公开(公告)号：US08801918B2

公开(公告)日：2014-08-12

申请号：US12748918

申请日：2010-03-29

申请人： Wei Qin ,  Daniel S. Cheek ,  Christopher Hobot ,  Kelvin Bonnema ,  Randy Meyer ,  Douglas Dean Nippoldt ,  Vitally G. Sitko ,  Qingshan (Sam) Ye ,  Narayanan Ramamurthy

发明人： Wei Qin ,  Daniel S. Cheek ,  Christopher Hobot ,  Kelvin Bonnema ,  Randy Meyer ,  Douglas Dean Nippoldt ,  Vitally G. Sitko ,  Qingshan (Sam) Ye ,  Narayanan Ramamurthy

IPC分类号： G01N27/333 ,  G01N33/86 ,  A61M1/36

CPC分类号： G01N27/3335 ,  A61M1/3675 ,  G01N27/333 ,  G01N33/86

摘要： Methods and devices for point of care determination of heparin concentration in blood are described. Cartridges including protamine ion sensitive electrodes (ISEs) and reference electrodes and systems for automatically determining heparin concentration in the cartridges are provided. Some systems add blood to a protamine bolus sufficient to bind all heparin, leaving excess protamine. The excess protamine concentration can be determined by measuring the initial slope of the electrode potential rate of change, and comparing the slope to known protamine concentration slope values In some cartridges, an oscillating pressure source moves the blood-protamine mixture back and forth across the protamine ISE. Some systems also use a second blood sample having the heparin removed or degraded to create a blank reference sample. Protamine ISEs can include polyurethane polymer, DNNS ionophore, and NPOE plasticizer. The polyurethane may include hard segments and soft segments, where both hard and soft segments may include cyclic and straight chain aliphatic moieties having essentially no ester or ether groups. Some hard segments may include methylene diphenyl groups. Some reference electrodes have the same polymer, plasticizer, and ionophore as the measurement electrode, but with a different concentration of ionophore.

摘要翻译： 描述了血液中肝素浓度的护理点测定方法和装置。 提供了包括鱼精蛋白离子敏感电极（ISE）和用于自动确定药筒中肝素浓度的参比电极和系统的药盒。 一些系统将血液添加到足以结合所有肝素的鱼精蛋白推注物，留下过量的鱼精蛋白。 可以通过测量电极电位变化率的初始斜率，并将斜率与已知的精蛋白浓度斜率值进行比较来确定过量鱼精蛋白浓度。 在一些药筒中，振荡压力源通过鱼精蛋白ISE来回移动血液 - 鱼精蛋白混合物。 一些系统还使用除去或降解肝素以创建空白参考样品的第二血液样品。 鱼精蛋白ISE可以包括聚氨酯聚合物，DNNS离子载体和NPOE增塑剂。 聚氨酯可以包括硬链段和软链段，其中硬链段和软链段都可以包括基本上没有酯或醚基团的环状和直链脂族部分。 一些硬链段可以包括亚甲基二苯基。 一些参比电极与测量电极具有相同的聚合物，增塑剂和离子载体，但是具有不同浓度的离子载体。

公开(公告)号：US07699966B2

公开(公告)日：2010-04-20

申请号：US11126887

申请日：2005-05-11

申请人： Wei Qin ,  Daniel S. Cheek ,  Christopher Hobot ,  Kelvin Bonnema ,  Randy Meyer ,  Douglas Dean Nippoldt ,  Vitally G. Sitko ,  Qingshan (Sam) Ye ,  Narayanan Ramamurthy

发明人： Wei Qin ,  Daniel S. Cheek ,  Christopher Hobot ,  Kelvin Bonnema ,  Randy Meyer ,  Douglas Dean Nippoldt ,  Vitally G. Sitko ,  Qingshan (Sam) Ye ,  Narayanan Ramamurthy

IPC分类号： G01N27/333

CPC分类号： G01N27/3335 ,  A61M1/3675 ,  G01N27/333 ,  G01N33/86

摘要： Methods and devices for point of care determination of heparin concentration in blood are described. Cartridges including protamine ion sensitive electrodes (ISEs) and reference electrodes and systems for automatically determining heparin concentration in the cartridges are provided. Some systems add blood to a protamine bolus sufficient to bind all heparin, leaving excess protamine. The excess protamine concentration can be determined by measuring the initial slope of the electrode potential rate of change, and comparing the slope to known protamine concentration slope values In some cartridges, an oscillating pressure source moves the blood-protamine mixture back and forth across the protamine ISE. Some systems also use a second blood sample having the heparin removed or degraded to create a blank reference sample. Protamine ISEs can include polyurethane polymer, DNNS ionophore, and NPOE plasticizer. The polyurethane may include hard segments and soft segments, where both hard and soft segments may include cyclic and straight chain aliphatic moieties having essentially no ester or ether groups. Some hard segments may include methylene diphenyl groups. Some reference electrodes have the same polymer, plasticizer, and ionophore as the measurement electrode, but with a different concentration of ionophore.

摘要翻译： 描述了血液中肝素浓度的护理点测定方法和装置。 提供了包括鱼精蛋白离子敏感电极（ISE）和用于自动确定药筒中肝素浓度的参比电极和系统的药盒。 一些系统将血液添加到足以结合所有肝素的鱼精蛋白推注物，留下过量的鱼精蛋白。 过量的鱼精蛋白浓度可以通过测量电极电位变化率的初始斜率来确定，并将斜率与已知的鱼精蛋白浓度斜率值进行比较。在一些药筒中，振荡压力源将血 - 鱼精蛋白混合物来回移动穿过鱼精蛋白 ISE。 一些系统还使用除去或降解肝素以创建空白参考样品的第二血液样品。 鱼精蛋白ISE可以包括聚氨酯聚合物，DNNS离子载体和NPOE增塑剂。 聚氨酯可以包括硬链段和软链段，其中硬链段和软链段都可以包括基本上没有酯或醚基团的环状和直链脂族部分。 一些硬链段可以包括亚甲基二苯基。 一些参比电极与测量电极具有相同的聚合物，增塑剂和离子载体，但是具有不同浓度的离子载体。

公开(公告)号：US20100181210A1

公开(公告)日：2010-07-22

申请号：US12748918

申请日：2010-03-29

申请人： Wei Qin ,  Daniel S. Cheek ,  Christopher Hobot ,  Kelvin Bonnema ,  Randy Meyer ,  Douglas Dean Nippoldt ,  Vitally G. Sitko ,  Qingsham (Sam) Ye ,  Narayanan Ramamurthy

发明人： Wei Qin ,  Daniel S. Cheek ,  Christopher Hobot ,  Kelvin Bonnema ,  Randy Meyer ,  Douglas Dean Nippoldt ,  Vitally G. Sitko ,  Qingsham (Sam) Ye ,  Narayanan Ramamurthy

IPC分类号： G01N33/50 ,  G01N27/26

CPC分类号： G01N27/3335 ,  A61M1/3675 ,  G01N27/333 ,  G01N33/86

摘要： Methods and devices for point of care determination of heparin concentration in blood are described. Cartridges including protamine ion sensitive electrodes (ISEs) and reference electrodes and systems for automatically determining heparin concentration in the cartridges are provided. Some systems add blood to a protamine bolus sufficient to bind all heparin, leaving excess protamine. The excess protamine concentration can be determined by measuring the initial slope of the electrode potential rate of change, and comparing the slope to known protamine concentration slope values In some cartridges, an oscillating pressure source moves the blood-protamine mixture back and forth across the protamine ISE. Some systems also use a second blood sample having the heparin removed or degraded to create a blank reference sample. Protamine ISEs can include polyurethane polymer, DNNS ionophore, and NPOE plasticizer. The polyurethane may include hard segments and soft segments, where both hard and soft segments may include cyclic and straight chain aliphatic moieties having essentially no ester or ether groups. Some hard segments may include methylene diphenyl groups. Some reference electrodes have the same polymer, plasticizer, and ionophore as the measurement electrode, but with a different concentration of ionophore.

摘要翻译： 描述了血液中肝素浓度的护理点测定方法和装置。 提供了包括鱼精蛋白离子敏感电极（ISE）和用于自动确定药筒中肝素浓度的参比电极和系统的药盒。 一些系统将血液添加到足以结合所有肝素的鱼精蛋白推注物，留下过量的鱼精蛋白。 可以通过测量电极电位变化率的初始斜率，并将斜率与已知的精蛋白浓度斜率值进行比较来确定过量鱼精蛋白浓度。 在一些药筒中，振荡压力源通过鱼精蛋白ISE来回移动血液 - 鱼精蛋白混合物。 一些系统还使用除去或降解肝素以创建空白参考样品的第二血液样品。 鱼精蛋白ISE可以包括聚氨酯聚合物，DNNS离子载体和NPOE增塑剂。 聚氨酯可以包括硬链段和软链段，其中硬链段和软链段都可以包括基本上没有酯或醚基团的环状和直链脂族部分。 一些硬链段可以包括亚甲基二苯基。 一些参比电极与测量电极具有相同的聚合物，增塑剂和离子载体，但是具有不同浓度的离子载体。

公开(公告)号：US07422905B2

公开(公告)日：2008-09-09

申请号：US10826994

申请日：2004-04-19

申请人： Cynthia T. Clague ,  Daniel S. Cheek ,  Douglas D. Nippoldt

发明人： Cynthia T. Clague ,  Daniel S. Cheek ,  Douglas D. Nippoldt

IPC分类号： G01N33/86

CPC分类号： G01N11/162 ,  G01N11/14 ,  G01N15/042 ,  G01N15/05 ,  G01N33/4905 ,  G01N2015/0092 ,  Y10T436/111666

摘要： A system and method for determining a coagulation time, e.g., TT, PT, aPTT, and ACT, of a test sample deposited in a test cartridge is disclosed. A cartridge housing having upper and lower major sides and a minor sidewall encloses a test chamber having a test chamber pivot element and is provided with a cartridge port for introducing a test sample into the test chamber. Ferromagnetic agitator vane leaflets extend from an agitator pivot element supported by the test chamber pivot element intermediate the upper and lower major sides for rotational motion. The agitator vane leaflets can be swept, in response to an external magnetic field, through the test sample in the absence of coagulation. A timer is started when the agitator movement is commenced whereupon the agitator moves freely. Resistance to agitator movement due to coagulation is detected, and the coagulation time is measured.

摘要翻译： 公开了一种用于确定沉积在测试盒中的测试样品的凝固时间（例如TT，PT，aPTT和ACT）的系统和方法。 具有上下侧和下侧壁的盒壳体包围具有测试室枢转元件的测试室，并且设置有用于将测试样品引入测试室的盒端口。 铁磁搅拌器叶片叶片从由测试室枢转元件支撑的搅拌器枢轴元件延伸，位于上，下三个主要侧面以用于旋转运动。 在不存在凝结的情况下，搅拌器叶片叶片可以响应于外部磁场扫过测试样品。 当搅拌器运动开始时，搅拌器自由移动，启动定时器。 检测到由于凝结而产生的搅拌器运动阻力，测定凝固时间。

公开(公告)号：US07399637B2

公开(公告)日：2008-07-15

申请号：US10892000

申请日：2004-07-15

申请人： David W. Wright ,  Timothy J. Kriewall ,  Daniel S. Cheek

发明人： David W. Wright ,  Timothy J. Kriewall ,  Daniel S. Cheek

IPC分类号： G01N33/86

CPC分类号： G01N33/4905 ,  G01N11/14 ,  G01N11/16 ,  Y10T436/111666

摘要： A system and method for determining a coagulation time, e.g., TT, PT, aPTT, and ACT, of a blood test sample deposited in a test cartridge is disclosed. A cartridge housing having upper and lower major sides and a minor sidewall encloses a test chamber having a test chamber pivot element and is provided with a cartridge port for introducing a test sample into the test chamber,. Ferromagnetic agitator vane leaflets extend from an agitator pivot element supported by the test chamber pivot element intermediate the upper and lower major sides for rotational motion. The agitator vane leaflets can be swept, in response to an external magnetic field, through the test sample in the absence of coagulation. A timer is started when the agitator movement is commenced whereupon the agitator moves freely. Resistance to agitator movement due to coagulation is detected, and the coagulation time is measured.

摘要翻译： 公开了一种用于确定沉积在测试盒中的血液测试样品的凝固时间（例如TT，PT，aPTT和ACT）的系统和方法。 具有上下侧和下侧壁的盒壳体包围具有测试室枢转元件的测试室，并且设置有用于将测试样品引入到测试室中的盒端口。 铁磁搅拌器叶片叶片从由测试室枢转元件支撑的搅拌器枢轴元件延伸，位于上，下三个主要侧面以用于旋转运动。 在不存在凝结的情况下，搅拌器叶片叶片可以响应于外部磁场扫过测试样品。 当搅拌器运动开始时，搅拌器自由移动，启动定时器。 检测到由于凝结而产生的搅拌器运动阻力，测定凝固时间。