公开(公告)号：US20190241849A1

公开(公告)日：2019-08-08

申请号：US16329779

申请日：2017-08-30

申请人： University of Kansas

发明人： Adam Joseph Mellott

IPC分类号： C12M1/32 ,  C12N5/00 ,  C12M3/00 ,  C12M1/12

CPC分类号： C12M23/12 ,  C08B37/003 ,  C08B37/0072 ,  C08B37/0084 ,  C12M21/08 ,  C12M23/04 ,  C12N5/0062 ,  C12N5/0068 ,  C12N2513/00 ,  C12N2535/00

摘要： Expandable cell culture substrates are provided that allow continual expansion of adherent stem cells without the need to passage cells, thereby preserving health and stem cell characteristics. The expandable cell culture substrates can include various three-dimensional structures having various coupling mechanisms that allow the structures to be joined, including where the structures are configured as interlocking blocks made from a variety of materials. The three-dimensional structures can include various coupling points, such as protrusions and opposing indentions, to allow for interlocking of the structures in the x-, y-, and z-axial directions. As a cell culture reaches a particular density, additional units can be added to increase the surface area of the expandable cell culture substrate.