公开(公告)号：US20120257037A1

公开(公告)日：2012-10-11

申请号：US13439954

申请日：2012-04-05

申请人： Valerica Raicu ,  Thomas John Mozer ,  Uwe Richard Muller

发明人： Valerica Raicu ,  Thomas John Mozer ,  Uwe Richard Muller

IPC分类号： H04N7/18 ,  G06K9/00

CPC分类号： G01J3/2803 ,  G01J3/027 ,  G01J3/0294 ,  G01J3/06 ,  G01J3/4406 ,  G01N15/1434 ,  G01N15/1463 ,  G01N15/1475 ,  G01N2015/1006 ,  G02B21/002 ,  G02B21/0076 ,  G02B21/16 ,  G02B27/0927 ,  G02B27/0983

摘要： A system and method of implementing a two-stage scanning technique with a high-speed microscope. The microscope is operable to provide spectrally resolved, multi-dimensional images from a single scan of a sample. The microscope may include one of a multi-beam point scanning microscope, a single beam line scanning microscope, and a multi-beam line scanning microscope. The sample is first tagged such that, if the sample has a particular makeup, it emits energy at particular wavelengths upon receiving excitation beams. The microscope is used to perform a first, wide area scan. If the sample is determined to have emitted energy having particular characteristics, the microscope performs a second, focused scan of the area that emitted the energy having the particular characteristics. The two-stage scanning technique is automated and may be used to quickly identify rare cells, microbes, viruses, and other components within one or more samples.

摘要翻译： 用高速显微镜实现两级扫描技术的系统和方法。 显微镜可操作以从样品的单次扫描提供光谱分辨的多维图像。 显微镜可以包括多光束点扫描显微镜，单束线扫描显微镜和多光束线扫描显微镜中的一个。 样品首先被标记，使得如果样品具有特定的组成，则在接收到激发光束时，其以特定波长发射能量。 显微镜用于执行第一次广域扫描。 如果确定样品具有具有特定特征的发射能量，则显微镜对发射具有特定特征的能量的区域进行第二次聚焦扫描。 两级扫描技术是自动化的，可用于快速识别一个或多个样品中的稀有细胞，微生物，病毒和其他成分。