公开(公告)号：US20150322405A1

公开(公告)日：2015-11-12

申请号：US14364200

申请日：2012-12-13

Applicant: FLINDERS UNIVERSITY OF SOUTH AUSTRALIA

Inventor： Yanchuang Han ,  Xin-Fu Zhou

IPC: C12N5/0797 ,  C12N5/0793 ,  C12N5/079

CPC classification number: C12N5/0623 ,  C12N5/0619 ,  C12N5/0622 ,  C12N2500/38 ,  C12N2501/065 ,  C12N2501/40 ,  C12N2501/72 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/1307 ,  G01N33/5073

Abstract: The present invention provides a method of producing a multipotent stem cell, said method comprising culturing at least one fibroblast cell in the presence of an effective amount of at least one small molecule reprogramming factor(s) that induces the cell to de-differentiate into a multipotent stem cell, wherein the method excludes the use of reprogramming factor(s) that are not small molecules. The small molecule reprogramming factor(s) may include a G9a HMTase inhibitor(s) and/or a MEK inhibitor(s) optionally in combination with other small molecule reprogramming factor(s). The invention also includes methods of differentiating the multipotent stem cells, cells produced by the methods, assays using the cells and kits for use in the methods.

Abstract translation: 本发明提供了一种产生多能干细胞的方法，所述方法包括在存在有效量的至少一种小分子重编程因子的情况下培养至少一种成纤维细胞，所述小分子重编程因子诱导细胞脱分化为 多能干细胞，其中该方法不包括使用不是小分子的重编程因子。 小分子重编程因子可以包括任选与其它小分子重编程因子组合的G9a HMTase抑制剂和/或MEK抑制剂。 本发明还包括区分多能干细胞，通过该方法产生的细胞，使用细胞的测定法和用于该方法的试剂盒的方法。

公开(公告)号：US10752884B2

公开(公告)日：2020-08-25

申请号：US15538465

申请日：2015-12-22

Applicant: UNIVERSITY OF SOUTH AUSTRALIA

Inventor： Yanchuang Han ,  Xin-Fu Zhou ,  WooKyung Kim ,  JunYong Yang ,  JiHee Kim

IPC: C12N5/071

Abstract: The disclosure relates to a method of producing induced beta cells from urine-derived cells, the method comprising providing urine-derived cells; inducing the urine-derived cells by culturing said urine-derived cells in a primary induction culture medium comprising an effective amount of at least one small molecule reprogramming factor(s) for a first period of time to obtain induced endoderm cells; inducing the induced endoderm cells by culturing said induced endoderm cells in a secondary induction culture medium comprising an effective amount of at least one small molecule reprogramming factor(s) for a second period of time to obtain induced pancreatic precursor cells; and inducing the induced pancreatic precursor cells by culturing said pancreatic precursor cells in a tertiary induction culture medium comprising an effective amount of at least one small molecule reprogramming factor(s) for a third period of time to obtain induced beta cells.

公开(公告)号：US10196606B2

公开(公告)日：2019-02-05

申请号：US14364200

申请日：2012-12-13

Applicant: FLINDERS UNIVERSITY OF SOUTH AUSTRALIA

Inventor： Xin-Fu Zhou ,  Yanchuang Han

IPC: C12N5/0797 ,  C12N5/079 ,  C12N5/0793 ,  G01N33/50

Abstract: The present invention provides a method of producing a multipotent stem cell, said method comprising culturing at least one fibroblast cell in the presence of an effective amount of at least one small molecule reprogramming factor(s) that induces the cell to de-differentiate into a multipotent stem cell, wherein the method excludes the use of reprogramming factor(s) that are not small molecules. The small molecule reprogramming factor(s) may include a G9a HMTase inhibitor(s) and/or a MEK inhibitor(s) optionally in combination with other small molecule reprogramming factor(s). The invention also includes methods of differentiating the multipotent stem cells, cells produced by the methods, assays using the cells and kits for use in the methods.