公开(公告)号：US11826425B2

公开(公告)日：2023-11-28

申请号：US16333114

申请日：2017-09-13

Applicant: VERSITECH LIMITED

Inventor： Philip Man Lung Yeung ,  Fuk Woo Jasper Chan ,  Manson Fok ,  Johnson Yiu-Nam Lau ,  Kwok-Yung Yuen

IPC: A61K39/42 ,  A61P31/12 ,  C07K16/40 ,  A61K39/00

CPC classification number: A61K39/42 ,  A61P31/12 ,  C07K16/40 ,  A61K2039/57

Abstract: Enteroviruses, such as EV-A71, have been found to utilize hWARS as a cell surface receptor, with expression ofhWARS rendering cells susceptible to enteroviral infection. Reduction in hWARS cell surface activity reduces susceptibility to enteroviral infection and provides a mode for treatment or prevention of enteroviral infection and its sequelae. Similarly, expression of hWARS in cultured cells and animal models provides models for understanding enteroviral disease mechanisms and the development of vaccines and/or pharmaceuticals for preventing or treating enteroviral disease.

公开(公告)号：US20240124947A1

公开(公告)日：2024-04-18

申请号：US18473956

申请日：2023-09-25

Applicant: Versitech Limited ,  Centre for Virology, Vaccinology and Therapeutics Limited ,  THE HOSPITAL AUTHORITY

Inventor： Kelvin Kai-Wang To ,  Siddharth Sridhar ,  Kwok-Yung Yuen ,  Chik Yan Yip

IPC: C12Q1/70

CPC classification number: C12Q1/701 ,  C12Q2600/16

Abstract: Sequences for detection of SARS-CoV-2 are provided and include example, SEQ ID NOs:4-8. The sequences are useful for amplifying the SARS-CoV-2 nsp8 gene in a sample, using Reverse Transcriptional Loop-Mediated Isothermal Amplification (RT-LAMP) and it is preferably used in combination with a Cas enzyme/sgRNA pair and a reporter nucleic acid.
The disclosed sequences can be use in methods of detecting SARS-CoV-2 nucleic acids in a sample. Generally, the specific gene sequence of SARS-CoV-2 RNA, herein nsp8, is amplified using RT-LAMP. The RT-LAMP products are scanned by the Cas12a-gRNA ribonucleoprotein (RNP) complex. The RNP binds to the specific complementary to gRNA, activating the transcleavage activity of Cas12a. The active Cas12a system cleaves a short ssDNA reporter that is labeled preferably, with a fluorophore and a quencher on either end. Cleavage of the reporter separates the quencher from the fluorophore, and fluorescence that is detectable with the naked eye is generated.

公开(公告)号：US20240050558A1

公开(公告)日：2024-02-15

申请号：US18267931

申请日：2021-12-10

Applicant: Versitech Limited

Inventor： Honglin Chen ,  Pui Wang ,  Zhiwei Chen ,  Kwok-Yung Yuen

IPC: A61K39/215 ,  A61P31/14 ,  C07K14/165 ,  A61K9/00

CPC classification number: A61K39/215 ,  A61P31/14 ,  C07K14/165 ,  A61K9/0043 ,  A61K2039/5254

Abstract: Live attenuated viruses for protection against the novel coronavirus Sars-CoV-2 are provided. The live attenuated chimeric virus strains are based on a live attenuated influenza A or B virus (LAIVA/B), used a master backbone, which includes deletion of the viral virulence element, the NS1 (non-structural protein 1) (DeLNS1), engineered to express one or more antigens of the Sars-CoV-2 (herein, CoV2Ag). The chimeric virus strain is referred to generally herein, as DelNS1-A/B-Sars-CoV-2-CoV2Ag. The DelNS1-A/B-Sars-CoV-2-CoV2Ag strain preferably shows spontaneous cold adaption with preference to grow at 30-33° C. Compositions including the chimeric virus also provided as a co-composition with a LAIVA/B. The DelNS1-A/B-Sars-CoV-2-CoV2Ag strain can be used to protect a subject in need thereof, against a challenge of Sars-CoV-2. The co-compositions can be used to protect a subject in need thereof, against a challenge of Sars-CoV-2 and influenza A and/or B.

公开(公告)号：US11441198B2

公开(公告)日：2022-09-13

申请号：US16312958

申请日：2017-06-30

Applicant: VERSITECH LIMITED

Inventor： Kai Wang Kelvin To ,  Jie Zhou ,  Johnson Yiu-Nam Lau ,  Kwok-Yung Yuen

IPC: C12Q1/68 ,  C12Q1/70 ,  G16B20/20 ,  G16H50/20 ,  C12Q1/6883 ,  G01N33/569 ,  G16H50/30

Abstract: Compositions and methods are described that provide prognostic determination of the severity of influenza infection based on the PDE3A status of an individual. Impaired PDE3A function is associated with severe symptoms on contracting influenza. Specific SNP mutations are identified that are associated with impaired PDE3A function, and primers and kits are provided that permit identification of these SNPs. Such information can also be combined with other patient data.

公开(公告)号：US20240148820A1

公开(公告)日：2024-05-09

申请号：US18546895

申请日：2022-02-16

Applicant: Versitech Limited ,  Centre for Virology, Vaccinology and Therapeutics Limited

Inventor： Hanjun Zhao ,  Kwok-Yung Yuen

IPC: A61K38/10 ,  A01N63/50 ,  A01P1/00 ,  A61K31/4706 ,  A61P31/14

CPC classification number: A61K38/10 ,  A01N63/50 ,  A01P1/00 ,  A61K31/4706 ,  A61P31/14

Abstract: Broad spectrum antiviral peptides and composition including therapeutically effective amounts of the antiviral peptides along with a pharmaceutically acceptable carrier are provided. The antiviral compositions show a strong broad spectrum antiviral effect, without resulting in viral resistance. Preferably, the antiviral compositions contain P16 or a P16-like peptide which can both inhibit viral fusion and endosomal acidification to prevent viral entry through the endocytic pathway. The antiviral compositions can be used in methods of treating diseases caused by viral infections, particularly respiratory viruses such as enveloped coronaviruses (SARS-CoV-2, SARS-CoV and MERS-CoV), the pandemic A(H1N1)pdm09 virus, avian influenza A(H7N9) virus, and the non-enveloped rhinovirus. The compositions are administered in a subject in need thereof in an effective amount to reduce or prevent viral replication in the subject, and thus reduce one or more symptoms of disease, disorder, or illness associated with virus.