公开(公告)号：US20150218519A1

公开(公告)日：2015-08-06

申请号：US14591819

申请日：2015-01-07

申请人： University of Maryland, Baltimore ,  The United States as Represented by the Department of Veterans Affairs

发明人： G. David Trisler ,  Christopher T. Bever, JR. ,  James E. Goolsby ,  Bernard M. Pessac

IPC分类号： C12N5/0775 ,  A61K35/28

CPC分类号： C12N5/0662 ,  A61K35/28 ,  A61K2035/124 ,  C12N5/0647 ,  C12N2500/30 ,  C12N2500/44 ,  C12N2500/90 ,  C12N2501/125 ,  C12N2501/23

摘要： The present invention relates to methods and compositions concerning isolation of proliferating cells. In particular, the invention regards enrichment of stem cells in a mixture of stem cells and non-stem cells, wherein the non-stem cells may be differentiated cells. The invention exploits the non-adherent property of stem cells, as opposed to the adherent property of differentiating cells, by serially passaging the suspended cells in liquid media.

摘要翻译： 本发明涉及关于增殖细胞分离的方法和组合物。 特别地，本发明涉及干细胞在干细胞和非干细胞混合物中的富集，其中非干细胞可以是分化细胞。 通过将悬浮细胞连续传代在液体培养基中，本发明利用了干细胞的非贴壁性质，与分化细胞的粘附性质相反。