-
公开(公告)号：US20180100143A1
公开(公告)日：2018-04-12
申请号：US15836220
申请日：2017-12-08
申请人： uniQure IP B.V.
CPC分类号： C12N7/00 , B01D61/145 , B01D61/16 , B01D71/10 , B01D2311/04 , B01D2311/26 , B01D2311/2623 , B01D2311/2649 , C12N7/02 , C12N2710/14051 , C12N2750/14051 , C12N2750/14332 , C12N2750/14351
摘要： The present invention relates to a separation of viruses of an essentially spherical shape from viruses with a rod-like shape that are comprised in a sample, wherein the sample comprising the viruses is subjected to filtration.
-
公开(公告)号：US20170321290A1
公开(公告)日：2017-11-09
申请号：US15531095
申请日：2015-11-27
申请人： uniQure IP B.V.
CPC分类号： C12Q1/701 , C12N2710/14021 , C12N2710/14111 , C12N2750/14111 , C12N2750/14121 , C12Q1/6851 , C12Q1/6869
摘要： The current invention relates to nucleic acid impurities in a composition comprising a parvoviral vector. In particular, the current invention shows that DNA impurities are not randomly encapsulated within a parvoviral virion. The invention therefore relates to a method for identifying and quantifying a nucleic acid impurity in a composition comprising a parvoviral vector. Finally, the current invention relates to method of determining whether a composition comprising a parvoviral vector is regarded as clinically pure.
-
3.发明申请公开(公告)号：US20170152525A1
公开(公告)日：2017-06-01
申请号：US15424546
申请日：2017-02-03
申请人： uniQure IP B.V.
发明人： Wilhelmus Theodorus Johannes Maria Christiaan HERMENS , Saskia Jacoba Petronella HAAST , Dennis Johan BIESMANS , Andrew Christian BAKKER
IPC分类号： C12N15/86 , C12N7/00 , C07K14/005
CPC分类号： C12N15/86 , C07K14/005 , C12N7/00 , C12N2710/14043 , C12N2710/14044 , C12N2710/14143 , C12N2710/14144 , C12N2710/14152 , C12N2750/14121 , C12N2750/14122 , C12N2750/14143 , C12N2750/14151 , C12N2750/14152 , C12N2800/105 , C12N2800/50
摘要： The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AA V) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins.
-
4.发明申请公开(公告)号：US20150307898A2
公开(公告)日：2015-10-29
申请号：US14603469
申请日：2015-01-23
申请人： UNIQURE IP B.V.
发明人： Wilhelmus Theodorus Johannes Maria Christiaan HERMENS , Saskia Jacoba Petronella HAAST , Dennis Johan BIESMANS , Andrew Christian BAKKER
IPC分类号： C12N15/86 , C12N7/00 , C07K14/005
CPC分类号： C12N15/86 , C07K14/005 , C12N7/00 , C12N2710/14043 , C12N2710/14044 , C12N2710/14143 , C12N2710/14144 , C12N2710/14152 , C12N2750/14121 , C12N2750/14122 , C12N2750/14143 , C12N2750/14151 , C12N2750/14152 , C12N2800/105 , C12N2800/50
摘要： The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AAV) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins.
-
5.发明申请公开(公告)号：US20130296532A1
公开(公告)日：2013-11-07
申请号：US13945505
申请日：2013-07-18
申请人： uniQure IP B.V.
发明人： Wilhelmus Theodorus Johannes Maria Christiaan HERMENS , Saskia Jacoba Petronella HAAST , Dennis Johan BIESMANS , Andrew Christian BAKKER
IPC分类号： C12N15/86
CPC分类号： C12N15/86 , C07K14/005 , C12N7/00 , C12N2710/14043 , C12N2710/14044 , C12N2710/14143 , C12N2710/14144 , C12N2710/14152 , C12N2750/14121 , C12N2750/14122 , C12N2750/14143 , C12N2750/14151 , C12N2750/14152 , C12N2800/105 , C12N2800/50
摘要： The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AAV) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins.
-
公开(公告)号：US20190218523A1
公开(公告)日：2019-07-18
申请号：US16359593
申请日：2019-03-20
申请人： uniQure IP B.V.
CPC分类号： C12N7/00 , B01D61/145 , B01D61/16 , B01D71/10 , B01D2311/04 , B01D2311/26 , B01D2311/2623 , B01D2311/2649 , C12N7/02 , C12N2710/14051 , C12N2750/14051 , C12N2750/14332 , C12N2750/14351
摘要： The present invention relates to a separation of viruses of an essentially spherical shape from viruses with a rod-like shape that are comprised in a sample, wherein the sample comprising the viruses is subjected to filtration.
-
7.发明申请公开(公告)号：US20180258448A1
公开(公告)日：2018-09-13
申请号：US15978669
申请日：2018-05-14
申请人： uniQure IP B.V.
发明人： Wilhelmus Theodorus Johannes Maria Christiaan HERMENS , Saskia Jacoba Petronella HAAST , Dennis Johan BIESMANS , Andrew Christian BAKKER
IPC分类号： C12N15/86 , C07K14/005 , C12N7/00
CPC分类号： C12N15/86 , C07K14/005 , C12N7/00 , C12N2710/14043 , C12N2710/14044 , C12N2710/14143 , C12N2710/14144 , C12N2710/14152 , C12N2750/14121 , C12N2750/14122 , C12N2750/14143 , C12N2750/14151 , C12N2750/14152 , C12N2800/105 , C12N2800/50
摘要： The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AA V) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins.
-
8.发明申请公开(公告)号：US20170145440A1
公开(公告)日：2017-05-25
申请号：US15424560
申请日：2017-02-03
申请人： uniQure IP B.V.
发明人： Wilhelmus Theodorus Johannes Maria Christiaan HERMENS , Saskia Jacoba Petronella HAAST , Dennis Johan BIESMANS , Andrew Christian BAKKER
CPC分类号： C12N15/86 , C07K14/005 , C12N7/00 , C12N2710/14043 , C12N2710/14044 , C12N2710/14143 , C12N2710/14144 , C12N2710/14152 , C12N2750/14121 , C12N2750/14122 , C12N2750/14143 , C12N2750/14151 , C12N2750/14152 , C12N2800/105 , C12N2800/50
摘要： The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AA V) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins.
-
9.发明申请BACULOVIRAL VECTORS COMPRISING REPEATED CODING SEQUENCES WITH DIFFERENTIAL CODON BIASES 有权包含具有差异编码序列的差异化编码序列的生物样本载体公开(公告)号：US20140186926A1
公开(公告)日：2014-07-03
申请号：US14192101
申请日：2014-02-27
申请人： UniQure IP B.V.
CPC分类号： C12N7/00 , C07K14/005 , C12N15/86 , C12N2710/14044 , C12N2710/14143 , C12N2750/14121 , C12N2750/14122 , C12N2750/14143 , C12N2750/14152 , C12N2800/22 , C12N2800/50
摘要： The present invention relates to production of proteins in insect cells whereby repeated coding sequences are used in baculoviral vectors. In particular the invention relates to the production of parvoviral vectors that may be used in gene therapy and to improvements in expression of the viral rep proteins that increase the productivity of parvoviral vectors.
摘要翻译： 本发明涉及昆虫细胞中的蛋白质的制备，其中在杆状病毒载体中使用重复的编码序列。 特别地，本发明涉及可用于基因治疗的细小病毒载体的生产和提高细小病毒载体生产力的病毒rep蛋白的表达的改进。
-
公开(公告)号：US20220251520A1
公开(公告)日：2022-08-11
申请号：US17722275
申请日：2022-04-15
申请人： uniQure IP B.V.
IPC分类号： C12N7/00 , C07K14/005 , C12N15/86
摘要： The present invention relates to production of proteins in insect cells whereby repeated coding sequences are used in baculoviral vectors. In particular the invention relates to the production of parvoviral vectors that may be used in gene therapy and to improvements in expression of the viral rep proteins that increase the productivity of parvoviral vectors.
-
-
-
-
-
-
-
-
-
搜索结果
13 条记录 (耗时 0.017 秒)
