Screening Method
    1.
    发明申请
    Screening Method 审中-公开
    筛选方法

    公开(公告)号:US20120141630A1

    公开(公告)日:2012-06-07

    申请号:US12278004

    申请日:2007-02-06

    IPC分类号: A21D8/04 C12Q1/44

    摘要: Lipolytic enzymes which improve the properties of dough or baked products generally have a high activity towards lipids which are capable of forming a hexagonal phase, and a screening method was developed on this basis. The improved properties may include a larger loaf volume, an improved shape factor, an improved crumb structure, reduced dough stickiness, improved dough stability and/or improved tolerance towards extended proofing. The advantageous lipid-degrading enzymes have higher activity towards MGDG and/or unsaturated phosphatidyl ethanolamines (APE, PE) than towards DGDG, PC, PS, PI, PG, saturated phosphatidyl ethanolamines, ALPE and/or triglicerides.

    摘要翻译: 改善面团或烘焙产品性质的脂肪分解酶通常对能够形成六方相的脂质具有高活性,并且在此基础上开发了筛选方法。 改进的性能可以包括更大的面包体积,改进的形状因子,改进的面包屑结构,减少的面团粘性,改善的面团稳定性和/或改进的对延伸打样的耐受性。 有利的脂质降解酶对于MGDG和/或不饱和磷脂酰乙醇胺(APE,PE)具有比对DGDG,PC,PS,PI,PG,饱和磷脂酰乙醇胺,ALPE和/或三甘草酸酯更高的活性。

    CGTASE VARIANTS
    3.
    发明申请
    CGTASE VARIANTS 审中-公开

    公开(公告)号:US20100183766A1

    公开(公告)日:2010-07-22

    申请号:US12729877

    申请日:2010-03-23

    IPC分类号: A21D2/00 C12N9/10 C07H21/04

    摘要: The inventors have developed a method of modifying the amino acid sequence of a CGTase to obtain variants. The variants may form linear oligosaccharides as an initial product by starch hydrolysis and a reduced amount of cyclodextrin and may be useful for anti-staling in baked products. The method is based on a comparison of three-dimensional (3D) structures of the CGTase with the structure of a maltogenic alpha-amylase where one or both models includes a substrate. The invention also provides novel CGTase variants.

    摘要翻译: 发明人已经开发出修饰CGTase的氨基酸序列以获得变体的方法。 变体可以通过淀粉水解形成线性寡糖作为初始产物,并减少量的环糊精,并且可用于烘焙产品中的抗老化。 该方法基于CGTase的三维(3D)结构与麦芽糖α-淀粉酶的结构的比较,其中一个或两个模型包括底物。 本发明还提供了新的CGTase变体。

    Method for selecting lipolytic enzyme
    7.
    发明授权
    Method for selecting lipolytic enzyme 有权
    选择脂肪分解酶的方法

    公开(公告)号:US08309147B2

    公开(公告)日:2012-11-13

    申请号:US10538710

    申请日:2003-12-11

    IPC分类号: A23F3/16

    摘要: A method of screening lipolytic enzymes is used to identify candidates for a baking additive which can improve the properties of a baked product when added to the dough. The screening method involves contacting the enzyme with N-acyl phosphatidyl ethanolamine (APE) or N-acyl lyso-phosphatidyl ethanolamine (ALPE), and detecting hydrolysis of an ester bond in the APE or ALPE.

    摘要翻译: 使用筛选脂肪分解酶的方法来鉴定烘烤添加剂的候选物,其可以在加入面团中时改善烘焙产品的性能。 筛选方法包括使酶与N-酰基磷脂酰乙醇胺(APE)或N-酰基溶血磷脂酰乙醇胺(ALPE)接触,并检测APE或ALPE中酯键的水解。