公开(公告)号：US09611483B2

公开(公告)日：2017-04-04

申请号：US13761845

申请日：2013-02-07

申请人： University of Florida Research Foundation, Inc.

发明人： William O. Dawson ,  Svetlana Y. Folimonova

IPC分类号： C12N15/82 ,  C12N7/00 ,  C12N15/86

CPC分类号： C12N15/8203 ,  C12N7/00 ,  C12N15/86 ,  C12N2770/00034 ,  C12N2770/00043

摘要： Disclosed herein are viral vectors suitable for transfection into woody trees for purposes of delivering and expressing beneficial genes. Specifically exemplified herein are vectors for transfecting citrus trees. The vectors allow for the expression of useful proteins, such as those that can protect the tree from disease. Specifically exemplified herein are methods of transfecting woody trees that allow multiple applications of vectors while avoiding superinfection exclusion.

公开(公告)号：US20170298370A1

公开(公告)日：2017-10-19

申请号：US15477563

申请日：2017-04-03

申请人： University of Florida Research Foundation, Inc.

发明人： William O. Dawson ,  Svetlana Y. Folimonova

IPC分类号： C12N15/82 ,  C12N15/86 ,  C12N7/00 ,  A01H5/08

CPC分类号： C12N15/8203 ,  C12N7/00 ,  C12N15/86 ,  C12N2770/00034 ,  C12N2770/00043

摘要： Disclosed herein are viral vectors suitable for transfection into woody trees for purposes of delivering and expressing beneficial genes. Specifically exemplified herein are vectors for transfecting citrus trees. The vectors allow for the expression of useful proteins, such as those that can protect the tree from disease. Specifically exemplified herein are methods of transfecting woody trees that allow multiple applications of vectors while avoiding superinfection exclusion.

公开(公告)号：US10851381B2

公开(公告)日：2020-12-01

申请号：US16154576

申请日：2018-10-08

申请人： UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.

发明人： William O. Dawson ,  Svetlana Y. Folimonova ,  Alexey S. Folimonov

IPC分类号： C12N15/82

摘要： Disclosed herein are viral vectors based on modifications of the Citrus Tristeza virus useful for transfecting citrus trees for beneficial purposes. Included in the disclosure are viral vectors including one or more gene cassettes that encode heterologous polypeptide s. The gene cassettes are positioned at desirable locations on the viral genome so as to enable expression while preserving functionality of the virus. Also disclosed are methods of transfecting plants and plants transfected with viral vector embodiments.

公开(公告)号：US10472641B2

公开(公告)日：2019-11-12

申请号：US15477563

申请日：2017-04-03

申请人： University of Florida Research Foundation, Inc.

发明人： William O. Dawson ,  Svetlana Y. Folimonova

IPC分类号： C12N15/82 ,  C12N7/00 ,  C12N15/86

摘要： Disclosed herein are viral vectors suitable for transfection into woody trees for purposes of delivering and expressing beneficial genes. Specifically exemplified herein are vectors for transfecting citrus trees. The vectors allow for the expression of useful proteins, such as those that can protect the tree from disease. Specifically exemplified herein are methods of transfecting woody trees that allow multiple applications of vectors while avoiding superinfection exclusion.

公开(公告)号：US20130219556A1

公开(公告)日：2013-08-22

申请号：US13761845

申请日：2013-02-07

申请人： UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.

发明人： William O. Dawson ,  Svetlana Y. Folimonova

IPC分类号： C12N15/82

CPC分类号： C12N15/8203 ,  C12N7/00 ,  C12N15/86 ,  C12N2770/00034 ,  C12N2770/00043

摘要： Disclosed herein are viral vectors suitable for transfection into woody trees for purposes of delivering and expressing beneficial genes. Specifically exemplified herein are vectors for transfecting citrus trees. The vectors allow for the expression of useful proteins, such as those that can protect the tree from disease. Specifically exemplified herein are methods of transfecting woody trees that allow multiple applications of vectors while avoiding superinfection exclusion.

摘要翻译： 本文公开了适于转染木本树的病毒载体，用于递送和表达有益基因。 本文具体举例说明用于转染柑橘树的载体。 载体允许表达有用的蛋白质，例如可以保护树免于疾病的蛋白质。 具体举例说明的是转染木本树的方法，其允许多次施用载体同时避免超感染排除。