公开(公告)号：US20160231310A1

公开(公告)日：2016-08-11

申请号：US14915602

申请日：2014-09-02

申请人： UNIVERSITY OF MARYLAND, COLLEGE PARK ,  CHILDREN'S NATIONAL MEDICAL CENTER ,  THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPT., OF HEALTH AND HUMAN SERVICES

发明人： Omar Bilal AYYUB ,  Adam Michael BEHRENS ,  Peter KOFINAS ,  Marshall Lynn SUMMAR ,  Juan Manuel CABRERA-LUQUE ,  Gary CUNNINGHAM ,  Anton SIMEONOV ,  Juan MARUGAN

IPC分类号： G01N33/49 ,  G01N33/68 ,  G01N27/327

CPC分类号： G01N33/4925 ,  G01N21/78 ,  G01N27/327 ,  G01N27/3275 ,  G01N33/6893 ,  G01N33/84 ,  G01N2800/04

摘要： The present disclosure relates to a bio-sensor capable of measuring the total concentration of one or a plurality of ammonia or ammonium ions with the use of indophenol reagents in the presence of an ionomer. In some embodiments, the biosensor comprises a perflurinated membrane that comprises an ionomer in contact with an alkaline buffer in a vessel configured to receive a sample, such as whole blood. The disclosure also relates to a method of detecting or quantifying the ammonia or ammonium ion concentration in whole blood in a point of care biosensor without reliance on gas chromatography or any measurement that takes more than about twenty minutes.

摘要翻译： 本公开涉及能够在离聚物存在下使用靛酚试剂测量一种或多种氨或铵离子的总浓度的生物传感器。 在一些实施方案中，生物传感器包括含氟化膜，其包含离子交换体，其接触构造成接收诸如全血的样品的容器中的碱性缓冲液。 本公开还涉及在护理生物传感器的点处检测或定量全血中的氨或铵离子浓度的方法，而不依赖于气相色谱法或任何需要大约二十分钟的测量。

公开(公告)号：US20170198329A1

公开(公告)日：2017-07-13

申请号：US15304852

申请日：2015-04-17

申请人： UNIVERSITY OF MARYLAND, COLLEGE PARK ,  CHILDREN'S NATIONAL MEDICAL CENTER

发明人： Omar Bilal AYYUB ,  Brian Theodore HELIGMAN ,  Peter KOFINAS ,  Marshall Lynn SUMMAR ,  Juan Manuel CABRERA-LUQUE ,  Gary Cunningham

IPC分类号： C12Q1/00 ,  G01N33/68 ,  B01L3/00 ,  C12Q1/32

CPC分类号： C12Q1/005 ,  B01L3/502715 ,  B01L2200/10 ,  B01L2300/0887 ,  C12Q1/32 ,  G01N33/523 ,  G01N33/6812 ,  G01N33/6893 ,  G01N2333/902 ,  G01N2333/904 ,  G01N2333/90622 ,  G01N2800/04 ,  G01N2800/52

摘要： The present disclosure relates to a biosensor capable of measuring the total concentration of one or a plurality of amino acids with the use of a reaction surface comprising one or a plurality of metabolic enzymes or functional fragments thereof, but wherein the reaction surface does not comprise an electrode or electrically conductive support. In some embodiments, the biosensor comprises use of a thermophilic bacterial metabolic enzyme immobilized or attached to the reaction surface.