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    Germination-specific plant promoters
    2.
    发明授权
    Germination-specific plant promoters 失效
    萌发特异性植物启动子

    公开(公告)号：US06359196B1

    公开(公告)日：2002-03-19

    申请号：US09404390

    申请日：1999-09-23

    申请人： Finn Lok ,  Ole Olsen ,  Per-Johan Meijer ,  Verena Cameron-Mills

    发明人： Finn Lok ,  Ole Olsen ,  Per-Johan Meijer ,  Verena Cameron-Mills

    IPC分类号： A01H100

    CPC分类号： C12Y302/01031 ,  C07K14/8139 ,  C12N9/2408 ,  C12N15/8234 ,  C12N15/8238 ,  C12N15/8242 ,  C12Y302/0102 ,  C12Y302/01073

    摘要： Tissue-specific regulatory sequences, including promoters from barley (Hordeum vulgare) genes encoding &agr;-glucosidase and cystatin-1 (cysteine protease inhibitor) have been identified and isolated. The gene promoters have particular utility in directing high level, germination preferred expression of heterologous proteins that impart increased agronomic and malting characteristics to a given plant. In addition, the invention relates to methods of producing transgenic plants by using chimeric genes, cassette vectors, kits, cells and methods comprising the promoter and signal sequence coding region of the gene for barley &agr;-glucosidase and the gene for barley cystatin-1.

    摘要翻译： 已经鉴定并分离了组织特异性调节序列，包括来自编码α-葡糖苷酶和半胱氨酸蛋白酶抑制剂（半胱氨酸蛋白酶抑制剂）的大麦（Hordeum vulgare）基因的启动子。 基因启动子在指导异源蛋白质的高水平发芽优选表达方面具有特别的用途，赋予给定植物更多的农艺和麦芽特征。 此外，本发明涉及通过使用嵌合基因，盒载体，试剂盒，细胞和包含大麦α-葡糖苷酶基因的启动子和信号序列编码区和大麦半胱氨酸蛋白酶抑制蛋白-1基因的方法来生产转基因植物的方法。

    Barley with reduced lipoxygenase activity and beverage prepared therefrom
    6.
    发明授权
    Barley with reduced lipoxygenase activity and beverage prepared therefrom 有权
    具有降低的脂氧合酶活性的大麦和由其制备的饮料

    公开(公告)号：US09363959B2

    公开(公告)日：2016-06-14

    申请号：US13141579

    申请日：2009-12-28

    申请人： Birgitte Skadhauge ,  Finn Lok ,  Klaus Breddam ,  Ole Olsen ,  Lette Mølskov Bech ,  Søren Knudsen

    发明人： Birgitte Skadhauge ,  Finn Lok ,  Klaus Breddam ,  Ole Olsen ,  Lette Mølskov Bech ,  Søren Knudsen

    IPC分类号： A01H5/10 ,  C12C1/18 ,  C12C12/00

    CPC分类号： A01H5/10 ,  C12C1/18 ,  C12C12/00 ,  C12C2200/01

    摘要： According to the invention, there is provided barley with total loss of functional lipoxygenase (LOX)-1 and LOX-2 enzymes, and plant products produced thereof, such as malt manufactured by using barley kernels defective in the synthesis of the fatty acid-dioxygenating enzymes LOX-1 and LOX-2. Said enzymes account for the principal activities related to dioxygenation of linoleic acid into 9- and 13-hydroperoxy octadecadienoic acid, respectively. 9-Hydroperoxy octadecadienoic acid represents a LOX pathway metabolite, which—through further enzymatic or spontaneous reactions—may lead to the appearance of trans-2-nonenal (T2N). The invention enables brewers to produce a beer having insignificant levels of stale, T2N-specific off-flavors, even after prolonged storage of the beverage.

    摘要翻译： 根据本发明，提供了具有功能性脂氧合酶（LOX）-1和LOX-2酶的总损失的大麦及其生产的植物产品，例如通过使用在合成脂肪酸 - 双氧合中缺陷的大麦仁制备的麦芽 酶LOX-1和LOX-2。 所述酶分别涉及与亚油酸双加氧相关的9-和13-氢过氧十八碳二烯酸的主要活性。 9-氢过氧十八碳二烯酸代表LOX途径代谢物，通过进一步的酶促或自发反应，可能导致反式-2-壬醛（T2N）的出现。 本发明使酿造商能够生产具有不明显水平的陈年T2N特异性异味的啤酒，即使在饮料长期储存之后。

    Barley with reduced lipoxygenase activity and beverage prepared therefrom
    7.
    发明申请
    Barley with reduced lipoxygenase activity and beverage prepared therefrom 有权
    具有降低的脂氧合酶活性的大麦和由其制备的饮料

    公开(公告)号：US20110318469A1

    公开(公告)日：2011-12-29

    申请号：US13141579

    申请日：2009-12-28

    申请人： Birgitte Skadhauge ,  Finn Lok ,  Klaus Breddam ,  Ole Olsen ,  Lene Molskov Bech ,  Soren Knudsen

    发明人： Birgitte Skadhauge ,  Finn Lok ,  Klaus Breddam ,  Ole Olsen ,  Lene Molskov Bech ,  Soren Knudsen

    IPC分类号： A01H5/00 ,  C12C12/00 ,  C12C1/027 ,  C12C7/00 ,  A01H1/04 ,  C12C1/18

    CPC分类号： A01H5/10 ,  C12C1/18 ,  C12C12/00 ,  C12C2200/01

    摘要： According to the invention, there is provided barley with total loss of functional lipoxygenase (LOX)-1 and LOX-2 enzymes, and plant products produced thereof, such as malt manufactured by using barley kernels defective in the synthesis of the fatty acid-dioxygenating enzymes LOX-1 and LOX-2. Said enzymes account for the principal activities related to dioxygenation of linoleic acid into 9- and 13-hydroperoxy octadecadienoic acid, respectively. 9-Hydroperoxy octadecadienoic acid represents a LOX pathway metabolite, which—through further enzymatic or spontaneous reactions—may lead to the appearance of trans-2-nonenal (T2N). The invention enables brewers to produce a beer having insignificant levels of stale, T2N-specific off-flavors, even after prolonged storage of the beverage.

    摘要翻译： 根据本发明，提供了具有功能性脂氧合酶（LOX）-1和LOX-2酶的总损失的大麦及其生产的植物产品，例如通过使用在合成脂肪酸 - 双氧合中缺陷的大麦仁制备的麦芽 酶LOX-1和LOX-2。 所述酶分别涉及与亚油酸双加氧相关的9-和13-氢过氧十八碳二烯酸的主要活性。 9-氢过氧十八碳二烯酸代表LOX途径代谢物，通过进一步的酶促或自发反应，可能导致反式-2-壬醛（T2N）的出现。 本发明使酿造商能够生产具有不明显水平的陈年T2N特异性异味的啤酒，即使在饮料长期储存之后。