公开(公告)号：US20050182249A1

公开(公告)日：2005-08-18

申请号：US10505377

申请日：2003-02-21

申请人： Victor Lobanenkov ,  Dmitri Loukinov ,  Herbert Morse

发明人： Victor Lobanenkov ,  Dmitri Loukinov ,  Herbert Morse

IPC分类号： A61K39/395 ,  A61K48/00 ,  C07H21/04 ,  C07K14/47 ,  C07K14/82 ,  C07K16/30 ,  C12Q1/68 ,  G01N33/574

CPC分类号： C07K14/47 ,  C07K14/4702 ,  C12Q1/6886 ,  C12Q2600/106 ,  G01N33/57484

摘要： An isolated or purified nucleic acid molecule consisting essentially of a nucleotide sequence encoding a human or a non-human BORIS, or a fragment of either of the foregoing; an isolated or purified nucleic acid molecule consisting essentially of a nucleotide sequence that is complementary to a nucleotide sequence encoding a human or a non-human BORIS, or a fragment of either of the foregoing; a vector comprising such an isolated or purified nucleic acid molecule; a cell comprising such a vector; an isolated or purified polypeptide molecule consisting essentially of an amino acid sequence encoding a human or a non-human BORIS, or a fragment of either of the foregoing; a cell line that produces a monoclonal antibody that is specific for an aforementioned isolated or purified polypeptide molecule; and the monoclonal antibody produced by the cell line; methods of diagnosing a cancer or a predisposition to a cancer in a male or female mammal; a method of prognosticating a cancer in a mammal; a method of assessing the effectiveness of treatment of a cancer in a mammal; a method of treating a mammal prophylactically or therapeutically for a cancer, and a composition comprising a carrier and an inhibitor of BORIS.

摘要翻译： 分离或纯化的核酸分子，主要由编码人或非人BORIS的核苷酸序列或前述任一种的片段组成; 基本上由与编码人或非人BORIS的核苷酸序列互补的核苷酸序列或前述任一种的片段组成的分离或纯化的核酸分子; 包含这种分离或纯化的核酸分子的载体; 包含这种载体的细胞; 基本上由编码人或非人BORIS的氨基酸序列或前述任一种的片段组成的分离或纯化的多肽分子; 产生对上述分离或纯化的多肽分子特异性的单克隆抗体的细胞系; 和由细胞系产生的单克隆抗体; 在男性或女性哺乳动物中诊断癌症或倾向于癌症的方法; 预测哺乳动物癌症的方法; 一种评估哺乳动物癌症治疗有效性的方法; 用于预防或治疗癌症的哺乳动物的方法，以及包含载体和BORIS抑制剂的组合物。

公开(公告)号：US20060057123A1

公开(公告)日：2006-03-16

申请号：US11197221

申请日：2005-08-03

申请人： Catherine Ettinger ,  Peter Lipsky ,  Warren Leonard ,  Rosanne Spolski ,  Herbert Morse

发明人： Catherine Ettinger ,  Peter Lipsky ,  Warren Leonard ,  Rosanne Spolski ,  Herbert Morse

IPC分类号： A61K35/14 ,  C12N5/08

CPC分类号： G01N33/5052 ,  A61K35/17 ,  A61K38/20 ,  A61K2035/124 ,  A61K2039/5158 ,  C12N5/0635 ,  C12N2501/052 ,  C12N2501/23 ,  C12N2501/50 ,  C12N2501/52 ,  G01N2333/54

摘要： A method is disclosed herein for inducing differentiation of a B cell progenitor into a memory B cells and/or a plasma cell. The method includes contacting a population of cells including a mature B cell or a B cell progenitor with an effective amount of IL-21, and isolating memory B cells or plasma cells. In one embodiment, the B cell progenitor is an immature B cell. A method is also disclosed for enhancing an immune response. The method includes contacting a population of cells including a B cell progenitor with an effective amount of IL-21, and isolating memory B cells or plasma cells. The memory B cells and/or the plasma cell are then introduced into the subject to enhance the immune response. A method is also disclosed for treating a subject with a condition comprising a specific deficiency of at least one of memory B cells and plasma cells. A method is disclosed for identifying an agent with a physiological effect on one or more of a memory B cell and a plasma cell differentiation. A method is also disclosed for identifying agents that inhibit an activity of IL-21. Methods are also disclosed for inducing apoptosis of a B cell and for decreasing the number of B cells. A method is also described for producing a B cell hybridoma.

摘要翻译： 本文公开了一种用于诱导B细胞祖细胞分化为记忆B细胞和/或浆细胞的方法。 该方法包括使包含成熟B细胞或B细胞祖细胞的细胞群与有效量的IL-21接触，并分离记忆B细胞或浆细胞。 在一个实施方案中，B细胞祖细胞是未成熟B细胞。 还公开了一种用于增强免疫应答的方法。 该方法包括使包含B细胞祖细胞的细胞群与有效量的IL-21接触，并分离记忆B细胞或浆细胞。 然后将记忆B细胞和/或浆细胞引入受试者以增强免疫应答。 还公开了一种用于治疗患有包含存储B细胞和浆细胞中的至少一种的特定缺陷的病症的受试者的方法。 公开了用于鉴定对存储B细胞和浆细胞分化中的一种或多种具有生理作用的药剂的方法。 还公开了用于鉴定抑制IL-21活性的试剂的方法。 还公开了诱导B细胞凋亡和减少B细胞数量的方法。 还描述了用于产生B细胞杂交瘤的方法。

公开(公告)号：US20070003515A1

公开(公告)日：2007-01-04

申请号：US10579988

申请日：2004-11-18

申请人： Warren Leonard ,  Peter Lipsky ,  Herbert Morse ,  Catherine Ettinger ,  Rosanne Spolski

发明人： Warren Leonard ,  Peter Lipsky ,  Herbert Morse ,  Catherine Ettinger ,  Rosanne Spolski

IPC分类号： A61K38/20 ,  A61K35/14 ,  G01N33/567

CPC分类号： G01N33/5052 ,  A61K35/17 ,  A61K38/20 ,  A61K2035/124 ,  A61K2039/5158 ,  C12N5/0635 ,  C12N2501/052 ,  C12N2501/23 ,  C12N2501/50 ,  C12N2501/52 ,  G01N2333/54

摘要： A method is disclosed herein for inducing differentiation of a B cell progenitor into a memory B cells and/or a plasma cell. The method includes contacting a population of cells including a mature B cell or a B cell progenitor with an effective amount of IL-21, and isolating memory B cells or plasma cells. In one embodiment, the B cell progenitor is an immature B cell. A method is also disclosed for enhancing an immune response. The method includes contacting a population of cells including a B cell progenitor with an effective amount of IL-21, and isolating memory B cells or plasma cells. The memory B cells arid/or the plasma cell are then introduced into the subject to enhance the immune response. A method is also disclosed for treating a subject with a condition comprising a specific deficiency of at least one of memory B cells and plasma cells. A method is disclosed for identifying an agent with a physiological effect on one or more of a memory B cell and a plasma cell differentiation. A method is also disclosed for identifying agents that inhibit an activity of IL-21.

摘要翻译： 本文公开了一种用于诱导B细胞祖细胞分化为记忆B细胞和/或浆细胞的方法。 该方法包括使包含成熟B细胞或B细胞祖细胞的细胞群与有效量的IL-21接触，并分离记忆B细胞或浆细胞。 在一个实施方案中，B细胞祖细胞是未成熟B细胞。 还公开了一种用于增强免疫应答的方法。 该方法包括使包含B细胞祖细胞的细胞群与有效量的IL-21接触，并分离记忆B细胞或浆细胞。 然后将记忆B细胞和/或浆细胞引入受试者以增强免疫应答。 还公开了一种用于治疗患有包含存储B细胞和浆细胞中的至少一种的特定缺陷的病症的受试者的方法。 公开了用于鉴定对存储B细胞和浆细胞分化中的一种或多种具有生理作用的药剂的方法。 还公开了用于鉴定抑制IL-21活性的试剂的方法。

公开(公告)号：US20050193434A1

公开(公告)日：2005-09-01

申请号：US11027868

申请日：2004-12-30

申请人： Warren Leonard ,  Katsutoshi Ozaki ,  Rosanne Spolski ,  Herbert Morse ,  Peter Lipsky ,  Derry Roopenian

发明人： Warren Leonard ,  Katsutoshi Ozaki ,  Rosanne Spolski ,  Herbert Morse ,  Peter Lipsky ,  Derry Roopenian

IPC分类号： A01K67/027 ,  C07K14/54 ,  C07K14/715 ,  C12N20060101 ,  C12N15/85 ,  C12Q1/68 ,  G01N33/53

CPC分类号： C07K14/7155 ,  A01K67/0276 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/0381 ,  C07K14/5406 ,  C12N15/8509

摘要： A transgenic mouse is disclosed herein whose somatic and germ cells comprise a disrupted IL-21 receptor gene, the disruption being sufficient to inhibit the binding of IL-21 to an IL-21 receptor, and a disrupted IL-4 gene, the disruption being sufficient to inhibit the production of IL-4 or the binding of IL-4 to the IL-4 receptor. A mouse homozygous for the disrupted IL-21 receptor gene and homozygous for the disrupted IL-4 gene has diminished B cell function. A method is disclosed for altering a B cell activity. The method includes administering a therapeutically effective amount of an agent that interferes with the interaction of IL-21 with an IL-21 receptor, thereby altering the B cell activity. A method is also disclosed for of treating a subject with Job's disorder or atopic disease. A method is also disclosed for treating or preventing an allergic reaction in a subject. A method is also disclosed for treating a subject with an autoimmune or antibody mediated disorder.

摘要翻译： 本文公开了一种转基因小鼠，其体细胞和生殖细胞包含破坏的IL-21受体基因，所述破坏足以抑制IL-21与IL-21受体的结合以及破坏的IL-4基因，所述破坏是 足以抑制IL-4的产生或IL-4与IL-4受体的结合。 对于破坏的IL-21受体基因纯合的小鼠和破坏的IL-4基因是纯合的，具有减少的B细胞功能。 公开了用于改变B细胞活性的方法。 该方法包括施用治疗有效量的干扰IL-21与IL-21受体相互作用的试剂，由此改变B细胞活性。 还公开了用于治疗患有约瑟病或特应性疾病的受试者的方法。 还公开了用于治疗或预防受试者的过敏反应的方法。 还公开了用自身免疫或抗体介导的病症治疗受试者的方法。