公开(公告)号：US20200056144A1

公开(公告)日：2020-02-20

申请号：US16499348

申请日：2018-03-30

申请人： UNIVERCELLS S.A.

发明人： José CASTILLO ,  Vasily MEDVEDEV

IPC分类号： C12M1/00 ,  C12M1/12 ,  C12M1/06 ,  C12M1/34 ,  B01J20/14 ,  C07K16/00 ,  B01D37/02 ,  B01D15/38

摘要： A method and a kit for cell culture clarification, as well as a system or apparatus for clarifying a cell culture and purifying biomolecules of interest, are described. The cell culture comprises at least one biomolecule of interest. The method comprises adding one or more compounds to the cell culture that allow the formation of floccules of solutes and/or particulates in said cell culture harvest, adding an amount of diatomaceous earth (DE) to said cell culture harvest, agitating the obtained solution, and transferring said solution to a filtration vessel comprising a support filter having a surface, whereby the diatomaceous earth in said solution forms a layer on said surface and filtering said harvest solution through said layer and support filter.

公开(公告)号：US20210095261A1

公开(公告)日：2021-04-01

申请号：US16499355

申请日：2018-03-30

申请人： UNIVERCELLS S.A.

发明人： José CASTILLO ,  Vasily MEDVEDEV

IPC分类号： C12N7/06 ,  C07K1/18

摘要： The invention provides a method and a kit for combined virus inactivation and capture/purification of a feed containing a protein of interest. The method comprises the steps of: contacting a feed comprising a protein of interest with negatively charged particles or media, conditioning the particles or media such that the pH is acidic thereby inactivating said virus, and eluting said protein of interest.

公开(公告)号：US20200318060A1

公开(公告)日：2020-10-08

申请号：US16649687

申请日：2018-09-27

申请人： UNIVERCELLS S.A.

发明人： José CASTILLO ,  Bastien Mairesse ,  Laetitia De Viron ,  Jean-Christophe Drugmand

IPC分类号： C12M1/00 ,  C12M1/26

摘要： The current disclosure concerns a system for producing biomolecules comprising a bioreactor including a chamber (1) suitable for receiving a liquid comprising cells and viral particles; and a concentrator (2), wherein said concentrator is equipped with a retentate conduit (300, 303) suitable for collecting said retentate and facilitating recirculating of the retentate to an input of said bioreactor or to an input of an intermediate vessel (4) positioned between said concentrator and said bioreactor. In a second and third aspect the disclosure concerns a method for producing biomolecules and the use of the disclosed system for the production of biomolecules.