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    Methods for reducing adventitious agents and toxins and cell culture reagents produced thereby
    1.
    发明授权
    Methods for reducing adventitious agents and toxins and cell culture reagents produced thereby 有权

    公开(公告)号：US06627426B2

    公开(公告)日：2003-09-30

    申请号：US09576900

    申请日：2000-05-23

    申请人： William C. Biddle ,  Richard M. Fike ,  Barbara M. Dadey ,  Thomas E. Bulera

    发明人： William C. Biddle ,  Richard M. Fike ,  Barbara M. Dadey ,  Thomas E. Bulera

    IPC分类号： C12N100

    CPC分类号： A61L2/0094 ,  A61L2/20 ,  F26B3/12

    摘要： The present invention relates generally to a method to reduce, substantially reduce, inactivate or eliminate adventitious agents and/or toxins in a sample, particuarly in nutritive media, media supplements, media subgroups and buffer formulations. Specifically, the present invention provides powdered nutritive media, media supplements and media subgroups produced by the methods of the invention, particuarly cell culture media supplements (including powdered sera such as powdered fetal bovine serum (FBS)). The invention further provides powdered buffer formulations produced by the methods of the invention. The invention also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these nutritive media, media supplements, media subgroups and buffer formulations. The invention also relates to methods for producing storage stable cells having reduced, substantially reduced or eliminated adventitious agents or toxins.