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    METHODS USING GENETIC PACKAGE DISPLAY FOR DETECTING AND IDENTIFYING PROTEIN-PROTEIN INTERACTIONS THAT FACILITATE INTERNALIZATION AND TRANSGENE EXPRESSION AND CELLS OR TISSUES COMPETENT FOR THE SAME AND METHODS FOR EVOLVING GENE DELIVERY VECTORS
    2.
    发明授权
    METHODS USING GENETIC PACKAGE DISPLAY FOR DETECTING AND IDENTIFYING PROTEIN-PROTEIN INTERACTIONS THAT FACILITATE INTERNALIZATION AND TRANSGENE EXPRESSION AND CELLS OR TISSUES COMPETENT FOR THE SAME AND METHODS FOR EVOLVING GENE DELIVERY VECTORS 失效
    用于检测和鉴定蛋白质 - 蛋白质相互作用的方法，所述蛋白质 - 蛋白质相互作用促进内在化和转移表达和细胞或组织的相互竞争力以及用于产生基因递送载体的方法

    公开(公告)号：US06723512B2

    公开(公告)日：2004-04-20

    申请号：US09866073

    申请日：2001-05-24

    申请人： David Larocca ,  Paul Kassner ,  Andrew Baird

    发明人： David Larocca ,  Paul Kassner ,  Andrew Baird

    IPC分类号： C12Q168

    CPC分类号： C40B40/02 ,  C12N15/1037 ,  C12Q2600/158

    摘要： A genetic package display system and methodology for probing protein-protein interactions that lead to cell transduction, selecting and/or identifying internalizing ligands, target cells and tissues which internalize known or putative ligands, and cell transduction facilitating peptides is provided. A ligand displaying genetic package that carries a selectable marker (e.g., reporter, selection, etc.) and presents a ligand on its surface is utilized to identify internalizing ligands, tranduction facilitating peptides, and/or a variety of cells and tissue types for the ability to be successfully transduced by the ligand displaying genetic package. Also provided are methods for evolving a ligand displaying package to facilitate gene delivery or delivery of any desired agent (e.g., pharmaceutical, polypeptide, peptide, etc.) into a cell and/or targeting cellular compartments such as the nucleus, endosome, chloroplast, mitochondria, etc.

    摘要翻译： 提供了导致细胞转导，选择和/或鉴定内在化配体，靶细胞和内在化已知或推定的配体的组织以及细胞转导促进肽的蛋白质 - 蛋白质相互作用的遗传包装显示系统和方法。 用于显示携带选择标记（例如报告物，选择等）并在其表面上呈现配体的遗传包装的配体用于鉴定内化配体，促进肽的转导和/或多种细胞和组织类型，用于 通过显示遗传包装的配体成功转导的能力。 还提供了用于进化配体显示包以促进基因递送或将任何所需试剂（例如药物，多肽，肽等）递送至细胞和/或靶向细胞，例如细胞核，内体，叶绿体， 线粒体等

    Methods for identifying protein-protein interactions by selective transduction
    3.
    发明授权
    Methods for identifying protein-protein interactions by selective transduction 失效
    通过选择性转导鉴定蛋白质 - 蛋白质相互作用的方法

    公开(公告)号：US06589730B1

    公开(公告)日：2003-07-08

    申请号：US09193445

    申请日：1998-11-17

    申请人： David Larocca ,  Andrew Baird ,  Paul Kassner

    发明人： David Larocca ,  Andrew Baird ,  Paul Kassner

    IPC分类号： C12Q170

    CPC分类号： C40B40/02 ,  A61K47/6901 ,  C07K2319/00 ,  C12N15/1037 ,  C12N2799/021 ,  C12Q1/6811 ,  C12Q1/6897

    摘要： A method for identifying specific protein-protein (i.e., ligand/anti-ligand) interactions that internalize and are detected by transgene expression. A ligand displaying genetic package that carries a reporter or selectable marker and presents a ligand or putative ligand on its surface is utilized to screen cells displaying known or putative anti-ligands for the ability to successfully internalize the ligand displaying genetic package.

    摘要翻译： 用于鉴定内在化并通过转基因表达检测的特异性蛋白质（即配体/抗配体）相互作用的方法。 使用显示遗传包装的携带报告物或选择性标记并在其表面上呈现配体或推定配体的配体来筛选显示已知或推定的抗配体的细胞，以便能够成功地内化显示遗传包的配体。