公开(公告)号：US6133000A

公开(公告)日：2000-10-17

申请号：US087969

申请日：1998-06-01

申请人： Walter Pfefferle ,  Hermann Lotter ,  Heinz Friedrich ,  Wolfgang Degener

发明人： Walter Pfefferle ,  Hermann Lotter ,  Heinz Friedrich ,  Wolfgang Degener

IPC分类号： C12P13/08

CPC分类号： C12P13/08

摘要： A process is disclosed for the fermentative preparation of amino acids, in which an L-lysine producing bacterial strain of the species Corynebacterium glutamicum is cultivated in a nutrient medium and the amino acids can be isolated from the culture medium at the end of fermentation. After the vigorous growth phase, the bacterial culture has at its disposal a smaller quantity of assimilable carbon source than it could metabolize on the basis of the structure of the strain and the quanitity of other necessary supplements provided in the nutrient medium.

摘要翻译： 公开了用于发酵制备氨基酸的方法，其中在营养培养基中培养棒状杆菌谷氨酸棒杆菌种的L-赖氨酸生产菌株，并且可以在发酵结束时从培养基中分离出氨基酸。 在生长旺盛阶段之后，细菌培养物可以根据菌株的结构和营养培养基中提供的其他必需的补充剂的量来代替较少数量的可同化碳源。

公开(公告)号：US5770409A

公开(公告)日：1998-06-23

申请号：US677911

申请日：1996-07-10

申请人： Walter Pfefferle ,  Hermann Lotter ,  Heinz Friedrich ,  Wolfgang Degener

发明人： Walter Pfefferle ,  Hermann Lotter ,  Heinz Friedrich ,  Wolfgang Degener

IPC分类号： C12P13/00 ,  C07K16/00 ,  C12N1/20 ,  C12P13/04 ,  C12P13/08 ,  C12R1/15

CPC分类号： C07K16/00 ,  C12P13/04 ,  C12P13/08

摘要： A process is disclosed for the fermentative preparation of amino acids, in which a strain of the genus Brevibacterium or Corynebacterium producing one or more amino acids is cultivated in a nutrient medium and the amino acids are isolated from the culture fluid at the end of fermentation. After the vigorous growth phase, the bacterial culture has at its disposal a smaller quantity of assimilable carbon source than it could metabolize on the basis of the structure of the strain and the quantity of other necessary supplements provided in the nutrient medium.

摘要翻译： 公开了用于发酵制备氨基酸的方法，其中在营养培养基中培养产生一种或多种氨基酸的短杆菌属或棒状杆菌属的菌株，并且在发酵结束时从培养液中分离氨基酸。 在旺盛的生长阶段之后，细菌培养物可以根据菌株的结构和营养培养基中提供的其他必需的补充剂的数量，使其具有较少量的可同化碳源。

公开(公告)号：US5326441A

公开(公告)日：1994-07-05

申请号：US890602

申请日：1992-07-13

申请人： Ioannis Kirgios ,  Karl Schugerl ,  Wolfgang Degener

发明人： Ioannis Kirgios ,  Karl Schugerl ,  Wolfgang Degener

IPC分类号： B01D61/40 ,  C01G15/00 ,  B01D17/06 ,  B03C5/00 ,  C02F1/48

CPC分类号： C01G15/003

摘要： Process for separating and concentrating gallium from an aqueous solution containing gallium, together with aluminium, zinc and/or copper, charactrized by the following combination of features: (a) preparation of a water-in-oil emulsion consisting of a neutral or alkaline aqueous phase and an organic phase containing an oil-soluble reactant in a homogenizer in a volume ratio of aqueous phase to organic phase of 2:2 to 3:2; (b) dispersion of the water-in-oil emulsion in the aqueous solution containing gallium, which is adjusted to at least 4 mol/l of HCI, in an extractor in a volume ratio of aqueous phase to water-in-oil emulsion of 30:1 to 10:1 until droplets of the water-in-oil emulsion with a diameter less than or equal to 1 mm are obtained; (c) separation of the water-in-oil emulsion from the aqueous continuum and splitting of the emulsion in a reactor with coaxial, double-insulated electrodes in a high-voltage kV/cm and a frequency greater than 50 Hz, the unsplit portion of the water-in-oil emulsion being recycled into step (b); (d) separation of the electrocoalesced organic phase of the water-in-oil emulsion in a gravity separator and recycling of the same into step (a).

摘要翻译： PCT No.PCT / EP90 / 02321 Sec。 371日期：1992年7月13日 102（e）日期1992年7月13日PCT 1990年1月19日PCT PCT。 公开号WO91 / 10621 日期：1991年7月25日。通过以下特征组合，从含有镓的水溶液中分离和浓缩镓的方法以及铝，锌和/或铜特征：（a）制备油包水乳液 由中性或碱性水相和在均化器中含有油溶性反应物的水相与有机相的体积比为2：2至3：2的有机相组成; （b）在萃取器中将油包水乳液分散在含镓的水溶液中，该水溶液被调节至至少4mol / l的HCl，水相体积比与油包水乳液的体积比 30：1至10：1，直到获得直径小于或等于1mm的油包水乳液的液滴; （c）从水相连续体中分离油包水乳液并在具有高压kV / cm和频率大于50Hz的同轴双电绝缘电极的反应器中分离乳液，非分离部分 的油包水乳液被再循环到步骤（b）中; （d）在重力分离器中分离油包水乳液的电聚结有机相，并将其再循环到步骤（a）中。