公开(公告)号：US20220119467A1

公开(公告)日：2022-04-21

申请号：US17419236

申请日：2020-01-06

Applicant: The Regents of the University of California ,  University of Washington

Inventor： David Baker ,  Scott Boyken ,  Hana El-Samad ,  Marc Lajoie ,  Robert Langan ,  Andrew Ng

IPC: C07K14/47 ,  A61K35/17

Abstract: Provided are molecular feedback circuits employing caged-degrons. Aspects of such circuits include the use of a caged-degron to modulate the output of a signaling pathway in a feedback-controlled manner. Also provided are nucleic acids encoding molecular circuits and cells containing such nucleic acids. Methods of using caged-degron-based molecular feedback circuits are also provided, including e.g., methods of modulating a signaling pathway of a cell that include genetically modifying the cell with a caged-degron-based molecular feedback circuit.

公开(公告)号：US10988514B2

公开(公告)日：2021-04-27

申请号：US16088686

申请日：2017-03-31

Applicant: UNIVERSITY OF WASHINGTON

Inventor： David Baker ,  Scott Boyken ,  Zibo Chen ,  Chunfu Xu ,  Sherry Bermeo ,  Robert Langan

IPC: C07K14/00 ,  C07K14/435 ,  C07K1/113 ,  G16B15/20 ,  C07K2/00 ,  C07K16/18

Abstract: Methods and apparatus for identifying and screening hydrogen bond networks are provided. A computing device can determine a search space for hydrogen bond networks related to one or more molecules, where the search space can include a plurality of energy terms related to a plurality of residues related to the hydrogen bond networks. The computing device can search the search space to identify one or more hydrogen bond networks based on the plurality of energy terms. The computing device can screen the identified hydrogen bond networks to identity one or more screened hydrogen bond networks based on scores for the identified hydrogen bond networks. An output can be generated that is related to the one or more screened hydrogen bond networks. Also provided are polypeptides that can form homo-oligomers with modular hydrogen bond network-mediated specificity.