公开(公告)号：US20230277579A1

公开(公告)日：2023-09-07

申请号：US18055525

申请日：2022-11-15

申请人： American Gene Technologies International Inc.

发明人： Charles David Pauza ,  Haishan Li ,  Tyler Lahusen ,  Mei-Ling Liou

IPC分类号： A61K31/7105 ,  A61P35/02 ,  A61K47/68 ,  A61K47/69 ,  A61K31/675 ,  C12N15/113 ,  C12N15/86

CPC分类号： A61K31/7105 ,  A61P35/02 ,  A61K47/6807 ,  A61K47/6901 ,  A61K31/675 ,  C12N15/1137 ,  C12N15/86 ,  C12Y205/0101 ,  A61K2300/00 ,  C12N2310/14 ,  C12N2310/531 ,  C12N2330/51 ,  C12N2740/16032 ,  C12N2740/16043 ,  C12N2740/16045 ,  C12N2750/14143 ,  C12N2810/6072

摘要： The present invention relates generally to methods and compositions for gene therapy and immunotherapy that activate gamma delta T-cells, and in particular, can be used in the treatment of various cancers and infectious diseases.

公开(公告)号：US20180142265A1

公开(公告)日：2018-05-24

申请号：US15867306

申请日：2018-01-10

申请人： LanzaTech New Zealand Limited

发明人： Wendy Yiting Chen ,  FungMin Liew ,  Michael Koepke

IPC分类号： C12P5/00 ,  C12N15/74 ,  C12P7/42 ,  C12N15/52 ,  C12P9/00

CPC分类号： C12P5/007 ,  C12N9/0006 ,  C12N9/1022 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/1085 ,  C12N9/1205 ,  C12N9/1229 ,  C12N9/88 ,  C12N15/52 ,  C12N15/74 ,  C12P7/42 ,  C12P9/00 ,  C12Y101/01088 ,  C12Y101/01267 ,  C12Y117/07001 ,  C12Y202/01007 ,  C12Y203/01009 ,  C12Y203/0301 ,  C12Y205/0101 ,  C12Y205/0109 ,  C12Y207/01036 ,  C12Y207/01148 ,  C12Y207/04002 ,  C12Y207/0706 ,  C12Y401/01033 ,  C12Y402/03027 ,  C12Y402/03046 ,  C12Y406/01012 ,  C12Y503/03002 ,  Y02E50/343

摘要： The invention provides a method for producing a terpene or a precursor thereof by microbial fermentation. Typically, the method involves culturing a recombinant bacterium in the presence of a gaseous substrate whereby the bacterium produces a terpene or a precursor thereof, such as mevalonic acid, isopentenyl pyrophosphate, dimethylallyl pyrophosphate, isoprene, geranyl pyrophosphate, farnesyl pyrophosphate, and/or farnesene. The bacterium may comprise one or more exogenous enzymes, such as enzymes in mevalonate, DXS, or terpene biosynthesis pathways.

公开(公告)号：US20180105838A1

公开(公告)日：2018-04-19

申请号：US15557370

申请日：2016-03-11

申请人： BASF SE

发明人： Jens SCHRADER ,  Markus BUCHHAUPT ,  Frank SONNTAG ,  Cora KRONER ,  Heike BRÜSER ,  Hartwig SCHRÖDER ,  Ralf PELZER

IPC分类号： C12P5/00 ,  C12N9/88 ,  C12N9/10 ,  C12N9/04 ,  C12N9/12 ,  C12N9/90

CPC分类号： C12P5/007 ,  C12N9/0006 ,  C12N9/1025 ,  C12N9/1085 ,  C12N9/1205 ,  C12N9/1229 ,  C12N9/88 ,  C12N9/90 ,  C12Y101/01034 ,  C12Y203/0301 ,  C12Y205/0101 ,  C12Y207/01036 ,  C12Y207/04002 ,  C12Y401/01 ,  C12Y401/01033 ,  C12Y402/03104 ,  C12Y503/03002

摘要： The invention relates to microbial terpene production. Known methods for microbial production of terpenes are mostly based on the direct conversion of sugars. Therefore alternative substrates, in particular alternative carbon sources, for use in microbial terpene production were desirable. The invention relates to a methylotrophic bacterium containing recombinant DNA coding for at least one polypeptide with enzymatic activity for heterologous expression in said bacterium, wherein said at least one polypeptide with enzymatic activity is selected from the group consisting an enzyme of a heterologous mevalonate pathway, a heterologous terpene synthase and optionally a heterologous synthase of a prenyl diphosphate precursor. The invention further relates in particular to a method for de novo microbial synthesis of sesquiterpenes or diterpenes from methanol and/or ethanol.

公开(公告)号：US20240229085A1

公开(公告)日：2024-07-11

申请号：US18393696

申请日：2023-12-22

申请人： NEOCANNBIO CO., LTD.

发明人： Jung Ho SONG ,  Jeong Kook KIM

IPC分类号： C12P7/42 ,  C12N1/20 ,  C12N9/10 ,  C12N15/70

CPC分类号： C12P7/42 ,  C12N1/20 ,  C12N9/1085 ,  C12N15/70 ,  C12N2501/999 ,  C12Y205/0101

摘要： The disclosure relates to a polypeptide including a genetic mutation encoding prenyltransferase, a recombinant vector, a recombinant microorganism for producing cannabigerolic acid and derivatives thereof, and a method of producing cannabigerolic acid and derivatives thereof, wherein the recombinant microorganism for producing cannabigerolic acid and derivatives thereof is transformed with the recombinant vector have increased biosynthetic reactivity, enabling stable mass production of cannabigerolic acid and derivatives thereof, and the method of producing cannabigerolic acid and derivatives thereof may produce various cannabinoids with high productivity and yield at a low production cost.

公开(公告)号：US11993635B2

公开(公告)日：2024-05-28

申请号：US17816937

申请日：2022-08-02

申请人： The Regents of the University of Colorado, a Body Corporate

发明人： Jerome Fox ,  Ankur Sarkar ,  Akarawin Hongdusit ,  Edward Kim

IPC分类号： C12N1/20 ,  C07K14/005 ,  C07K14/47

CPC分类号： C07K14/005 ,  C07K14/47 ,  C12N1/20 ,  C07K2319/80 ,  C12Y205/01001 ,  C12Y205/0101 ,  C12Y207/01036 ,  C12Y207/04002 ,  C12Y301/01048 ,  C12Y401/01033 ,  C12Y402/03017 ,  C12Y402/03018 ,  C12Y402/03024 ,  C12Y402/03056 ,  C12Y503/03002

摘要： This invention relates to the field of genetic engineering. Specifically, the invention relates to the construction of operons to produce biologically active agents. For example, operons may be constructed to produce agents that control the function of biochemical pathway proteins (e.g., protein phosphatases, kinases and/or proteases). Such agents may include inhibitors and modulators that may be used in studying or controlling phosphatase function associated with abnormalities in a phosphatase pathway or expression level. Fusion proteins, such as light activated protein phosphatases, may be genetically encoded and expressed as photoswitchable phosphatases. Systems are provided for use in controlling phosphatase function within living cells or in identifying small molecule inhibitors/activator/modulator molecules of protein phosphatases associated with cell signaling.

公开(公告)号：US20230183760A1

公开(公告)日：2023-06-15

申请号：US18172594

申请日：2023-02-22

申请人： MANUS BIO INC.

发明人： Ryan N. PHILIPPE ,  Ajikumar PARAYIL KUMARAN ,  Christine Nicole S. SANTOS ,  Jason DONALD ,  Stephen SARRIA

IPC分类号： C12P7/26 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88 ,  C12N15/52

CPC分类号： C12P7/26 ,  C12N9/0006 ,  C12N9/0071 ,  C12N9/1085 ,  C12N9/88 ,  C12N15/52 ,  C12Y205/0101 ,  C12Y402/03087

摘要： The present disclosure provides methods and compositions for producing rotundone. In various aspects, the present disclosure provides enzymes, polynucleotides encoding said enzymes, and recombinant microbial host cells (or microbial host strains) for the production of rotundone. In some embodiments, the present disclosure provides microbial host cells for producing rotundone at high purity and/or yield, from either enzymatic transformation of α-guaiene, or from sugar or other carbon source. The present disclosure further provides methods of making products containing rotundone, including flavor or fragrance products, among others.

公开(公告)号：US20170191103A1

公开(公告)日：2017-07-06

申请号：US15384420

申请日：2016-12-20

申请人： National University Corporation Chiba University

发明人： Daisuke Umeno ,  Maiko Furubayashi ,  Norihiko Misawa ,  Shinichi Takaichi

IPC分类号： C12P23/00 ,  C12N15/52

CPC分类号： C12P23/00 ,  C12N9/001 ,  C12N15/52 ,  C12P5/026 ,  C12Y103/99029 ,  C12Y205/0101 ,  C12Y205/01096 ,  Y02P20/52

摘要： The present invention provides a method of producing a carotenoid having 50 carbon atoms which comprises culturing, in a medium, a cell transformed with a mutant phytoene desaturase gene and obtaining the carotenoid having 50 carbon atoms from the culture. The mutant phytoene desaturase gene has an introduced mutation to encode a mutant phytoene desaturase having an enhanced activity to desaturate a carotenoid backbone compound of 50 carbon atoms.

公开(公告)号：US09487762B1

公开(公告)日：2016-11-08

申请号：US14050020

申请日：2013-10-09

申请人： University of Kentucky Research Foundation

发明人： Joe Chappell ,  Thomas D. Niehaus ,  Shigeru Okada ,  Timothy P. Devarenne ,  David S. Watt

IPC分类号： C12N9/10

CPC分类号： C12N9/1007 ,  C12N9/1085 ,  C12N15/8243 ,  C12Y201/01262 ,  C12Y205/0101

摘要： A method and system are provided for the production of triterpene including methylated triterpenes. The method and system include isolated nucleic acid sequences encoding triterpene methyltransferases such as triterpene methyltransferases 1, 2, 3. Advantageously, the method and system includes transgenic plant cells via an expression vector for triterpene methyltransferase and optionally various triterpene synthase and prenyltransferase all with tags directing these enzymes to the chloroplast of the transgenic plant cells for using the chloroplast methyl erythritol phosphate (MEP) pathway in the triterpene biogenesis.

摘要翻译： 提供了一种生产三萜（包括甲基化三萜烯）的方法和系统。 该方法和系统包括编码三萜甲基转移酶如三萜甲基转移酶1,2,3的分离的核酸序列。有利的是，该方法和系统包括通过三萜甲基转移酶表达载体和任选的各种三萜合酶和异戊烯转移酶的转基因植物细胞， 这些酶对转基因植物细胞的叶绿体使用叶绿体甲基赤藓糖磷酸酯（MEP）途径在三萜生物发生中。

公开(公告)号：US20160244775A1

公开(公告)日：2016-08-25

申请号：US15018181

申请日：2016-02-08

申请人： SUMITOMO RUBBER INDUSTRIES, LTD.

发明人： Yukino INOUE ,  Akari OKADA ,  Haruhiko YAMAGUCHI ,  Satoshi KURODA ,  Keiji TAKAGI

IPC分类号： C12N15/82 ,  C07K14/415 ,  C12N9/04 ,  C12N9/90 ,  C12N15/52 ,  C12N9/10

CPC分类号： C12N15/8257 ,  C07K14/415 ,  C12N9/0006 ,  C12N9/1085 ,  C12N9/90 ,  C12N15/52 ,  C12N15/8223 ,  C12N15/8243 ,  C12Y101/01034 ,  C12Y205/0101 ,  C12Y205/0102 ,  C12Y503/03002

摘要： Provided is a vector capable of improving polyisoprenoid production through the introduction of the vector into a plant using gene recombination techniques. A vector comprising: a promoter of a gene encoding MYC1 transcription factor; and a gene encoding a protein involved in polyisoprenoid biosynthesis, the gene being functionally linked to the promoter.

摘要翻译： 提供了能够通过使用基因重组技术将载体引入植物来改善聚异戊二烯生产的载体。 一种载体，其包含：编码MYC1转录因子的基因的启动子; 以及编码涉及聚异戊二烯生物合成的蛋白质的基因，该基因与启动子功能性连接。

公开(公告)号：US20150141714A1

公开(公告)日：2015-05-21

申请号：US14371765

申请日：2013-01-14

申请人： CHROMATIN, INC. ,  THE OHIO STATE UNIVERSITY

发明人： Joshua Blakeslee ,  Katrina Cornish ,  Oswald Crasta ,  Otto Folkerts ,  Dave Jessen ,  Ramesh Nair

IPC分类号： C12P5/00 ,  C07C11/21

CPC分类号： C12P5/007 ,  C07C11/21 ,  C12N15/8243 ,  C12P17/181 ,  C12Y101/01088 ,  C12Y202/01007 ,  C12Y205/0101 ,  C12Y402/03047 ,  Y02E50/343

摘要： The disclosed invention provides methods and compositions for increasing terpenoid production, such as sesquiterpenoids, such as farnesene, in plant cells.

摘要翻译： 所公开的发明提供了用于增加萜类化合物生产的方法和组合物，例如植物细胞中倍半萜类化合物，如法呢烯。