公开(公告)号：US20240327849A1

公开(公告)日：2024-10-03

申请号：US17632936

申请日：2019-08-05

申请人： Wacker Chemie AG

发明人： Johanna Koch ,  Marian Kujau

IPC分类号： C12N15/70 ,  C07K16/28 ,  C12N9/10 ,  C12N9/48

CPC分类号： C12N15/70 ,  C07K16/2875 ,  C12N9/1074 ,  C12N9/48 ,  C12Y204/01019 ,  C07K2317/14 ,  C07K2317/55 ,  C07K2319/02

摘要： The invention relates to a bacterial strain containing an open reading frame encoding a signal peptide and a recombinant protein under the control of a functional promoter. The bacteria strain contains an additional open reading frame encoding for a signal peptide and a peptidoglycan peptidase under the control of a functional promoter.

公开(公告)号：US12084664B2

公开(公告)日：2024-09-10

申请号：US17262057

申请日：2018-07-24

申请人： WACKER CHEMIE AG

发明人： Tobias Dassler ,  Marian Kujau

IPC分类号： C12N15/70 ,  C12N1/20 ,  C12N15/67 ,  C12R1/19

CPC分类号： C12N15/70 ,  C12N1/20 ,  C12N15/67 ,  C12R2001/19

摘要： An Escherichia coli bacterial strain contains a gene encoding a recombinant protein and an open reading frame of



i) a DNA fragment encoding an N-terminal signal peptide which mediates translocation of the protein into the periplasm,

wherein the N-terminal signal peptide is an amino acid sequence with at least 80% correspondence in relation to SEQ ID No. 2 from amino acids 1 to 20 or is a signal peptide of lipoproteins Pal, NlpI, NlpB or OsmB of Escherichia coli,


linked to
ii) a following DNA sequence (lpp(N)) encoding a lipoprotein (Lpp(N)) which, compared to SEQ ID No. 2 from amino acids 21 to 78, is a different in at most ten amino acids and
iii) a further DNA sequence (lpp(C)) encoding a lipoprotein (Lpp(C)) which, compared to SEQ ID No. 2 from amino acids 21 to 78, is a different in at most ten amino acids.