaming aerobic fermentation cultures is provided, where the amount of liquid culture medium used is greater than that in conventional aerobic cultures (70% or more of the total capacity of the culture tank) without lowering the productivity of the intended, desired product. In the present apparatus and method, a device for separating vapor from liquid of a foam is in fluid communication with the fermentation tank, a device for condensing residual liquid of the vapor received from the separating device is in fluid communication with the separating device, and a sensor for detecting foams is provided in a location along the fluid communication pathway. In the defoaming apparatus, an optional defoaming device is provided, which may be based on either a rotary body rotating at a high speed by use of an electric motor which beats the foams, or on a centrifugal atomizer. The defoaming device may be provided between the vapor-liquid separating device and the condensing device.

摘要翻译： 提供了一种用于消泡需氧发酵培养物的装置和方法，其中使用的液体培养基的量大于常规需氧培养物（培养罐的总容量的70％以上）的量，而不降低预期的， 所需产品。 在本发明的装置和方法中，用于将泡沫中的蒸汽与液体分离的装置与发酵罐流体连通，用于冷凝从分离装置接收的蒸汽的残留液体与分离装置流体连通的装置， 用于检测泡沫的传感器设置在沿着流体连通路径的位置。 在消泡装置中，提供了可选的消泡装置，其可以基于通过使用打开泡沫的电动机高速旋转的旋转体或离心式雾化器。 可以在气液分离装置和冷凝装置之间设置消泡装置。