公开(公告)号：US20230363376A1

公开(公告)日：2023-11-16

申请号：US18311418

申请日：2023-05-03

申请人： Burnett Stephens KELLY

发明人： Burnett Stephens KELLY

IPC分类号： A01N1/02

CPC分类号： A01N1/0247 ,  A01N1/0278 ,  A61B2017/00969

摘要： An apparatus for cleaning transplant organs comprising an organ perfusion circuit and perfusion solution filtration circuit that removes virus and bacteria. The perfusion pump circuit has a cassette for containing perfusion fluid and an organ and a perfusion pump to circulate perfusion fluid in and out of the cassette. A perfusion solution filtering device has a filter and a pump coupled to the cassette to pump perfusion fluid out of the cassette, through the filter, and back into the cassette.

公开(公告)号：US20190183114A1

公开(公告)日：2019-06-20

申请号：US16322159

申请日：2017-08-04

申请人： INSERM (Institut National de la Sante et de la Recherche Medicale) ,  Universite de Poitiers ,  Centre Hospitalier Universitaire de Poitiers

发明人： Thierry HAUET ,  Sylvain LE PAPE ,  Raphael THUILLIER, II

IPC分类号： A01N1/02 ,  C12N15/113

CPC分类号： A01N1/0278 ,  A01N1/0226 ,  A01N1/0242 ,  C12N15/113

摘要： The present invention relates to methods and compositions for the preservation of organs. The inventors showed that therapeutic intervention during cold ischemia could significantly alter outcome, suggesting active mechanisms taking place. More particularly, the inventors demonstrated that Unfolded Protein Response could be a critical pathway underlying the relationship between cold ischemia time and graft outcome, highlighting the potential for UPR-based therapeutics to improve transplantation efficiency. In particular, the present invention relates to an activator of PERK-ATF4 pathway, an inhibitor of the ATF6 pathway and an inhibitor of the RNase activity of IRE1α for use in the maintain of organ viability during cold ischemia.

公开(公告)号：US09977012B2

公开(公告)日：2018-05-22

申请号：US14523114

申请日：2014-10-24

申请人： LIFELINE SCIENTIFIC, INC.

发明人： Kelvin G. M. Brockbank ,  Lia Campbell

IPC分类号： C12N5/07 ,  C12N5/0775 ,  A01N1/00 ,  G01N33/50 ,  A01N1/02 ,  F25D25/00 ,  F25D31/00 ,  C12N5/00

CPC分类号： G01N33/5008 ,  A01N1/0205 ,  A01N1/0231 ,  A01N1/0278 ,  C12N5/0068 ,  C12N2533/52 ,  C12N2533/54 ,  F25D25/00 ,  F25D31/005

摘要： A method for cryopreservation of adherent cells attached to a substrate and a method for identifying one or more extracellular matrix (ECM) components and/or matricellular proteins that improves viability and retention of the cryopreserved cells on the substrate. The method for cryopreservation includes treating a substrate with at least one ECM component and/or adding at least one matricellular protein to the substrate or cell culture media, plating the cells on the treated substrate, and cryopreserving the cells. One or more ECM components and/or matricellular proteins that improves cell viability and retention can be identified by evaluating the cells that have been thawed from the cryopreservation temperature to determine cell viability and retention.

公开(公告)号：US20170260500A1

公开(公告)日：2017-09-14

申请号：US15528503

申请日：2015-11-20

申请人： VIRGIN HEALTH BAK QSTP-LLC ,  QATA FOUNDATON FOR EDUCATION,SCIENCE AND COMMUNITY DEVELOPMENT

发明人： Chris GOODMAN ,  Kourosh SAEB-PARSY

IPC分类号： C12N5/073 ,  A01N1/02

CPC分类号： C12N5/0605 ,  A01N1/0221 ,  A01N1/0242 ,  A01N1/0278 ,  A61K35/00

摘要： A method of preparing isolated body tissue (10) for cryogenic storage, the method comprising a step of actively infusing the body tissue (10) with a cryoprotectant (20). Additionally, a kit for preparing umbilical cord tissue (10) for cryogenic storage, comprising a cryoprotectant (20) and infusion means (22) for actively infusing the umbilical cord tissue (10) with the cryoprotectant (20).

公开(公告)号：US20170071192A1

公开(公告)日：2017-03-16

申请号：US15277843

申请日：2016-09-27

申请人： Vanderbilt University ,  The United States Government as Represented by the Department of Veterans Affairs

发明人： Colleen M. BROPHY ,  Padmini KOMALAVILAS ,  Joyce CHEUNG-FLYNN ,  Kyle M. HOCKING ,  Susan S. EAGLE

IPC分类号： A01N1/02 ,  A61K35/44

CPC分类号： A01N1/0226 ,  A01N1/0247 ,  A01N1/0278 ,  A61K31/185 ,  A61K35/44 ,  A61M39/06

摘要： The leading cause of graft failure is the subsequent development of intimal hyperplasia, which represents a response to injury that is thought to involve smooth muscle proliferation, migration, phenotypic modulation, and extracellular matrix (ECM) deposition. Surgical techniques typically employed for vein harvest—stretching the vein, placing the vein in low pH, solutions, and the use of toxic surgical skin markers—are shown here to cause injury. The invention therefore provides for non-toxic surgical markers than also protect against stretch-induced loss of functional viability, along with other additives. Devices and compositions for reducing physical stress or protecting from the effects flowing therefrom, also are provided.

摘要翻译： 移植失败的主要原因是内膜增生的随后发展，其代表对损伤的反应，其被认为涉及平滑肌增生，迁移，表型调节和细胞外基质（ECM）沉积。 通常用于静脉收缩的手术技术 - 拉伸静脉，将静脉置于低pH值，溶液和使用毒性手术皮肤标记物 - 这里显示为造成损伤。 因此，本发明提供了无毒手术标记物，同时也防止了其它添加剂的拉伸诱导的功能活性丧失。 还提供了用于降低物理应力或防止其从其流出的影响的装置和组合物。

公开(公告)号：US09562219B2

公开(公告)日：2017-02-07

申请号：US14142448

申请日：2013-12-27

申请人： SOTIO a.s.

发明人： Jitka Fucikova ,  Lenka Koci ,  Katerina Pokorna ,  Iva Truxova ,  Irena Moserova ,  Daniela Rozkova ,  Radek Spisek

IPC分类号： C12N5/09 ,  A61K35/13 ,  A61K39/00 ,  A01N1/02

CPC分类号： A61K39/0011 ,  A01N1/0221 ,  A01N1/0278 ,  A61K35/13 ,  A61K2039/5152 ,  C12N5/0693

摘要： Described herein is a reliable method for preparing a potent vaccine useful for immunotherapy comprising the step of cryopreserving a population of cells undergoing immunogenic cell death, and using such cells to activate dendritic cells for use in immunotherapy. In a specific embodiment, the method comprises cryopreserving cancer cells undergoing cell death, which can be used to prepare a pharmaceutical composition for immunotherapy against cancer.

摘要翻译： 本文描述的是制备用于免疫治疗的有效疫苗的可靠方法，包括冷冻保存经历免疫原性细胞死亡的细胞群的步骤，以及使用这样的细胞激活用于免疫治疗的树突状细胞。 在具体实施方案中，该方法包括冷冻保存经历细胞死亡的癌细胞，其可用于制备用于免疫治疗癌症的药物组合物。

公开(公告)号：US20160249602A1

公开(公告)日：2016-09-01

申请号：US15054476

申请日：2016-02-26

申请人： Matthew Sherman ,  Robert Kaiser

发明人： Matthew Sherman ,  Robert Kaiser

IPC分类号： A01N1/02

CPC分类号： A01N1/021 ,  A01N1/0278

摘要： The present invention relates to a novel method for the preparation of a pooled or mixed population of cryopreserved cells (e.g. hepatocytes). In particular, the invention entails the rapid thaw of cells (e.g. hepatocytes), donated from a single individual, which are mixed to create a heterogeneous population and then cryopreserved. The invention also concerns preparations of multi-cryopreserved cells to increase viability prior to immediate use. The process entails reducing exposure to chemical and physical stresses to increase the resultant number of viable cells.

摘要翻译： 本发明涉及一种制备冷冻保存细胞（例如肝细胞）的合并或混合群体的新方法。 特别地，本发明需要从单个个体捐赠的细胞（例如肝细胞）的快速解冻，其被混合以产生异质群体，然后冷冻保存。 本发明还涉及多次冷冻保存的细胞的制备以在立即使用之前增加活力。 该过程需要减少暴露于化学和物理应力以增加所产生的活细胞数量。

公开(公告)号：US20150264918A1

公开(公告)日：2015-09-24

申请号：US14671771

申请日：2015-03-27

申请人： The Department of Veteran Affairs

发明人： Waleed H. HASSANEIN ,  Shukri KHURI ,  Michael D. CRITTENDEN ,  Vladimir BIRJINUIK

IPC分类号： A01N1/02

CPC分类号： A01N1/0247 ,  A01N1/02 ,  A01N1/021 ,  A01N1/0278

摘要： Compositions, methods, systems/devices and media are provided for maintaining a harvested organ in a functioning and viable state prior to implantation. The organ perfusion apparatus includes a preservation chamber for storing the organ during the preservation period. A perfusion circuit is provided having a first line for providing an oxygenated fluid to the organ, and a second line for carrying depleted fluid away from the organ. The perfusion apparatus also includes a device operably associated with the perfusion circuit for maintaining the organ at a substantially normothermic temperature.

摘要翻译： 提供组合物，方法，系统/装置和介质，用于在植入之前将收获的器官保持在功能和可行状态。 器官灌注装置包括在保存期间存储器官的保存室。 提供了一种灌注回路，其具有用于向器官提供含氧流体的第一管线和用于将耗尽的流体携带离开器官的第二管线。 灌注装置还包括可操作地与灌注回路相关联的装置，用于将器官维持在基本上正常的体温温度。

公开(公告)号：US20150184130A1

公开(公告)日：2015-07-02

申请号：US14142448

申请日：2013-12-27

申请人： SOTIO a.s.

发明人： Jitka Fucikova ,  Lenka Koci ,  Katerina Pokorna ,  Iva Truxova ,  Irena Moserova ,  Daniela Rozkova ,  Radek Spisek

IPC分类号： C12N5/09 ,  A61K35/13 ,  A61K39/00

CPC分类号： A61K39/0011 ,  A01N1/0221 ,  A01N1/0278 ,  A61K35/13 ,  A61K2039/5152 ,  C12N5/0693

摘要： Described herein is a reliable method for preparing a potent vaccine useful for immunotherapy comprising the step of cryopreserving a population of cells undergoing immunogenic cell death, and using such cells to activate dendritic cells for use in immunotherapy. In a specific embodiment, the method comprises cryopreserving cancer cells undergoing cell death, which can be used to prepare a pharmaceutical composition for immunotherapy against cancer.

摘要翻译： 本文描述的是制备用于免疫治疗的有效疫苗的可靠方法，包括冷冻保存经历免疫原性细胞死亡的细胞群的步骤，以及使用这样的细胞激活用于免疫治疗的树突状细胞。 在具体实施方案中，该方法包括冷冻保存经历细胞死亡的癌细胞，其可用于制备用于免疫治疗癌症的药物组合物。

公开(公告)号：US20140220547A1

公开(公告)日：2014-08-07

申请号：US14188993

申请日：2014-02-25

申请人： The United States Government as Represented by the Department of Veterans Affairs ,  VANDERBILT UNIVERSITY

发明人： Colleen M. BROPHY ,  Padmini KOMALAVILAS ,  Joyce CHEUNG-FLYNN ,  Kyle M HOCKING ,  Susan S EAGLE

IPC分类号： A01N1/02

CPC分类号： A01N1/0226 ,  A01N1/0247 ,  A01N1/0278 ,  A61K31/185 ,  A61K35/44 ,  A61M39/06

摘要： The leading cause of graft failure is the subsequent development of intimal hyperplasia, which represents a response to injury that is thought to involve smooth muscle proliferation, migration, phenotypic modulation, and extracellular matrix (ECM) deposition. Surgical techniques typically employed for vein harvest—stretching the vein, placing the vein in low pH, solutions, and the use of toxic surgical skin markers—are shown here to cause injury. The invention therefore provides for non-toxic surgical markers than also protect against stretch-induced loss of functional viability, along with other additives. Devices and compositions for reducing physical stress or protecting from the effects flowing therefrom, also are provided.

摘要翻译： 移植失败的主要原因是内膜增生的随后发展，其代表对损伤的反应，其被认为涉及平滑肌增生，迁移，表型调节和细胞外基质（ECM）沉积。 通常用于静脉收缩的手术技术 - 拉伸静脉，将静脉置于低pH值，溶液和使用毒性手术皮肤标记物 - 这里显示为造成损伤。 因此，本发明提供了无毒手术标记物，同时也防止了其它添加剂的拉伸诱导的功能活性丧失。 还提供了用于降低物理应力或防止其从其流出的影响的装置和组合物。