公开(公告)号：US20060101539A1

公开(公告)日：2006-05-11

申请号：US11266880

申请日：2005-11-04

申请人： William Ainley ,  Cory Leatherman Larsen ,  Min Lu ,  Liu Shen ,  Pon Jayakumar ,  Robbi Garrison ,  Dayakar Pareddy ,  Jeffrey Beringer

发明人： William Ainley ,  Cory Leatherman Larsen ,  Min Lu ,  Liu Shen ,  Pon Jayakumar ,  Robbi Garrison ,  Dayakar Pareddy ,  Jeffrey Beringer

IPC分类号： C12N5/04 ,  C12N15/82 ,  A01H1/00

CPC分类号： A01N3/00 ,  A01N1/02 ,  A01N1/0278 ,  C12N5/04

摘要： The present invention relates to methods for the cryopreservation of transformed and non-transformed cells. Also provided by the subject invention are methods of recovering cells that have been cryopreserved. Cultures of cells that have been successfully recovered from cryopreservation are also provided.

摘要翻译： 本发明涉及用于冷冻保存转化和未转化细胞的方法。 本发明还提供了回收已被冷冻保存的细胞的方法。 还提供了从冷冻保存中成功回收的细胞培养物。

公开(公告)号：US20050155119A1

公开(公告)日：2005-07-14

申请号：US11031896

申请日：2005-01-07

申请人： Pon Jayakumar ,  Nicole Hopkins ,  Joseph Petolino ,  Dayakar Pareddy

发明人： Pon Jayakumar ,  Nicole Hopkins ,  Joseph Petolino ,  Dayakar Pareddy

IPC分类号： A01H4/00 ,  C12N5/00 ,  C12N5/04 ,  C12N15/82 ,  A01H1/00 ,  A01H5/00

CPC分类号： A01H4/008 ,  A01H4/005 ,  C12N5/0025 ,  C12N5/04 ,  C12N15/8201 ,  C12N15/8214

摘要： The subject invention provides totipotent, chlorophyllous, cell cultures of maize. In addition, the methods of producing such cultures are applicable to other related species, including cereals such as rice, oats, barley, and heat. The subject cultures are valuable for herbicide studies, studies for enhancing photosynthesis, and genetic manipulation, such as plastid transformation. The methods of the subject invention are capable of providing high percentages of totipotent cells. These cells are capable of sustained cell division and are competent for regeneration over long periods; they provide high-quality target tissue for nuclear and organelle transformation. The invention also describes methods for the introduction of heterologous DNA into the chloroplast genome. The present invention also provides methods, vectors, and gene constructs for enhancing expression of a recombinant nucleic acid sequence in transgenic plants and plant tissues.

摘要翻译： 本发明提供了全能的，叶绿素，玉米的细胞培养物。 此外，生产这种培养物的方法适用于其他相关物种，包括谷物如大米，燕麦，大麦和热。 主题培养物对于除草剂研究，增强光合作用的研究和遗传操作如质体转化是有价值的。 本发明的方法能够提供高百分比的全能细胞。 这些细胞能够持续细胞分裂，并且能够长时间再生; 它们为核和细胞器转化提供高质量的靶组织。 本发明还描述了将异源DNA引入叶绿体基因组的方法。 本发明还提供用于增强转基因植物和植物组织中重组核酸序列表达的方法，载体和基因构建体。