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    Lipase Variants and Polynucleotides Encoding Same
    1.
    发明申请
    Lipase Variants and Polynucleotides Encoding Same 审中-公开
    脂肪酶变体和编码相同的多核苷酸

    公开(公告)号：US20150140588A1

    公开(公告)日：2015-05-21

    申请号：US14124929

    申请日：2012-07-03

    申请人： Werner Besenmatter ,  Allan Svendsen

    发明人： Werner Besenmatter ,  Allan Svendsen

    IPC分类号： C12N9/20

    CPC分类号： C12N9/20 ,  C12Y301/01003

    摘要： The present invention relates to variants with improved activity in an amide-bond reaction. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

    摘要翻译： 本发明涉及具有改进的酰胺键反应活性的变体。 本发明还涉及编码这些变体的多核苷酸; 核酸构建体，载体和包含多核苷酸的宿主细胞; 以及使用这些变体的方法。

    Lipase variants for pharmaceutical use
    4.
    发明授权
    Lipase variants for pharmaceutical use 有权
    用于药物用途的脂肪酶变体

    公开(公告)号：US09029115B2

    公开(公告)日：2015-05-12

    申请号：US13587043

    申请日：2012-08-16

    申请人： Allan Svendsen ,  Michael Skjoet ,  Deborah Yaver ,  Lars Lehmann Hylling Christensen ,  Signe Eskildsen Larsen ,  Nina Lundin ,  Michael Lamsa ,  Peter Colin Gregory

    发明人： Allan Svendsen ,  Michael Skjoet ,  Deborah Yaver ,  Lars Lehmann Hylling Christensen ,  Signe Eskildsen Larsen ,  Nina Lundin ,  Michael Lamsa ,  Peter Colin Gregory

    IPC分类号： C12N9/20 ,  C12N9/14 ,  C12N9/26 ,  C12N9/48 ,  C12Q1/34 ,  C12Q1/40

    CPC分类号： A61K38/465 ,  A61K38/47 ,  A61K38/48 ,  C12N9/20

    摘要： The pharmaceutical use of lipases related to the Thermomyces lanuginosus (Humicola lanuginosa) lipase comprising amino acids 1-269 of SEQ ID NO: 2, optionally in combination with a protease and/or an amylase. Examples of medical indications are: Treatment of digestive disorders, pancreatic exocrine insufficiency (PEI), pancreatitis, cystic fibrosis, diabetes type I, and/or diabetes type II. The lipases of the invention have, e.g., an improved digestion performance in vitro, an improved activity at a pH in the neutral range, an improved stability at low pH, and are stable against protease-degradation, and/or are stable in the presence of pepsin and bile salts. The invention also relates to methods of determining digestion performance in vitro of lipases, as well as to certain novel variants of the lipase of T. lanuginosus.

    摘要翻译： 与包含SEQ ID NO：2的氨基酸1-269的Thermomyces lanuginosus（Humicola lanuginosa）脂肪酶相关的脂肪酶的药物用途，任选地与蛋白酶和/或淀粉酶组合。 医学适应症的例子有：消化系统疾病，胰腺外分泌功能不全（PEI），胰腺炎，囊性纤维化，I型糖尿病和/或II型糖尿病的治疗。 本发明的脂肪酶例如在体外具有改善的消化性能，在中性范围内的pH值改善的活性，在低pH下的改进的稳定性，并且对蛋白酶降解是稳定的，和/或在存在下是稳定的 的胃蛋白酶和胆汁盐。 本发明还涉及确定脂肪酶的体外消化性能的方法以及T.lanuginosus的脂肪酶的某些新颖变体。

    Protease variants
    7.
    发明授权
    Protease variants 有权
    蛋白酶变体

    公开(公告)号：US08563289B2

    公开(公告)日：2013-10-22

    申请号：US12758943

    申请日：2010-04-13

    申请人： Allan Svendsen ,  Stefan Minning

    发明人： Allan Svendsen ,  Stefan Minning

    IPC分类号： C12N9/64 ,  C07H21/04 ,  C11D3/386

    CPC分类号： C12N9/54 ,  C07K2299/00

    摘要： The present invention relates to methods for producing variants of a parent RP-II protease and the variants having altered properties as compared to the parent RP-II protease.

    摘要翻译： 本发明涉及用于产生亲本RP-II蛋白酶的变体的方法和与亲本RP-II蛋白酶相比具有改变的性质的变体。

    Alpha-amylase variants
    8.
    发明授权
    Alpha-amylase variants 有权
    α-淀粉酶变体

    公开(公告)号：US08465957B2

    公开(公告)日：2013-06-18

    申请号：US13478512

    申请日：2012-05-23

    申请人： Allan Svendsen ,  Soren Kjaerulff ,  Henrik Bisgaard-Frantzen ,  Carsten Andersen

    发明人： Allan Svendsen ,  Soren Kjaerulff ,  Henrik Bisgaard-Frantzen ,  Carsten Andersen

    IPC分类号： C12N9/28

    CPC分类号： C12N9/2414 ,  C07H21/04 ,  C11D3/38609 ,  C11D3/38618 ,  C12N9/2417

    摘要： The invention relates to a variant of a parent Termamyl-like alpha-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased.

    摘要翻译： 本发明涉及亲本的类戊酰样α-淀粉酶的变体，其包含2,3,4,5或6个/位置的突变。 这些变体在高温下（相对于亲本）具有增加的稳定性。 本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体，携带本发明的DNA构建体的重组表达载体，用本发明的DNA构建体转化的细胞，使用 本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤，纺织品退浆，淀粉液化，包含本发明的α-淀粉酶变体的洗涤剂添加剂，手动或自动洗碗洗涤剂组合物，其包含α-淀粉酶变体 本发明提供一种产生母体类戊酰样α-淀粉酶的变体的方法，该变体表现出增加。

    Lipase Variants for Pharmaceutical Use
    9.
    发明申请
    Lipase Variants for Pharmaceutical Use 审中-公开
    药用脂肪酶变体

    公开(公告)号：US20120308543A1

    公开(公告)日：2012-12-06

    申请号：US13587043

    申请日：2012-08-16

    申请人： Allan Svendsen ,  Michael Skjoet ,  Deborah Yaver ,  Lars Lehmann Hylling Christensen ,  Signe Eskildsen Larsen ,  Nina Lundin ,  Michael Lamsa ,  Peter Colin Gregory

    发明人： Allan Svendsen ,  Michael Skjoet ,  Deborah Yaver ,  Lars Lehmann Hylling Christensen ,  Signe Eskildsen Larsen ,  Nina Lundin ,  Michael Lamsa ,  Peter Colin Gregory

    IPC分类号： A61K38/54 ,  A61P3/00 ,  A61P3/10 ,  C12N9/20 ,  A61P1/00

    CPC分类号： A61K38/465 ,  A61K38/47 ,  A61K38/48 ,  C12N9/20

    摘要： The pharmaceutical use of lipases related to the Thermomyces lanuginosus (Humicola lanuginosa) lipase comprising amino acids 1-269 of SEQ ID NO: 2, optionally in combination with a protease and/or an amylase. Examples of medical indications are: Treatment of digestive disorders, pancreatic exocrine insufficiency (PEI), pancreatitis, cystic fibrosis, diabetes type I, and/or diabetes type II. The lipases of the invention have, e.g., an improved digestion performance in vitro, an improved activity at a pH in the neutral range, an improved stability at low pH, an are stable against protease-degradation, and/or are stable in the presence of pepsin and bile salts. The invention also relates to methods of determining digestion performance in vitro of lipases, as well as to certain novel variants of the lipase of T. lanuginosus.

    摘要翻译： 与包含SEQ ID NO：2的氨基酸1-269的Thermomyces lanuginosus（Humicola lanuginosa）脂肪酶相关的脂肪酶的药物用途，任选地与蛋白酶和/或淀粉酶组合。 医学适应症的例子有：消化系统疾病，胰腺外分泌功能不全（PEI），胰腺炎，囊性纤维化，I型糖尿病和/或II型糖尿病的治疗。 本发明的脂肪酶具有例如体外改进的消化性能，在中性范围内的pH值改善的活性，在低pH下的改进的稳定性，对蛋白酶降解是稳定的，和/或在存在时是稳定的 的胃蛋白酶和胆汁盐。 本发明还涉及确定脂肪酶的体外消化性能的方法以及T.lanuginosus的脂肪酶的某些新颖变体。