公开(公告)号：US10752884B2

公开(公告)日：2020-08-25

申请号：US15538465

申请日：2015-12-22

Applicant: UNIVERSITY OF SOUTH AUSTRALIA

Inventor： Yanchuang Han ,  Xin-Fu Zhou ,  WooKyung Kim ,  JunYong Yang ,  JiHee Kim

IPC: C12N5/071

Abstract: The disclosure relates to a method of producing induced beta cells from urine-derived cells, the method comprising providing urine-derived cells; inducing the urine-derived cells by culturing said urine-derived cells in a primary induction culture medium comprising an effective amount of at least one small molecule reprogramming factor(s) for a first period of time to obtain induced endoderm cells; inducing the induced endoderm cells by culturing said induced endoderm cells in a secondary induction culture medium comprising an effective amount of at least one small molecule reprogramming factor(s) for a second period of time to obtain induced pancreatic precursor cells; and inducing the induced pancreatic precursor cells by culturing said pancreatic precursor cells in a tertiary induction culture medium comprising an effective amount of at least one small molecule reprogramming factor(s) for a third period of time to obtain induced beta cells.