公开(公告)号：US20200017907A1

公开(公告)日：2020-01-16

申请号：US16417023

申请日：2019-05-20

Applicant: Twist Bioscience Corporation

Inventor： Ramsey Ibrahim ZEITOUN ,  Siyuan CHEN ,  Richard GANTT ,  Kristin D. BUTCHER ,  E. Hutson CHILTON

IPC: C12Q1/6869 ,  G16B40/10 ,  C40B30/10 ,  C40B70/00

Abstract: Provided herein are compositions, methods and systems relating to libraries of polynucleotides such that the libraries allow for accurate and efficient hybridization after binding to target sequences. Further provided herein are probes, blockers, additives, buffers, and methods that result in improved hybridization. Such compositions and methods are useful for improvement of Next Generation Sequencing applications, such as reducing off-target binding or reducing workflow times.

公开(公告)号：US20210002710A1

公开(公告)日：2021-01-07

申请号：US16798275

申请日：2020-02-21

Applicant: Twist Bioscience Corporation

Inventor： Richard GANTT ,  Siyuan CHEN

IPC: C12Q1/6813 ,  C40B70/00 ,  C12Q1/6876 ,  C12N15/10

Abstract: Provided herein are compositions and methods for next generation sequencing using universal polynucleotide adapters. Further provided are universal adapters using locked nucleic acids or bridged nucleic acids. Further provided are barcoded primers of reduced length for extension of universal adapters. Further provided herein are universal adapter blockers.

公开(公告)号：US20240043920A1

公开(公告)日：2024-02-08

申请号：US18341544

申请日：2023-06-26

Applicant: Twist Bioscience Corporation

Inventor： Ramsey Ibrahim ZEITOUN ,  Siyuan CHEN ,  Richard GANTT ,  Kristin D. BUTCHER ,  E. Hutson CHILTON

IPC: C12Q1/6869 ,  G16B40/10 ,  C40B30/10 ,  C40B70/00

CPC classification number: C12Q1/6869 ,  G16B40/10 ,  C40B30/10 ,  C40B70/00

Abstract: Provided herein are compositions, methods and systems relating to libraries of polynucleotides such that the libraries allow for accurate and efficient hybridization after binding to target sequences. Further provided herein are probes, blockers, additives, buffers, and methods that result in improved hybridization. Such compositions and methods are useful for improvement of Next Generation Sequencing applications, such as reducing off-target binding or reducing workflow times.

公开(公告)号：US20210207197A1

公开(公告)日：2021-07-08

申请号：US17154906

申请日：2021-01-21

Applicant: Twist Bioscience Corporation

Inventor： Richard GANTT ,  Siyuan CHEN

IPC: C12Q1/6813 ,  C12N15/10 ,  C12Q1/6876 ,  C40B70/00

Abstract: Provided herein are compositions and methods for next generation sequencing using universal polynucleotide adapters. Further provided are universal adapters using locked nucleic acids or bridged nucleic acids. Further provided are barcoded primers of reduced length for extension of universal adapters. Further provided herein are universal adapter blockers.

公开(公告)号：US20230323449A1

公开(公告)日：2023-10-12

申请号：US18066953

申请日：2022-12-15

Applicant: Twist Bioscience Corporation

Inventor： Richard GANTT ,  Adriana ARNESON

IPC: C12Q1/6874 ,  C12Q1/6855

CPC classification number: C12Q1/6874 ,  C12Q1/6855 ,  C12Q2600/16 ,  C12Q2600/156

Abstract: Testing the many hypotheses from genomics and systems biology experiments demands accurate and cost-effective gene and genome synthesis. Here we describe a microchip-based technology for multiplex gene synthesis. Pools of thousands of ‘construction’ oligonucleotides and tagged complementary ‘selection’ oligonucleotides are synthesized on photo-programmable microfluidic chips, released, amplified and selected by hybridization to reduce synthesis errors ninefold. A one-step polymerase assembly multiplexing reaction assembles these into multiple genes. This technology enabled us to synthesize all 21 genes that encode the proteins of the Escherichia coli 30S ribosomal subunit, and to optimize their translation efficiency in vitro through alteration of codon bias. This is a significant step towards the synthesis of ribosomes in vitro and should have utility for synthetic biology in general.

公开(公告)号：US20220135965A1

公开(公告)日：2022-05-05

申请号：US17511324

申请日：2021-10-26

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： Richard GANTT ,  Adriana ARNESON

IPC: C12N15/10

Abstract: Provided herein are compositions and methods for Next Generation Sequencing. Further provided herein are compositions and methods for uniquely labeling molecules. Further provided herein are compositions and methods for synthesizing unique molecular identifiers.