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    Luminescent lanthanide chelates with decreased non-radiative energy loss
    1.
    发明授权
    Luminescent lanthanide chelates with decreased non-radiative energy loss 失效
    发光镧系元素螯合物具有降低的非辐射能量损失

    公开(公告)号：US5457186A

    公开(公告)日：1995-10-10

    申请号：US135525

    申请日：1993-10-13

    申请人： Veli-Matti Mukkala ,  Harri Takalo ,  Ilkka Hemmila ,  Jouko Kankare

    发明人： Veli-Matti Mukkala ,  Harri Takalo ,  Ilkka Hemmila ,  Jouko Kankare

    IPC分类号： G01N33/533 ,  C07F5/00 ,  C09K11/06

    CPC分类号： G01N33/533

    摘要： A method of conducting assays for analytes, usually from biological samples, utilizing bioaffinity reagents linked to luminescent lanthanide chelates, which are synthesized so that the non-radiative quenching of the ion luminescence through C--H bond vibrational energy manifolds is avoided using a stable chelate where the CH and CH.sub.2 groups in the vicinity of the emittive ion are substituted with CD and CD.sub.2 groups hence producing improved luminescence quantum yield and higher assay sensitivities.

    摘要翻译： 一种通过利用与发光镧系元素螯合物连接的生物亲和试剂进行分析物测定的方法，通常使用生物样品进行测定，所述生物亲和试剂被合成，以便避免使用稳定的螯合物通过CH键振动能量歧管进行离子发光的非辐射猝灭 在发射离子附近的CH和CH 2基团被CD和CD 2基团取代，从而产生改善的发光量子产率和更高的测定灵敏度。

    Metal-chelating 2,6-disubstituted pyridine compounds and their use
    4.
    发明授权
    Metal-chelating 2,6-disubstituted pyridine compounds and their use 失效
    金属螯合2,6-二取代的吡啶化合物及其用途

    公开(公告)号：US6127529A

    公开(公告)日：2000-10-03

    申请号：US759437

    申请日：1991-09-13

    申请人： Marek Kwiatkowski ,  Christian Sund ,  Jyrki Ylikoski ,  Veli-Matti Mukkala ,  Ilkka Hemmila

    发明人： Marek Kwiatkowski ,  Christian Sund ,  Jyrki Ylikoski ,  Veli-Matti Mukkala ,  Ilkka Hemmila

    IPC分类号： A61K49/06 ,  C07D213/30 ,  C07D213/38 ,  C07D213/56 ,  C07D213/89 ,  C07F9/58 ,  C07F15/00 ,  C07C245/00 ,  C07D401/06

    CPC分类号： C07D213/30 ,  A61K49/06 ,  C07D213/38 ,  C07D213/56 ,  C07D213/89 ,  C07F9/582 ,  C07F9/588

    摘要： Bifuncitonal chelating pyridine compound and its use for conferring chelating properties on organic compounds. The pyridine compound has the structure ##STR1## where (i) n is an integer 1 or 2,(ii) R.sub.1, R.sub.2 and R.sub.3 represent groups that have no electrons capable of significantly delocalizing or resonating with the pyridine ring, such as hydrogen, alkyl or aralkyl having an aliphatic carbon atom next to the pyridine ring; at least two of R.sub.1, R.sub.2 and R.sub.3 being hydrogen,iii) Z an Z' represent identical or different structures, each of which comprises at least one heteroatom having a free pair of electrons as that the said at least one heteroatom together with the nitrogen atom of the pyridine ring is capable of chelating a metal ion,iv) - - - - specifies that the group X-Y is a substituent replacing a hydrogen anywhere in the parent pyridine compound, andv) X-Y represents an organic group which is inert to said chelation, and in which iX is an inert and stable bridge and Y is a functional group or a residue of an organic compounbd that has properties conferred on the compound of formula II; said group X-Y being linked to the pyridine ring of formula II via an aliphatic carbon atom attached to and acid ester, salt and chelate forms thereof involving at least one of said chelating heteroatoms.

    摘要翻译： 双官能螯合吡啶化合物及其在有机化合物上赋予螯合性质的用途。 吡啶化合物具有其中（i）n为整数1或2的结构，（ii）R 1，R 2和R 3表示不具有能够与吡啶环显着离域或共振的电子的基团，例如氢，烷基或芳烷基 在吡啶环旁边具有脂肪族碳原子; R1，R2和R3中的至少两个是氢，iii）Z，Z'代表相同或不同的结构，每个结构包含至少一个具有自由对电子的杂原子，因为所述至少一个杂原子与氮 吡啶环的原子能够螯合金属离子，iv） - - - - 指定基团XY是取代母体吡啶化合物任何地方的氢的取代基，以及v）XY表示对所述吡啶化合物是惰性的有机基团 螯合，其中iX是惰性和稳定的桥，Y是具有赋予式II化合物的性质的有机化合物的官能团或残基; 所述基团X-Y通过连接到其上的脂族碳原子和与其所述螯合杂原子中至少一个的酸酯，其盐和螯合物形式连接在式II的吡啶环上。

    Fluorescence spectroscopy assay means with fluorescent chelate of a lanthanide
    6.
    发明授权
    Fluorescence spectroscopy assay means with fluorescent chelate of a lanthanide 失效
    荧光光谱测定法表示镧系元素的荧光螯合物

    公开(公告)号：US4374120A

    公开(公告)日：1983-02-15

    申请号：US128621

    申请日：1980-03-07

    申请人： Erkki Soini ,  Ilkka Hemmila

    发明人： Erkki Soini ,  Ilkka Hemmila

    IPC分类号： G01N21/64 ,  G01N33/58 ,  G01N21/33 ,  G01N33/48 ,  G01N33/52

    CPC分类号： G01N33/582 ,  G01N21/6408 ,  Y10S436/80

    摘要： An improved method of determining the nature of a substance by fluoroscence spectroscopy wherein a fluorescent marker is coupled to the molecules of the substance comprises the use of a marker having a longer period of fluorescence than those of possible sources of noise and by employing an exciting radiation pulse of short duration so that the fluorescence of the marker is detected after the objectionable sources of fluorescence have ceased; the marker including a fluorescent lanthanide chelate complex.

    摘要翻译： 通过荧光光谱法测定物质性质的改进方法，其中荧光标记与物质的分子偶联，包括使用具有比可能的噪声源更长的荧光周期的标记，并且通过采用激发辐射 脉冲持续时间短，使荧光标志物的荧光被检测到有害的荧光源已经停止; 标记物包括荧光镧系元素螯合物。

    Modulated homogeneous fluorescence biospecific affinity assay employing fluorescing lanthanide chelates as covalent labels
    7.
    发明授权
    Modulated homogeneous fluorescence biospecific affinity assay employing fluorescing lanthanide chelates as covalent labels 失效
    使用荧光镧系螯合物作为共价标签进行调制的均相荧光生物特异性亲和力测定

    公开(公告)号：US5637509A

    公开(公告)日：1997-06-10

    申请号：US626528

    申请日：1990-12-12

    申请人： Ilkka Hemmila ,  Timo Lovgren

    发明人： Ilkka Hemmila ,  Timo Lovgren

    IPC分类号： G01N33/533 ,  G01N33/542 ,  G01N33/58 ,  G01N33/53 ,  G01N33/536

    CPC分类号： G01N33/542 ,  G01N33/582 ,  G01N2458/40 ,  Y10S436/826

    摘要： Method for a homogeneous biospecific affinity assay for determining the content of a substance (analyte) in a biological sample. The assay is carried out in an aqueous reaction medium by means of time-resolved fluorescence spectroscopy and with a biospecific affinity reactant labeled with a lanthanide chelate in which a lanthanide ion exhibiting ionic fluorescence is chelated by a ligand bound covalently to the reactant. The characteristic feature is(i) that the lanthanide chelate formed by the lanthanide ion together with the covalently bound ligand forms a fluorescent chelate, and(ii) that a modulator is added which stabilizes the lanthanide chelate so that the lanthanide fluorescence as measured from the medium becomes a practically pure function of the analyte concentration therein.

    摘要翻译： 用于确定生物样品中物质（分析物）含量的均相生物特异性亲和测定法。 通过时间分辨荧光光谱法和用镧系元素螯合物标记的生物特异性亲和反应物在水性反应介质中进行测定，其中显示离子荧光的镧系元素离子通过与反应物共价键合的配体螯合。 其特征是（i）由镧系元素离子与共价结合的配体一起形成的镧系元素螯合物形成荧光螯合物，和（ii）添加稳定镧系元素螯合物的调节剂，使得镧系元素荧光从 培养基成为其中分析物浓度的实际纯度函数。