公开(公告)号：US09476081B2

公开(公告)日：2016-10-25

申请号：US14451975

申请日：2014-08-05

Applicant: UCB PHARMA S.A.

Inventor： Katharine Lacy Cain ,  Shirley Jane Peters ,  Paul Edward Stephens

IPC: C12N15/63 ,  C12P21/00 ,  C07K14/47 ,  C07K16/00 ,  C07K16/18 ,  C07K16/40 ,  C12N9/02 ,  C12P21/02

CPC classification number: C12P21/00 ,  C07K14/47 ,  C07K16/00 ,  C07K16/18 ,  C07K16/40 ,  C12N9/0004 ,  C12P21/02 ,  Y02P20/52

Abstract: The present invention relates to a recombinant host cell, wherein the cell is modified to increase the expression levels of Ero1 and XBP1 relative to the expression levels of Ero1 and XBP1 in an unmodified cell. The present invention also relates to a method of producing a recombinant protein of interest comprising expressing the recombinant protein of interest in the recombinant host cell.

公开(公告)号：US20150025128A1

公开(公告)日：2015-01-22

申请号：US14451975

申请日：2014-08-05

Applicant: UCB PHARMA S.A.

Inventor： Katharine Lacy Cain ,  Shirley Jane Peters ,  Paul Edward Stephens

IPC: C12P21/00

CPC classification number: C12P21/00 ,  C07K14/47 ,  C07K16/00 ,  C07K16/18 ,  C07K16/40 ,  C12N9/0004 ,  C12P21/02 ,  Y02P20/52

Abstract: The present invention relates to a recombinant host cell, wherein the cell is modified to increase the expression levels of Ero1 and XBP1 relative to the expression levels of Ero1 and XBP1 in an unmodified cell. The present invention also relates to a method of producing a recombinant protein of interest comprising expressing the recombinant protein of interest in the recombinant host cell.

Abstract translation: 本发明涉及重组宿主细胞，其中修饰细胞以相对于未修饰的细胞中Ero1和XBP1的表达水平增加Ero1和XBP1的表达水平。 本发明还涉及生产重组蛋白质的方法，包括在重组宿主细胞中表达重组蛋白质。