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    System for expression of genes in plants
    1.
    发明授权
    System for expression of genes in plants 有权

    公开(公告)号：US09765349B2

    公开(公告)日：2017-09-19

    申请号：US15398388

    申请日：2017-01-04

    申请人： iBio, Inc.

    发明人： Oleg Fedorkin ,  Shailaja Rabindran ,  Vidadi Yusibov

    IPC分类号： C12P21/02 ,  C12N15/82 ,  C12N7/00

    CPC分类号： C12N15/8203 ,  C07K14/61 ,  C07K14/62 ,  C12N7/00 ,  C12N15/8257 ,  C12N2770/00043 ,  C12P21/02

    摘要： The present invention provides trans-complementation systems for expressing gene products in plants. In general, the invention provides systems including a carrier vector and a producer vector, both based on plant viruses. The producer vector is defective for at least one function needed for successful systemic infection of a plant, e.g., replication, cell-to-cell movement, or long distance movement. The carrier vector supplies the missing function in trans. Certain producer vectors lack a functional coat protein coding sequence, in which case the corresponding producer vector supplies coat protein in trans. The invention also provides novel plant viral vectors and methods of use, e.g., to produce polypeptides or active RNAs in plants.

    System for expression of genes in plants
    2.
    发明授权
    System for expression of genes in plants 有权
    在植物中表达基因的系统

    公开(公告)号：US09551001B2

    公开(公告)日：2017-01-24

    申请号：US14602769

    申请日：2015-01-22

    申请人： iBio, Inc.

    发明人： Oleg Fedorkin ,  Shailaja Rabindran ,  Vidadi Yusibov

    IPC分类号： C12N15/82 ,  C07K14/61 ,  C07K14/62

    CPC分类号： C12N15/8203 ,  C07K14/61 ,  C07K14/62 ,  C12N7/00 ,  C12N15/8257 ,  C12N2770/00043 ,  C12P21/02

    摘要： The present invention provides trans-complementation systems for expressing gene products in plants. In general, the invention provides systems including a carrier vector and a producer vector, both based on plant viruses. The producer vector is defective for at least one function needed for successful systemic infection of a plant, e.g., replication, cell-to-cell movement, or long distance movement. The carrier vector supplies the missing function in trans. Certain producer vectors lack a functional coat protein coding sequence, in which case the corresponding producer vector supplies coat protein in trans. The invention also provides novel plant viral vectors and methods of use, e.g., to produce polypeptides or active RNAs in plants.

    摘要翻译： 本发明提供用于在植物中表达基因产物的反式互补系统。 通常，本发明提供了包括载体载体和生产者载体的系统，其都是基于植物病毒的。 生产者载体对于植物的成功的全身感染所需的至少一个功能是有缺陷的，例如复制，细胞到细胞运动或长距离运动。 载波向量以反向方式提供丢失的功能。 某些生产者载体缺乏功能外壳蛋白编码序列，在这种情况下，相应的生产者载体以反式提供外壳蛋白。 本发明还提供新的植物病毒载体和使用方法，例如在植物中产生多肽或活性RNA。

    RECOMBINANT ALSV FOR PREVENTING PATHOGENIC VIRAL INFECTION OF PLANT
    3.
    发明申请
    RECOMBINANT ALSV FOR PREVENTING PATHOGENIC VIRAL INFECTION OF PLANT 审中-公开
    用于预防植物病原体感染的重组ALSV

    公开(公告)号：US20150181887A1

    公开(公告)日：2015-07-02

    申请号：US14006767

    申请日：2011-05-13

    申请人： Nobuyuki Yoshikawa

    发明人： Nobuyuki Yoshikawa

    IPC分类号： A01N63/00

    CPC分类号： A01N63/00 ,  A01N25/00 ,  C12N15/8203 ,  C12N15/8283 ,  C12N2770/00043

    摘要： The invention provides a recombinant apple latent spherical virus (ALSV). The recombinant ALSV contains a partial fragment of a genomic RNA or cDNA from one or more plant pathogenic viruses, and manifests an interference effect against these plant pathogenic viruses. The invention thus provides a novel means of defending against infection of a plant pathogenic virus, without the risk of becoming virulence in the course of proliferation.

    摘要翻译： 本发明提供重组苹果潜球病毒（ALSV）。 重组ALSV含有来自一种或多种植物病原性病毒的基因组RNA或cDNA的部分片段，并表现出对这些植物病原性病毒的干扰作用。 因此，本发明提供了防止植物致病病毒感染的新方法，而不会在增殖过程中变成毒力的风险。

    Viral Based Transient-Expression Vector System That Allows Multiple Applications
    5.
    发明申请
    Viral Based Transient-Expression Vector System That Allows Multiple Applications 有权
    基于病毒的瞬态表达载体系统，允许多种应用

    公开(公告)号：US20130219556A1

    公开(公告)日：2013-08-22

    申请号：US13761845

    申请日：2013-02-07

    申请人： UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.

    发明人： William O. Dawson ,  Svetlana Y. Folimonova

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8203 ,  C12N7/00 ,  C12N15/86 ,  C12N2770/00034 ,  C12N2770/00043

    摘要： Disclosed herein are viral vectors suitable for transfection into woody trees for purposes of delivering and expressing beneficial genes. Specifically exemplified herein are vectors for transfecting citrus trees. The vectors allow for the expression of useful proteins, such as those that can protect the tree from disease. Specifically exemplified herein are methods of transfecting woody trees that allow multiple applications of vectors while avoiding superinfection exclusion.

    摘要翻译： 本文公开了适于转染木本树的病毒载体，用于递送和表达有益基因。 本文具体举例说明用于转染柑橘树的载体。 载体允许表达有用的蛋白质，例如可以保护树免于疾病的蛋白质。 具体举例说明的是转染木本树的方法，其允许多次施用载体同时避免超感染排除。

    System for expression of genes in plants
    6.
    发明授权
    System for expression of genes in plants 有权
    在植物中表达基因的系统

    公开(公告)号：US08058511B2

    公开(公告)日：2011-11-15

    申请号：US12035073

    申请日：2008-02-21

    申请人： Oleg Fedorkin ,  Shailaja Rabindran ,  Vidadi Yusibov

    发明人： Oleg Fedorkin ,  Shailaja Rabindran ,  Vidadi Yusibov

    IPC分类号： A01H1/06

    CPC分类号： C12N15/8203 ,  C07K14/61 ,  C07K14/62 ,  C12N7/00 ,  C12N15/8257 ,  C12N2770/00043 ,  C12P21/02

    摘要： The present invention provides trans-complementation systems for expressing gene products in plants. In general, the invention provides systems including a carrier vector and a producer vector, both based on plant viruses. The producer vector is defective for at least one function needed for successful systemic infection of a plant, e.g., replication, cell-to-cell movement, or long distance movement. The carrier vector supplies the missing function in trans. Certain producer vectors lack a functional coat protein coding sequence, in which case the corresponding producer vector supplies coat protein in trans. The invention also provides novel plant viral vectors and methods of use, e.g., to produce polypeptides or active RNAs in plants.

    摘要翻译： 本发明提供用于在植物中表达基因产物的反式互补系统。 通常，本发明提供了包括载体载体和生产者载体的系统，其都是基于植物病毒的。 生产者载体对于植物的成功的全身感染所需的至少一个功能是有缺陷的，例如复制，细胞到细胞运动或长距离运动。 载波向量以反向方式提供丢失的功能。 某些生产者载体缺乏功能外壳蛋白编码序列，在这种情况下，相应的生产者载体以反式提供外壳蛋白。 本发明还提供新的植物病毒载体和使用方法，例如在植物中产生多肽或活性RNA。