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公开(公告)号：US5620851A
公开(公告)日：1997-04-15
申请号：US323631
申请日：1994-10-17
CPC分类号： G01N33/532 , C07H21/00 , C12Q1/6813 , C12Q1/682 , C12Q1/6867 , C12Q1/703
摘要： This invention relates to the use of functional reporter molecules in the detection and measurement of targets in a sample. The invention is predicated on the utilization of a transcription step between the production of an appropriate reporter molecule and replication based amplification in order to increase the number of detectable species as an indirect reference to target.
摘要翻译： 本发明涉及功能报告分子在检测和测量样品中靶标中的用途。 本发明基于在适当报道分子的产生和基于复制的扩增之间的转录步骤的利用，以便增加可检测物种的数量作为靶标的间接参考。
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公开(公告)号：US5356774A
公开(公告)日：1994-10-18
申请号：US908833
申请日：1992-07-02
IPC分类号： C07H21/00 , C12Q1/68 , C12Q1/70 , G01N33/532
CPC分类号： G01N33/532 , C07H21/00 , C12Q1/6813 , C12Q1/682 , C12Q1/6867 , C12Q1/703
摘要： This invention relates to the use of functional reporter molecules in the detection and measurement of nucleic acid sequences in a sample, as a determination, for example, of pathogenic disease existence or potential. The invention is predicated on the utilization of a transcription step between the production of an appropriate reporter molecule and replication based amplification in order to increase the number of detectable species as an indirect reference to target nucleic acid sequence.
摘要翻译： 本发明涉及功能报告分子在检测和测量样品中的核酸序列中的用途，作为例如致病性疾病存在或潜在的确定。 本发明基于在适当报道分子的产生和基于复制的扩增之间的转录步骤的利用，以便增加作为靶核酸序列的间接参考的可检测物种的数量。
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