-
1.发明申请公开(公告)号：US20150140639A1
公开(公告)日：2015-05-21
申请号：US14603469
申请日：2015-01-23
申请人： UNIQURE IP B.V.
发明人： Wilhelmus Theodorus Johannes Maria Christiaan Maria Christ HERMENS , Saskia Jacoba Petronella HAAST , Dennis Johan BIESMANS , Andrew Christian BAKKER
IPC分类号： C12N15/86 , C12N7/00 , C07K14/005
CPC分类号： C12N15/86 , C07K14/005 , C12N7/00 , C12N2710/14043 , C12N2710/14044 , C12N2710/14143 , C12N2710/14144 , C12N2710/14152 , C12N2750/14121 , C12N2750/14122 , C12N2750/14143 , C12N2750/14151 , C12N2750/14152 , C12N2800/105 , C12N2800/50
摘要： The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AAV) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins.
-
2.发明授权Vectors with modified initiation codon for the translation of AAV-REP78 useful for production of AAV 有权具有用于AAV-REP78翻译的修饰起始密码子的载体可用于生产AAV公开(公告)号：US08952144B2
公开(公告)日：2015-02-10
申请号：US13945505
申请日：2013-07-18
申请人： uniQure IP B.V.
发明人： Wilhelmus Theodorus Johannes Maria Christiaan Hermens , Saskia Jacoba Petronella Haast , Dennis Johan Biesmans , Andrew Christian Bakker
IPC分类号： A61K39/12 , C12N15/86 , C07K14/005 , C12N7/00
CPC分类号： C12N15/86 , C07K14/005 , C12N7/00 , C12N2710/14043 , C12N2710/14044 , C12N2710/14143 , C12N2710/14144 , C12N2710/14152 , C12N2750/14121 , C12N2750/14122 , C12N2750/14143 , C12N2750/14151 , C12N2750/14152 , C12N2800/105 , C12N2800/50
摘要： The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AAV) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins.
摘要翻译： 本发明涉及用于在昆虫细胞中生产重组细小病毒（例如腺相关病毒）载体的核酸构建体，包含这种构建体的昆虫细胞以及其中细胞用于产生重组细小病毒病毒粒子的方法。 昆虫细胞优选包含编码细小病毒rep蛋白的第一核苷酸序列，由此翻译细小病毒Rep78蛋白的起始密码子是次要起始密码子，其在昆虫细胞中表达时会影响部分外显子跳跃。 昆虫细胞还包含包含至少一个细小病毒（AAV）倒置末端重复（ITR）核苷酸序列的第二核苷酸序列和包含编码细小病毒衣壳蛋白的序列的第三核苷酸序列。
-
3.发明授权Cultured silkworm cells capable of highly efficient baculovirus production and protein production 有权培养能够高效率的杆状病毒生产和蛋白质生产的蚕细胞公开(公告)号：US07955793B2
公开(公告)日：2011-06-07
申请号：US12226689
申请日：2007-04-26
申请人： Takahiro Kusakabe , Chisa Aoki , Osamu Ninagi , Jae Man Lee , Kazuhiro Iiyama , Naoya Kawakami
发明人： Takahiro Kusakabe , Chisa Aoki , Osamu Ninagi , Jae Man Lee , Kazuhiro Iiyama , Naoya Kawakami
IPC分类号： C12Q1/70
CPC分类号： C12P21/02 , C12N5/0601 , C12N7/00 , C12N2510/02 , C12N2710/14152
摘要： Known cell lines derived from silkworm exhibit low propagation efficiency of BmNPV. Accordingly, systems using known culture cell lines derived from silkworms take a long time to establish recombinant viruses, and are not suitable for preparation of virus solutions with high titers. The present invention provides a cell line Bme21 (FERM P-20852) that is derived from a silkworm embryo and is highly susceptible to BmNPV or its variant having the same biological characteristics. The present invention also provides a method of producing a recombinant virus, a method of producing a recombinant protein, a method of increasing efficiency of recombinant virus production, and a method of increasing efficiency of recombinant protein production, using the cell line Bme21 or its variant.
摘要翻译： 来自家蚕的已知细胞系表现出较低的BmNPV传播效率。 因此，使用来自蚕的已知培养细胞系的系统需要很长时间来建立重组病毒，并且不适于制备具有高滴度的病毒溶液。 本发明提供源自蚕胚的细胞系Bme21（FERM P-20852），并且对具有相同生物学特性的BmNPV或其变体高度敏感。 本发明还提供重组病毒的制造方法，重组蛋白质的制造方法，提高重组病毒生产效率的方法以及使用细胞系Bme21或其变体提高重组蛋白质生产效率的方法 。
-
4.发明申请公开(公告)号：US20180258448A1
公开(公告)日：2018-09-13
申请号：US15978669
申请日：2018-05-14
申请人： uniQure IP B.V.
发明人： Wilhelmus Theodorus Johannes Maria Christiaan HERMENS , Saskia Jacoba Petronella HAAST , Dennis Johan BIESMANS , Andrew Christian BAKKER
IPC分类号： C12N15/86 , C07K14/005 , C12N7/00
CPC分类号： C12N15/86 , C07K14/005 , C12N7/00 , C12N2710/14043 , C12N2710/14044 , C12N2710/14143 , C12N2710/14144 , C12N2710/14152 , C12N2750/14121 , C12N2750/14122 , C12N2750/14143 , C12N2750/14151 , C12N2750/14152 , C12N2800/105 , C12N2800/50
摘要： The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AA V) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins.
-
5.发明申请公开(公告)号：US20170145440A1
公开(公告)日：2017-05-25
申请号：US15424560
申请日：2017-02-03
申请人： uniQure IP B.V.
发明人： Wilhelmus Theodorus Johannes Maria Christiaan HERMENS , Saskia Jacoba Petronella HAAST , Dennis Johan BIESMANS , Andrew Christian BAKKER
CPC分类号： C12N15/86 , C07K14/005 , C12N7/00 , C12N2710/14043 , C12N2710/14044 , C12N2710/14143 , C12N2710/14144 , C12N2710/14152 , C12N2750/14121 , C12N2750/14122 , C12N2750/14143 , C12N2750/14151 , C12N2750/14152 , C12N2800/105 , C12N2800/50
摘要： The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AA V) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins.
-
6.发明申请BACULOVIRUS-BASED PRODUCTION OF BIOPHARMACEUTICALS FREE OF CONTAMINATING BACULOVIRAL VIRIONS 有权基于细菌病毒的生物制品不含污染细菌病毒公开(公告)号：US20150252335A1
公开(公告)日：2015-09-10
申请号：US14670459
申请日：2015-03-27
申请人： Genethon
CPC分类号： C12N7/00 , C12N15/86 , C12N2710/14143 , C12N2710/14152 , C12N2750/14143 , C12N2800/50
摘要： The present invention relates to methods for the production of biopharmaceuticals implementing a baculovirus-based system. These methods advantageously allow the production of biopharmaceuticals with a reduced number of or without contaminating baculoviral virions.
摘要翻译： 本发明涉及生产实施基于杆状病毒的系统的生物药物的方法。 这些方法有利地允许生产具有减少数量的或不含有污染性杆状病毒病毒粒子的生物药物。
-
7.发明申请BACULOVIRUS-BASED PRODUCTION OF BIOPHARMACEUTICALS FREE OF CONTAMINATING BACULOVIRAL VIRIONS 有权基于细菌病毒的生物制品不含污染细菌病毒公开(公告)号：US20120149010A1
公开(公告)日：2012-06-14
申请号：US13390806
申请日：2010-08-05
CPC分类号： C12N7/00 , C12N15/86 , C12N2710/14143 , C12N2710/14152 , C12N2750/14143 , C12N2800/50
摘要： The present invention relates to methods for the production of biopharmaceuticals implementing a baculovirus-based system. These methods advantageously allow the production of biopharmaceuticals with reduced or no contaminating baculoviral virions.
摘要翻译： 本发明涉及生产实施基于杆状病毒的系统的生物药物的方法。 这些方法有利地允许生产具有降低或不污染杆状病毒病毒粒子的生物药物。
-
8.发明申请公开(公告)号：US20190153473A1
公开(公告)日：2019-05-23
申请号：US16179648
申请日：2018-11-02
申请人： uniQure IP B.V.
发明人： Wilhelmus Theodorus Johannes Maria Christiaan HERMENS , Saskia Jacoba Petronella HAAST , Dennis Johan BIESMANS , Andrew Christian BAKKER
IPC分类号： C12N15/86 , C12N7/00 , C07K14/005
CPC分类号： C12N15/86 , C07K14/005 , C12N7/00 , C12N2710/14043 , C12N2710/14044 , C12N2710/14143 , C12N2710/14144 , C12N2710/14152 , C12N2750/14121 , C12N2750/14122 , C12N2750/14143 , C12N2750/14151 , C12N2750/14152 , C12N2800/105 , C12N2800/50
摘要： The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AAV) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins.
-
9.发明授权公开(公告)号：US09988645B2
公开(公告)日：2018-06-05
申请号：US15424560
申请日：2017-02-03
申请人： uniQure IP B.V.
发明人： Wilhelmus Theodorus Johannes Maria Christiaan Hermens , Saskia Jacoba Petronella Haast , Dennis Johan Biesmans , Andrew Christian Bakker
IPC分类号： C12N7/00 , C12N15/86 , C07K14/005 , C12N15/864 , C12N5/10
CPC分类号： C12N15/86 , C07K14/005 , C12N7/00 , C12N2710/14043 , C12N2710/14044 , C12N2710/14143 , C12N2710/14144 , C12N2710/14152 , C12N2750/14121 , C12N2750/14122 , C12N2750/14143 , C12N2750/14151 , C12N2750/14152 , C12N2800/105 , C12N2800/50
摘要： The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AA V) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins.
-
10.发明申请公开(公告)号：US20180127728A1
公开(公告)日：2018-05-10
申请号：US15841359
申请日：2017-12-14
申请人： GENETHON
CPC分类号： C12N7/00 , C12N15/86 , C12N2710/14143 , C12N2710/14152 , C12N2750/14143 , C12N2800/50
摘要： The present invention relates to methods for the production of biopharmaceuticals implementing a baculovirus-based system. These methods advantageously allow the production of biopharmaceuticals with a reduced number of or without contaminating baculoviral virions.
-
-
-
-
-
-
-
-
-
搜索结果
26 条记录 (耗时 0.019 秒)
