摘要:
An alkaline protease having an amino acid with one or more amino acid residues selected from among those located at (a) position 63, (b) position 89, (c) position 120, (d) positions 63 and 187, (e) position 226, (f) position 296, (g) position 304 of the amino acid sequence of SEQ ID NO: 1, or at positions corresponding to these positions, are the following amino acid residues, respectively: (a): serine, (b): histidine, (c): arginine, (d): serine,(e): tyrosine, (f): valine, and (g): serine; and a gene encoding the alkaline protease.
摘要翻译:一种具有一个或多个氨基酸残基的氨基酸的碱性蛋白酶,其选自(a)位置63,(b)位置89,(c)位置120,(d)位置63和187,(e)位置 226,(f)位置296,(g)SEQ ID NO:1的氨基酸序列的位置304或与这些位置相应的位置分别是以下氨基酸残基:(a):丝氨酸,(b ):组氨酸,(c):精氨酸,(d):丝氨酸,(e):酪氨酸,(f):缬氨酸和(g):丝氨酸; 和编码碱性蛋白酶的基因。
摘要:
An alkaline protease having an amino acid, wherein one or more amino acid residues selected from among those located at (a) position 63, (b) position 89, (c) position 120, (d) positions 63 and 187, (e) position 226, (f) position 296, (g) position 304 of the amino acid sequence of SEQ NO; 1, or at positions corresponding to these positions are the following amino acid residues, respectively: (a): serine, (b): histidine, (c): arginine, (d): serine, (e): tyrosine, (f): valine, and (g): serine; and a gene encoding the alkaline protease. The alkaline protease has for example, excellent detergency against complex soil and has improved thermal stability.
摘要翻译:具有氨基酸的碱性蛋白酶,其中选自位于(a)位置63,(b)位置89,(c)位置120,(d)位置63和187的那些中的一个或多个氨基酸残基,(e) 位置226,(f)位置296,(g)SEQ ID NO:1的氨基酸序列的位置304; (a):丝氨酸,(b):组氨酸,(c):精氨酸,(d):丝氨酸,(e):酪氨酸,(f ):缬氨酸和(g):丝氨酸; 和编码碱性蛋白酶的基因。 碱性蛋白酶具有例如对复杂土壤的优异的去污力并且具有改善的热稳定性。
摘要:
Provided in the present invention is an alkaline protease wherein an amino acid residue at (a) position 65, (b) position 101, (c) position 163, (d) position 170, (e) position 171, (f) position 273, (g) position 320, (h) position 359 or (i) position 387 of SEQ. ID NO:1 or at a position corresponding thereto has been selected from the following amino acid residues: position (a): proline, position (b): asparagine, position (c): histidine, aspartic acid, phenylalanine, lysine, asparagine, serine, isoleucine, leucine, glutamine, threonine and valine, position (d): valine and leucine, position (e): alanine, glutamic acid, glycine and threonine, position (f): isoleucine, glycine and threonine, position (g): phenylalanine, valine, threonine, leucine, isoleucine and glycine, position (h): serine, leucine, valine, isoleucine and glutamine, position (i): alanine, lysine, glutamine, glutamic acid, arginine and histidine.The present invention makes it possible to efficiently produce and provide alkaline proteases having activity even in the presence of a highly concentrated fatty acid, and exhibiting excellent detergency for the removal of a complex stain containing protein, sebum and the like, and therefore being useful as an enzyme to be incorporated in a detergent.
摘要翻译:在本发明中提供的是碱性蛋白酶,其中(a)位置65,(b)位置101,(c)位置163,(d)位置170,(e)位置171,(f) ,(g)位置320,(h)位置359或(i)SEQ ID NO: 位置(a):脯氨酸,位置(b):天冬酰胺,位置(c):组氨酸,天冬氨酸,苯丙氨酸,赖氨酸,天冬酰胺, 位置(d):缬氨酸和亮氨酸,位置(e):丙氨酸,谷氨酸,甘氨酸和苏氨酸,位置(f):异亮氨酸,甘氨酸和苏氨酸,位置(g) 位置(h):丝氨酸,亮氨酸,缬氨酸,异亮氨酸和谷氨酰胺,位置(i):丙氨酸,赖氨酸,谷氨酰胺,谷氨酸,精氨酸和组氨酸,位置(h):缬氨酸,缬氨酸,苏氨酸,亮氨酸,异亮氨酸和甘氨酸。 本发明使得可以有效地生产和提供具有活性的碱性蛋白酶,即使在高度浓缩的脂肪酸的存在下也具有优异的去污力来去除含有蛋白质,皮脂等的复合污渍,因此可用作 要掺入洗涤剂中的酶。
摘要:
An alkaline protease wherein an amino acid residue at (a) position 65, (b) position 101, (c) position 163, (d) position 17-0, (e) position 171, (f) position 273, (g) position 320, (h) position 359 or (i) position 387 of SEQ. ID NO: 2 or at a position corresponding thereto has been selected from the following amino acid residues: position (a): proline, position (b): asparagine, position (e): histidine, aspartic acid, phenylalanine, lysine, asparagine, seine, isoleucine, leucine, glutamine, threonine and valine, position (d): valine and leucine, position (e): alanine, glutamic acid, glycine and threonine, position (K): isoleucine, glycine and threonine, position (g): phenylalanine, valine, threonine, leucine, isoleucine and glycine, position (h): seine, leucine, valine, isoleucine and glutamine, position (i): alanine, lysine, glutamine, glutamic acid, arginine and histidine. A method to an alkaline protease having activity even in the presence of a highly concentrated fatty acid, and exhibiting excellent detergency for the removal of a complex stain containing protein, sebum and the like, and therefore useful as an ingredient in a detergent.
摘要翻译:(a)位置65,(b)位置101,(c)位置163,(d)位置17-0,(e)位置171,(f)位置273,(g) 位置320,(h)位置359或(i)SEQ ID NO: 位置(a):脯氨酸,位置(b):天冬酰胺,位置(e):组氨酸,天冬氨酸,苯丙氨酸,赖氨酸,天冬酰胺, (e):丙氨酸,谷氨酸,甘氨酸和苏氨酸,位置(K):异亮氨酸,甘氨酸和苏氨酸,位置(g):缬氨酸和亮氨酸, (i):丙氨酸,赖氨酸,谷氨酰胺,谷氨酸,精氨酸和组氨酸,位置(h):ine碱,亮氨酸,缬氨酸,异亮氨酸和谷氨酰胺。 一种碱性蛋白酶的方法,即使在高度浓缩的脂肪酸的存在下也具有活性,并且对于去除含有蛋白质,皮脂等的复合污渍具有优异的去污力,因此可用作洗涤剂中的成分。
摘要:
Provided in the present invention is an alkaline protease wherein an amino acid residue at (a) position 65, (b) position 101, (c) position 163, (d) position 170, (e) position 171, (f) position 273, (g) position 320, (h) position 359 or (i) position 387 of SEQ. ID NO:1 or at a position corresponding thereto has been selected from the following amino acid residues: position (a): proline, position (b): asparagine, position (c): histidine, aspartic acid, phenylalanine, lysine, asparagine, serine, isoleucine, leucine, glutamine, threonine and valine, position (d): valine and leucine, position (e): alanine, glutamic acid, glycine and threonine, position (f): isoleucine, glycine and threonine, position (g): phenylalanine, valine, threonine, leucine, isoleucine and glycine, position (h): serine, leucine, valine, isoleucine and glutamine, position (i): alanine, lysine, glutamine, glutamic acid, arginine and histidine.The present invention makes it possible to efficiently produce and provide alkaline proteases having activity even in the presence of a highly concentrated fatty acid, and exhibiting excellent detergency for the removal of a complex stain containing protein, sebum and the like, and therefore being useful as an enzyme to be incorporated in a detergent.
摘要翻译:在本发明中提供的是碱性蛋白酶,其中(a)位置65,(b)位置101,(c)位置163,(d)位置170,(e)位置171,(f) ,(g)位置320,(h)位置359或(i)SEQ ID NO: 位置(a):脯氨酸,位置(b):天冬酰胺,位置(c):组氨酸,天冬氨酸,苯丙氨酸,赖氨酸,天冬酰胺, 位置(d):缬氨酸和亮氨酸,位置(e):丙氨酸,谷氨酸,甘氨酸和苏氨酸,位置(f):异亮氨酸,甘氨酸和苏氨酸,位置(g) 位置(h):丝氨酸,亮氨酸,缬氨酸,异亮氨酸和谷氨酰胺,位置(i):丙氨酸,赖氨酸,谷氨酰胺,谷氨酸,精氨酸和组氨酸,位置(h):缬氨酸,缬氨酸,苏氨酸,亮氨酸,异亮氨酸和甘氨酸。 本发明使得可以有效地生产和提供具有活性的碱性蛋白酶,即使在高度浓缩的脂肪酸的存在下也具有优异的去污力来去除含有蛋白质,皮脂等的复合污渍,因此可用作 要掺入洗涤剂中的酶。
摘要:
The present invention relates to genes expressing an alkaline protease having a prepro sequence, vectors containing the same, and transformants containing said vector. The present invention also relates to a method for producing an alkaline protease.
摘要:
An alkaline protease having an amino acid with one or more amino acid residues selected from among those located at (a) position 15, (b) position 16, (c) position 166, (d) position 167, (e) position 187, (f) position 346, and (g) position 405 of the amino acid sequence of SEQ No; 1, or at positions corresponding to these positions, are the following amino acid residues, respectively: (a): histidine, (b): threonine or glutamine, (c): glycine, (d): valine, (e): serine, (f): arginine, and (g); and a gene encoding the alkaline protease.
摘要翻译:一种具有一个或多个氨基酸残基的氨基酸的碱性蛋白酶,其选自位于(a)位置15,(b)位置16,(c)位置166,(d)位置167,(e)位置187, (f)位置346和(g)SEQ ID NO:1的氨基酸序列的位置405; 分别为:(a):组氨酸,(b):苏氨酸或谷氨酰胺,(c):甘氨酸,(d):缬氨酸,(e)):丝氨酸 ,(f):精氨酸和(g); 和编码碱性蛋白酶的基因。
摘要:
An alkaline protease having an amino acid sequence, wherein one or more amino acid residues selected from those located at (a) position 15, (b) position 16, (c) position 166, (d) position 167, (e) position 187, (f) position 346, and (g) position 405 of the amino acid sequence of SEQ ID NO: 1, or at positions corresponding to these positions are the following amino acid residues, respectively: (a): histidine, (b): threonine or glutamine, (c): glycine, (d) valine, (e): serine, (f): arginine, and (g): aspartic acid; and a gene encoding the alkaline protease. The alkaline protease exhibits for example excellent detergency against complex soil, and improved protein secretion amount and specific activity, and attains enhanced productivity.
摘要翻译:一种具有氨基酸序列的碱性蛋白酶,其中选自位于(a)位置15,(b)位置16,(c)位置166,(d)位置167,(e)位置187的氨基酸残基中的一个或多个氨基酸残基 ,(f)位置346和(g)SEQ ID NO:1的氨基酸序列的位置405或对应于这些位置的位置分别是以下氨基酸残基:(a):组氨酸,(b) :苏氨酸或谷氨酰胺,(c):甘氨酸,(d)缬氨酸,(e):丝氨酸,(f):精氨酸,和(g):天冬氨酸; 和编码碱性蛋白酶的基因。 碱性蛋白酶表现出对复杂土壤的优异的去污力,以及提高的蛋白质分泌量和比活性,并且提高了生产率。
摘要:
The present invention relates to genes expressing an alkaline protease having a prepro sequence, vectors containing the same, and transformants containing said vector. The present invention also relates to a method for producing an alkaline protease.
摘要:
The present invention provides an alkaline protease exhibiting high detergency and productivity, which is obtained by further enhancing the detergency of an alkaline protease effective against complex dirt and enhancing the specific activity.