公开(公告)号：US06613530B1

公开(公告)日：2003-09-02

申请号：US08776188

申请日：1997-01-24

申请人： Ursula-Henrike Wienhues ,  Cornelia Kruse-Müller ,  Eva Höss ,  Elke Faatz ,  Beatus Ofenloch-Hähnle ,  Christoph Seidel ,  Michael Wiedmann

发明人： Ursula-Henrike Wienhues ,  Cornelia Kruse-Müller ,  Eva Höss ,  Elke Faatz ,  Beatus Ofenloch-Hähnle ,  Christoph Seidel ,  Michael Wiedmann

IPC分类号： G01N33576

CPC分类号： C07K14/005 ,  C12N2740/16122 ,  C12N2770/24222 ,  G01N33/533 ,  G01N33/54306 ,  G01N33/6878 ,  G01N33/92 ,  Y10S435/975 ,  Y10S436/811

摘要： The invention concerns a method for the immunological determination of a specific antibody in a sample liquid in which the sample liquid is incubated in the presence of a solid phase with two antigens directed against the antibody to be determined of which the first antigen carries at least one marker group and the second antigen is (a) bound to the solid phase or (b) is present in a form capable of binding to the solid phase and the antibody to be determined is detected by determining the marker group in the solid phase or/and in the liquid phase characterized in that at least one of the two antigens contains several epitope regions which react with the antibody to be determined.

公开(公告)号：US20100136519A1

公开(公告)日：2010-06-03

申请号：US12606586

申请日：2009-10-27

申请人： Ursula-Henrike Wienhues ,  Cornelia Kruse-Müller ,  Eva Höss ,  Elke Faatz ,  Beatus Ofenloch-Hähnle ,  Christoph Seidel ,  Michael Wiedmann

发明人： Ursula-Henrike Wienhues ,  Cornelia Kruse-Müller ,  Eva Höss ,  Elke Faatz ,  Beatus Ofenloch-Hähnle ,  Christoph Seidel ,  Michael Wiedmann

IPC分类号： C12Q1/70 ,  G01N33/554 ,  G01N33/567

CPC分类号： C07K14/005 ,  C12N2740/16122 ,  C12N2770/24222 ,  G01N33/533 ,  G01N33/54306 ,  G01N33/6878 ,  G01N33/92 ,  Y10S435/975 ,  Y10S436/811

摘要： The invention concerns a process for the immunological determination of a specific antibody in a fluid sample. The process involves incubating the fluid sample in the presence of a solid phase with two antigens directed against the antibody whose presence is to be determined; the first antigen carries at least one marker group, while the second is either (a) bonded to the solid phase or (b) present in a form in which it can bond with the solid phase, and betrays the presence of the antibody being sought by showing the presence of the marker group in the solid phase and/or in the fluid phase. The proposed process is characterised by the fact that at least one of the two antigens has several epitopic regions which react with the antibody to be determined.

摘要翻译： 本发明涉及流体样品中特异性抗体的免疫学测定方法。 该方法包括在固相存在下孵育流体样品，其中两种抗原针对要测定其存在的抗体; 第一个抗原携带至少一个标记基团，而第二个是（a）与固相结合，或（b）以与固相结合的形式存在，并且表示所寻求的抗体的存在 通过在固相和/或流体相中显示标记基团的存在。 所提出的方法的特征在于，两种抗原中的至少一种具有几个与要测定的抗体反应的表位区域。

公开(公告)号：US07629295B2

公开(公告)日：2009-12-08

申请号：US10613018

申请日：2003-07-07

申请人： Ursula-Henrike Wienhues ,  Cornelia Kruse-Müller ,  Eva Höss ,  Elke Faatz ,  Beatus Ofenloch-Hähnle ,  Christoph Seidel ,  Michael Wiedmann

发明人： Ursula-Henrike Wienhues ,  Cornelia Kruse-Müller ,  Eva Höss ,  Elke Faatz ,  Beatus Ofenloch-Hähnle ,  Christoph Seidel ,  Michael Wiedmann

IPC分类号： C40B30/04 ,  G01N33/53 ,  A61K38/00 ,  C12P21/08

CPC分类号： C07K14/005 ,  C12N2740/16122 ,  C12N2770/24222 ,  G01N33/533 ,  G01N33/54306 ,  G01N33/6878 ,  G01N33/92 ,  Y10S435/975 ,  Y10S436/811

摘要： The invention concerns a method for the immunological determination of a specific antibody in a sample liquid in which the sample liquid is incubated in the presence of a solid phase with two antigens directed against the antibody to be determined of which the first antigen carries at least one marker group and the second antigen is (a) bound to the solid phase or (b) is present in a form capable of binding to the solid phase and the antibody to be determined is detected by determining the marker group in the solid phase or/and in the liquid phase characterized in that at least one of the two antigens contains several epitope regions which react with the antibody to be determined.

摘要翻译： 本发明涉及一种用于免疫测定样品液体中的特异性抗体的方法，其中样品液体在固相存在下与针对待测定的抗体的两种抗原一起孵育，所述抗原决定于第一抗原携带至少一种 标记基团和第二抗原是（a）与固相结合或（b）以能够结合固相的形式存在，并且通过确定固相中的标记基团或/ 并且在液相中，其特征在于，两种抗原中的至少一种包含与要测定的抗体反应的几个表位区域。