公开(公告)号：US09487762B1

公开(公告)日：2016-11-08

申请号：US14050020

申请日：2013-10-09

申请人： University of Kentucky Research Foundation

发明人： Joe Chappell ,  Thomas D. Niehaus ,  Shigeru Okada ,  Timothy P. Devarenne ,  David S. Watt

IPC分类号： C12N9/10

CPC分类号： C12N9/1007 ,  C12N9/1085 ,  C12N15/8243 ,  C12Y201/01262 ,  C12Y205/0101

摘要： A method and system are provided for the production of triterpene including methylated triterpenes. The method and system include isolated nucleic acid sequences encoding triterpene methyltransferases such as triterpene methyltransferases 1, 2, 3. Advantageously, the method and system includes transgenic plant cells via an expression vector for triterpene methyltransferase and optionally various triterpene synthase and prenyltransferase all with tags directing these enzymes to the chloroplast of the transgenic plant cells for using the chloroplast methyl erythritol phosphate (MEP) pathway in the triterpene biogenesis.

摘要翻译： 提供了一种生产三萜（包括甲基化三萜烯）的方法和系统。 该方法和系统包括编码三萜甲基转移酶如三萜甲基转移酶1,2,3的分离的核酸序列。有利的是，该方法和系统包括通过三萜甲基转移酶表达载体和任选的各种三萜合酶和异戊烯转移酶的转基因植物细胞， 这些酶对转基因植物细胞的叶绿体使用叶绿体甲基赤藓糖磷酸酯（MEP）途径在三萜生物发生中。