-
公开(公告)号：US10153146B2
公开(公告)日：2018-12-11
申请号：US14671199
申请日：2015-03-27
摘要： The invention provides methods, systems and algorithms for identifying high-resolution mass spectra. In some embodiments, an analyte is ionized and analyzed using high-resolution mass spectrometry (MS) at high mass accuracy (such as ≤75 ppm or ≤30 ppm) and the obtained mass spectra are matched with one or more prospective candidate molecules or chemical formulas. The invention provide, for example, methods and systems wherein the possible fragments that can be generated from the candidate molecules or chemical formulas are determined as well as the masses of each of these fragments. The invention provide, for example, methods and systems wherein the high-resolution mass spectra are then compared with the calculated fragment masses for each of the candidate molecules or chemical formula, and the portion of the high-resolution mass spectra that corresponds or can be explained by the calculated fragment masses is determined.
-
公开(公告)号：US10809267B2
公开(公告)日：2020-10-20
申请号：US15611232
申请日：2017-06-01
IPC分类号： G01N33/68 , G01N33/483 , H01J49/00 , H01J49/04 , H01J49/26
摘要： Described herein are mass spectrometry systems and methods which improve the accuracy of isobaric tag-based quantification by alleviating the pervasive problem of precursor interference and co-isolation of impurities through gas-phase purification. During the gas-phase purification, the mass-to-charge ratios of precursor ions within at least a selected range are selectively changed allowing ions having similar unmodified mass-to-charge ratios to be separated before further isolation, fragmentation or analysis.
-
公开(公告)号：US10852306B2
公开(公告)日：2020-12-01
申请号：US15611232
申请日：2017-06-01
IPC分类号： G01N33/68 , G01N33/483 , H01J49/00 , H01J49/04 , H01J49/26
摘要： Described herein are mass spectrometry systems and methods which improve the accuracy of isobaric tag-based quantification by alleviating the pervasive problem of precursor interference and co-isolation of impurities through gas-phase purification. During the gas-phase purification, the mass-to-charge ratios of precursor ions within at least a selected range are selectively changed allowing ions having similar unmodified mass-to-charge ratios to be separated before further isolation, fragmentation or analysis.
-
公开(公告)号：US10273511B2
公开(公告)日：2019-04-30
申请号：US14755213
申请日：2015-06-30
发明人： Rachelle A. S. Lemke , Timothy James Donohue , Joshua J. Coon , Amelia C. Peterson , Michael S. Westphall
摘要： Enzymes for producing non-straight-chain fatty acids, microorganisms comprising the enzymes, and in vivo and in vitro uses of the enzymes. Provided are enzymes capable of producing various non-straight-chain fatty acids, including branched-chain fatty acids, cyclic fatty acids, and furan-containing fatty acids. The enzymes include RSP2144, RSP1091, and RSP1090 from Rhodobacter sphaeroides and homologs thereof. The enzymes can be purified to produce non-straight-chain fatty acids in vitro or expressed in microorganisms to produce non-straight-chain fatty acids in vivo. The microorganisms can be fine-tuned to produce a specific type of non-straight-chain fatty acid by expressing, overexpressing, or deleting the enzymes in various combinations.
-
公开(公告)号：US20150376659A1
公开(公告)日：2015-12-31
申请号：US14755213
申请日：2015-06-30
发明人： Rachelle A.S. Lemke , Timothy James Donohue , Joshua J. Coon , Amelia C. Peterson , Michael S. Westphall
CPC分类号： C12P7/6409 , C12N9/1007 , C12P17/04
摘要： Enzymes for producing non-straight-chain fatty acids, microorganisms comprising the enzymes, and in vivo and in vitro uses of the enzymes. Provided are enzymes capable of producing various non-straight-chain fatty acids, including branched-chain fatty acids, cyclic fatty acids, and furan-containing fatty acids. The enzymes include RSP2144, RSP1091, and RSP1090 from Rhodobacter sphaeroides and homologs thereof. The enzymes can be purified to produce non-straight-chain fatty acids in vitro or expressed in microorganisms to produce non-straight-chain fatty acids in vivo. The microorganisms can be fine-tuned to produce a specific type of non-straight-chain fatty acid by expressing, overexpressing, or deleting the enzymes in various combinations.
摘要翻译： 用于生产非直链脂肪酸的酶，包含酶的微生物以及酶的体内和体外应用。 提供能够产生各种非直链脂肪酸的酶，包括支链脂肪酸，环状脂肪酸和含呋喃的脂肪酸。 酶包括来自球形红细菌的RSP2144，RSP1091和RSP1090及其同系物。 可以纯化酶以在体外产生非直链脂肪酸或在微生物中表达以在体内产生非直链脂肪酸。 通过以各种组合表达，过表达或缺失酶，微生物可被微调以产生特定类型的非直链脂肪酸。
-
-
-
-
搜索结果
5 条记录 (耗时 0.016 秒)
