公开(公告)号：US20120257196A1

公开(公告)日：2012-10-11

申请号：US13439951

申请日：2012-04-05

申请人： Valerica Raicu ,  Gabriel Biener

发明人： Valerica Raicu ,  Gabriel Biener

IPC分类号： G01J3/00

CPC分类号： G01J3/2803 ,  G01J3/027 ,  G01J3/0294 ,  G01J3/06 ,  G01J3/4406 ,  G01N15/1434 ,  G01N15/1463 ,  G01N15/1475 ,  G01N2015/1006 ,  G02B21/002 ,  G02B21/0076 ,  G02B21/16 ,  G02B27/0927 ,  G02B27/0983

摘要： A system and method of high-speed microscopy using a two-photon microscope with spectral resolution. The microscope is operable to provide two- to five-dimensional fluorescence images of samples, including two or three spatial dimensions, a spectral dimension (for fluorescence emission), and a temporal dimension (on a scale of less than approximately one second). Two-dimensional (spatial) images with a complete wavelength spectrum are generated from a single scan of a sample. The microscope may include one of a multi-beam point scanning microscope, a single beam line scanning microscope, and a multi-beam line scanning microscope. The line scans may be formed using one or more of curved mirrors and lenses. The multiple beams may be formed using one of a grating, an array of lenses, and a beam splitter.

摘要翻译： 使用具有光谱分辨率的双光子显微镜的高速显微镜的系统和方法。 显微镜可操作以提供样品的二维至五维荧光图像，包括两个或三个空间维度，光谱维度（用于荧光发射）和时间维度（小于约一秒的刻度）。 从样品的单次扫描产生具有完整波长谱的二维（空间）图像。 显微镜可以包括多光束点扫描显微镜，单束线扫描显微镜和多光束线扫描显微镜中的一个。 线扫描可以使用一个或多个弯曲镜和透镜来形成。 多个光束可以使用光栅，透镜阵列和分束器中的一个来形成。

公开(公告)号：US20110134232A1

公开(公告)日：2011-06-09

申请号：US13028803

申请日：2011-02-16

申请人： Valerica Raicu ,  Russell Fung

发明人： Valerica Raicu ,  Russell Fung

IPC分类号： H04N7/18

CPC分类号： G01J3/2823 ,  G01N21/6458 ,  G02B21/002 ,  G02B21/16 ,  G02B21/367

摘要： A microscope for generating an image of a sample, the microscope includes a light source for generating a pulsed light; an optical scanner receiving the pulsed light and transmitting the pulsed light to the sample to be imaged causing the sample to emit energy; a dispersive element that receives the emitted energy from the sample, disperses the energy into its spectral elements and transmits the spectrally dispersed energy; and a camera that generates a spectrally resolved image of the sample based on the spectrally dispersed energy from the dispersive element. Also described is a method of generating spectrally resolved images of the sample.

摘要翻译： 用于产生样品图像的显微镜，所述显微镜包括用于产生脉冲光的光源; 光学扫描器接收脉冲光并将脉冲光传输到要成像的样品，使样品发射能量; 从样品接收发射的能量的分散元件将能量分散到其光谱元素中并透射光谱分散的能量; 以及相机，其基于来自色散元件的光谱分散的能量产生样品的光谱分辨图像。 还描述了产生样品的光谱分辨图像的方法。

公开(公告)号：US09103721B2

公开(公告)日：2015-08-11

申请号：US13439951

申请日：2012-04-05

申请人： Valerica Raicu ,  Gabriel Biener

发明人： Valerica Raicu ,  Gabriel Biener

IPC分类号： G01J3/00 ,  G01J3/28 ,  G02B21/00 ,  G01J3/02 ,  G01J3/06 ,  G01J3/44 ,  G02B21/16 ,  G02B27/09

CPC分类号： G01J3/2803 ,  G01J3/027 ,  G01J3/0294 ,  G01J3/06 ,  G01J3/4406 ,  G01N15/1434 ,  G01N15/1463 ,  G01N15/1475 ,  G01N2015/1006 ,  G02B21/002 ,  G02B21/0076 ,  G02B21/16 ,  G02B27/0927 ,  G02B27/0983

摘要： A system and method of high-speed microscopy using a two-photon microscope with spectral resolution. The microscope is operable to provide two- to five-dimensional fluorescence images of samples, including two or three spatial dimensions, a spectral dimension (for fluorescence emission), and a temporal dimension (on a scale of less than approximately one second). Two-dimensional (spatial) images with a complete wavelength spectrum are generated from a single scan of a sample. The microscope may include one of a multi-beam point scanning microscope, a single beam line scanning microscope, and a multi-beam line scanning microscope. The line scans may be formed using one or more of curved mirrors and lenses. The multiple beams may be formed using one of a grating, an array of lenses, and a beam splitter.

摘要翻译： 使用具有光谱分辨率的双光子显微镜的高速显微镜的系统和方法。 显微镜可操作以提供样品的二维至五维荧光图像，包括两个或三个空间维度，光谱维度（用于荧光发射）和时间维度（小于约一秒的刻度）。 从样品的单次扫描产生具有完整波长谱的二维（空间）图像。 显微镜可以包括多光束点扫描显微镜，单束线扫描显微镜和多光束线扫描显微镜中的一个。 线扫描可以使用一个或多个弯曲镜和透镜来形成。 多个光束可以使用光栅，透镜阵列和分束器中的一个来形成。

公开(公告)号：US07973927B2

公开(公告)日：2011-07-05

申请号：US11904860

申请日：2007-09-28

申请人： Valerica Raicu ,  Russell Fung

发明人： Valerica Raicu ,  Russell Fung

IPC分类号： G01J3/30

CPC分类号： G01J3/2823 ,  G01N21/6458 ,  G02B21/002 ,  G02B21/16 ,  G02B21/367

摘要： A microscope for generating an image of a sample, the microscope includes a light source for generating a pulsed light; a scanning mirror receiving the pulsed light and transmitting the pulsed light to the sample to be imaged causing the sample to emit energy; a dispersive element that receives the emitted energy from the sample, disperses the energy into its spectral elements and transmits the spectrally dispersed energy; and a camera that generates a spectrally resolved image of the sample based on the spectrally dispersed energy from the dispersive element. Also described is a method of generating spectrally resolved images of the sample.

摘要翻译： 用于产生样品图像的显微镜，所述显微镜包括用于产生脉冲光的光源; 接收脉冲光并将脉冲光传输到要成像的样品使得样品发射能量的扫描镜; 从样品接收发射的能量的分散元件将能量分散到其光谱元素中并透射光谱分散的能量; 以及相机，其基于来自色散元件的光谱分散的能量产生样品的光谱分辨图像。 还描述了产生样品的光谱分辨图像的方法。

公开(公告)号：US08982206B2

公开(公告)日：2015-03-17

申请号：US13439958

申请日：2012-04-05

申请人： Valerica Raicu ,  Michael Stoneman

发明人： Valerica Raicu ,  Michael Stoneman

IPC分类号： H04N7/18 ,  G01J3/28 ,  G02B21/00 ,  G01J3/02 ,  G01J3/06 ,  G01J3/44 ,  G02B21/16 ,  G02B27/09

CPC分类号： G01J3/2803 ,  G01J3/027 ,  G01J3/0294 ,  G01J3/06 ,  G01J3/4406 ,  G01N15/1434 ,  G01N15/1463 ,  G01N15/1475 ,  G01N2015/1006 ,  G02B21/002 ,  G02B21/0076 ,  G02B21/16 ,  G02B27/0927 ,  G02B27/0983

摘要： A system and method of high-speed microscopy using a two-photon microscope with spectral resolution. The microscope is operable to provide spectrally resolved, multi-dimensional images from a single scan of a sample. The microscope may include one of a multi-beam point scanning microscope, a single beam line scanning microscope, and a multi-beam line scanning microscope. The microscope includes a descanning arrangement such that emitted fluorescence is static on a receiving detector. The detector is a narrow detector with a width at least half the size of the length, to reduce the amount of pixel data being transmitted and improve scan speeds. The microscope may also incorporate one or more binning techniques whereby pixels are binned together to improve resolution or scan speeds.

摘要翻译： 使用具有光谱分辨率的双光子显微镜的高速显微镜的系统和方法。 显微镜可操作以从样品的单次扫描提供光谱分辨的多维图像。 显微镜可以包括多光束点扫描显微镜，单束线扫描显微镜和多光束线扫描显微镜中的一个。 显微镜包括一种下拉排列，使得发射的荧光在接收检测器上是静态的。 检测器是窄度检测器，其宽度至少为长度的一半，以减少被传输的像素数据量并提高扫描速度。 显微镜还可以包含一个或多个分档技术，由此将像素合并在一起以提高分辨率或扫描速度。

公开(公告)号：US20120257038A1

公开(公告)日：2012-10-11

申请号：US13439958

申请日：2012-04-05

申请人： Valerica Raicu ,  Michael Stoneman

发明人： Valerica Raicu ,  Michael Stoneman

IPC分类号： H04N7/18 ,  G01J3/42

CPC分类号： G01J3/2803 ,  G01J3/027 ,  G01J3/0294 ,  G01J3/06 ,  G01J3/4406 ,  G01N15/1434 ,  G01N15/1463 ,  G01N15/1475 ,  G01N2015/1006 ,  G02B21/002 ,  G02B21/0076 ,  G02B21/16 ,  G02B27/0927 ,  G02B27/0983

摘要： A system and method of high-speed microscopy using a two-photon microscope with spectral resolution. The microscope is operable to provide spectrally resolved, multi-dimensional images from a single scan of a sample. The microscope may include one of a multi-beam point scanning microscope, a single beam line scanning microscope, and a multi-beam line scanning microscope. The microscope includes a descanning arrangement such that emitted fluorescence is static on a receiving detector. The detector is a narrow detector with a width at least half the size of the length, to reduce the amount of pixel data being transmitted and improve scan speeds. The microscope may also incorporate one or more binning techniques whereby pixels are binned together to improve resolution or scan speeds.

摘要翻译： 使用具有光谱分辨率的双光子显微镜的高速显微镜的系统和方法。 显微镜可操作以从样品的单次扫描提供光谱分辨的多维图像。 显微镜可以包括多光束点扫描显微镜，单束线扫描显微镜和多光束线扫描显微镜中的一个。 显微镜包括一种下拉排列，使得发射的荧光在接收检测器上是静态的。 检测器是窄度检测器，其宽度至少为长度的一半，以减少被传输的像素数据量并提高扫描速度。 显微镜还可以包含一个或多个分档技术，由此将像素合并在一起以提高分辨率或扫描速度。

公开(公告)号：US08094304B2

公开(公告)日：2012-01-10

申请号：US13028803

申请日：2011-02-16

申请人： Valerica Raicu ,  Russell Fung

发明人： Valerica Raicu ,  Russell Fung

IPC分类号： G01J3/30

CPC分类号： G01J3/2823 ,  G01N21/6458 ,  G02B21/002 ,  G02B21/16 ,  G02B21/367

摘要： A microscope for generating an image of a sample, the microscope includes a light source for generating a pulsed light; an optical scanner receiving the pulsed light and transmitting the pulsed light to the sample to be imaged causing the sample to emit energy; a dispersive element that receives the emitted energy from the sample, disperses the energy into its spectral elements and transmits the spectrally dispersed energy; and a camera that generates a spectrally resolved image of the sample based on the spectrally dispersed energy from the dispersive element. Also described is a method of generating spectrally resolved images of the sample.

摘要翻译： 用于产生样品图像的显微镜，所述显微镜包括用于产生脉冲光的光源; 光学扫描器接收脉冲光并将脉冲光传输到要成像的样品，使样品发射能量; 从样品接收发射的能量的分散元件将能量分散到其光谱元素中并透射光谱分散的能量; 以及相机，其基于来自色散元件的光谱分散的能量产生样品的光谱分辨图像。 还描述了产生样品的光谱分辨图像的方法。

公开(公告)号：US20120257037A1

公开(公告)日：2012-10-11

申请号：US13439954

申请日：2012-04-05

申请人： Valerica Raicu ,  Thomas John Mozer ,  Uwe Richard Muller

发明人： Valerica Raicu ,  Thomas John Mozer ,  Uwe Richard Muller

IPC分类号： H04N7/18 ,  G06K9/00

CPC分类号： G01J3/2803 ,  G01J3/027 ,  G01J3/0294 ,  G01J3/06 ,  G01J3/4406 ,  G01N15/1434 ,  G01N15/1463 ,  G01N15/1475 ,  G01N2015/1006 ,  G02B21/002 ,  G02B21/0076 ,  G02B21/16 ,  G02B27/0927 ,  G02B27/0983

摘要： A system and method of implementing a two-stage scanning technique with a high-speed microscope. The microscope is operable to provide spectrally resolved, multi-dimensional images from a single scan of a sample. The microscope may include one of a multi-beam point scanning microscope, a single beam line scanning microscope, and a multi-beam line scanning microscope. The sample is first tagged such that, if the sample has a particular makeup, it emits energy at particular wavelengths upon receiving excitation beams. The microscope is used to perform a first, wide area scan. If the sample is determined to have emitted energy having particular characteristics, the microscope performs a second, focused scan of the area that emitted the energy having the particular characteristics. The two-stage scanning technique is automated and may be used to quickly identify rare cells, microbes, viruses, and other components within one or more samples.

摘要翻译： 用高速显微镜实现两级扫描技术的系统和方法。 显微镜可操作以从样品的单次扫描提供光谱分辨的多维图像。 显微镜可以包括多光束点扫描显微镜，单束线扫描显微镜和多光束线扫描显微镜中的一个。 样品首先被标记，使得如果样品具有特定的组成，则在接收到激发光束时，其以特定波长发射能量。 显微镜用于执行第一次广域扫描。 如果确定样品具有具有特定特征的发射能量，则显微镜对发射具有特定特征的能量的区域进行第二次聚焦扫描。 两级扫描技术是自动化的，可用于快速识别一个或多个样品中的稀有细胞，微生物，病毒和其他成分。

公开(公告)号：US20080088838A1

公开(公告)日：2008-04-17

申请号：US11904860

申请日：2007-09-28

申请人： Valerica Raicu ,  Russell Fung

发明人： Valerica Raicu ,  Russell Fung

IPC分类号： G01J3/30

CPC分类号： G01J3/2823 ,  G01N21/6458 ,  G02B21/002 ,  G02B21/16 ,  G02B21/367

摘要： A microscope for generating an image of a sample, the microscope includes a light source for generating a pulsed light; a scanning mirror receiving the pulsed light and transmitting the pulsed light to the sample to be imaged causing the sample to emit energy; a dispersive element that receives the emitted energy from the sample, disburses the energy into its spectral elements and transmits the spectrally disbursed energy; and a camera that generates a spectrally resolved image of the sample based on the spectrally disbursed energy from the dispersive element. Also described is a method of generating spectrally resolved images of the sample.

摘要翻译： 用于产生样品图像的显微镜，所述显微镜包括用于产生脉冲光的光源; 接收脉冲光并将脉冲光传输到要成像的样品使得样品发射能量的扫描镜; 从样品接收发射的能量的分散元件，将能量散发到其光谱元素中并传输光谱发射的能量; 以及相机，其基于来自色散元件的光谱分布的能量产生样品的光谱分辨图像。 还描述了产生样品的光谱分辨图像的方法。