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公开(公告)号:US10752884B2
公开(公告)日:2020-08-25
申请号:US15538465
申请日:2015-12-22
Applicant: UNIVERSITY OF SOUTH AUSTRALIA
Inventor: Yanchuang Han , Xin-Fu Zhou , WooKyung Kim , JunYong Yang , JiHee Kim
IPC: C12N5/071
Abstract: The disclosure relates to a method of producing induced beta cells from urine-derived cells, the method comprising providing urine-derived cells; inducing the urine-derived cells by culturing said urine-derived cells in a primary induction culture medium comprising an effective amount of at least one small molecule reprogramming factor(s) for a first period of time to obtain induced endoderm cells; inducing the induced endoderm cells by culturing said induced endoderm cells in a secondary induction culture medium comprising an effective amount of at least one small molecule reprogramming factor(s) for a second period of time to obtain induced pancreatic precursor cells; and inducing the induced pancreatic precursor cells by culturing said pancreatic precursor cells in a tertiary induction culture medium comprising an effective amount of at least one small molecule reprogramming factor(s) for a third period of time to obtain induced beta cells.