公开(公告)号：US20230194543A1

公开(公告)日：2023-06-22

申请号：US18053093

申请日：2022-11-07

Applicant: Waters Technologies Corporation

Inventor： Johannes Vissers ,  Thomas McDonald ,  Keith Richardson

IPC: G01N33/68 ,  G01N30/88

CPC classification number: G01N33/6848 ,  G01N30/88 ,  G01N2030/8831

Abstract: Provided herein are methods, techniques and processes for selecting one or more peptides for virus detection (e.g., influenza, SARS-CoV-2) in clinical samples using mass spectrometry. Methods for selecting a combination of peptides to identify one or more disease states in a patient can include: (i) selecting the one or more disease states; (ii) collecting information on proteins associated with the one or more disease states being present within the subject; (ii) in silico digesting individual proteins associated with the one or more disease state to obtain possible workflow peptides; (iv) collecting filtering data associated with the possible workflow peptides; (iv) analyzing the possible workflow peptides for coverage of the one or more disease states and for mass spectrometry detection and resolution; and (iv) selecting the combination of peptides from the possible workflow peptides based on the analyzing step.

公开(公告)号：US11105778B2

公开(公告)日：2021-08-31

申请号：US16098280

申请日：2017-05-04

Applicant: Waters Technologies Corporation

Inventor： Martin Gilar ,  Richard W. Andrews ,  Peyton C. Beals ,  Thomas McDonald ,  Keith Fadgen ,  Jordan Kornfeld ,  Geoff Gerhardt ,  Sylvain Cormier

IPC: G01N30/24 ,  G01N30/06 ,  G01N30/20 ,  G01N30/34 ,  G01N1/38

Abstract: A method for injecting a diluted sample in a chromatography system includes merging a flow of a sample and a flow of a diluent to form a flow of a diluted sample. A dilution ratio of the diluted sample equals a sum of the volumetric flow rates of the sample and the diluent divided by the volumetric flow rate of the sample. The diluted sample is stored in a holding element before injection into a chromatographic system flow. Sample dilution occurs under low pressure relative to the chromatographic flow thereby allowing lower pressure sample and diluent syringes to be used. Other benefits include reduced compressibility and a reduction in leaks due to the lower pressure operation. The method avoids problems associated with manual techniques which can introduce errors due, for example, to loss of sample, sample precipitation and adsorption of sample to vials.

公开(公告)号：US20190170706A1

公开(公告)日：2019-06-06

申请号：US16098280

申请日：2017-05-04

Applicant: Waters Technologies Corporation

Inventor： Martin Gilar ,  Richard W. Andrews ,  Peyton C. Beals ,  Thomas McDonald ,  Keith Fadgen ,  Jordan Kornfeld ,  Geoff Gerhardt ,  Sylvain Cormier

IPC: G01N30/24 ,  G01N30/06

Abstract: A method for injecting a diluted sample in a chromatography system includes merging a flow of a sample and a flow of a diluent to form a flow of a diluted sample. A dilution ratio of the diluted sample equals a sum of the volumetric flow rates of the sample and the diluent divided by the volumetric flow rate of the sample. The diluted sample is stored in a holding element before injection into a chromatographic system flow. Sample dilution occurs under low pressure relative to the chromatographic flow thereby allowing lower pressure sample and diluent syringes to be used. Other benefits include reduced compressibility and a reduction in leaks due to the lower pressure operation. The method avoids problems associated with manual techniques which can introduce errors due, for example, to loss of sample, sample precipitation and adsorption of sample to vials.

公开(公告)号：US11280769B2

公开(公告)日：2022-03-22

申请号：US16678454

申请日：2019-11-08

Applicant: Waters Technologies Corporation

Inventor： Jason Hill ,  Abhijit Tarafder ,  Michael O. Fogwill ,  Thomas McDonald ,  Sylvain Cormier

IPC: G01N30/20 ,  B01D15/40 ,  G01N30/34 ,  G01N30/32 ,  G01N30/02

Abstract: The disclosure relates to a system for liquid sample introduction into a chromatography system. The system includes a syringe, a first valve in fluid communication with the syringe, a second valve in fluid communication with the sample, a vessel located between, and in fluid communication with, the first and second valves, a third valve in fluid communication with the first valve, the second valve and a chromatography column, and a pump in fluid communication with the third valve and a mobile phase. When the valves are in a first position the syringe draws the sample into the vessel. The mobile phase flows to the chromatography column. When the valves are in a second position, a portion of the mobile phase flows into the vessel, mixing with and pressurizing the sample. When the valves are in a third position, the mixed and pressurized sample flows to the chromatography column.

公开(公告)号：US20200150097A1

公开(公告)日：2020-05-14

申请号：US16678454

申请日：2019-11-08

Applicant: Waters Technologies Corporation

Inventor： Jason Hill ,  Abhijit Tarafder ,  Michael O. Fogwill ,  Thomas McDonald ,  Sylvain Cormier

IPC: G01N30/20 ,  B01D15/40 ,  G01N30/34 ,  G01N30/32

Abstract: The disclosure relates to a system for liquid sample introduction into a chromatography system. The system includes a syringe, a first valve in fluid communication with the syringe, a second valve in fluid communication with the sample, a vessel located between, and in fluid communication with, the first and second valves, a third valve in fluid communication with the first valve, the second valve and a chromatography column, and a pump in fluid communication with the third valve and a mobile phase. When the valves are in a first position the syringe draws the sample into the vessel. The mobile phase flows to the chromatography column. When the valves are in a second position, a portion of the mobile phase flows into the vessel, mixing with and pressurizing the sample. When the valves are in a third position, the mixed and pressurized sample flows to the chromatography column.