公开(公告)号：US20130045508A1

公开(公告)日：2013-02-21

申请号：US13526930

申请日：2012-06-19

Applicant: Winfried Edelmann ,  Yongwei Zhang

Inventor： Winfried Edelmann ,  Yongwei Zhang

IPC: C12N15/64

CPC classification number: C12N15/66 ,  C12N15/10 ,  C12N15/64

Abstract: Methods are disclosed for assembling a plurality of double-stranded DNA fragments into DNA molecules in a single in vitro recombination reaction comprising contacting the plurality of double-stranded DNA fragments with a bacterial extract derived from a RecA deficient bacterial strain so as to assemble the plurality of DNA fragments into DNA molecules.

Abstract translation: 公开了用于在单个体外重组反应中将多个双链DNA片段组装成DNA分子的方法，包括使多个双链DNA片段与来自RecA缺陷细菌菌株的细菌提取物接触，以便组装多个 的DNA片段进入DNA分子。

公开(公告)号：US08609374B2

公开(公告)日：2013-12-17

申请号：US13526930

申请日：2012-06-19

Applicant: Winfried Edelmann ,  Yongwei Zhang

Inventor： Winfried Edelmann ,  Yongwei Zhang

IPC: C12P19/34 ,  C12P21/06 ,  C12N15/64

CPC classification number: C12N15/66 ,  C12N15/10 ,  C12N15/64

Abstract: Methods are disclosed for assembling a plurality of double-stranded DNA fragments into DNA molecules in a single in vitro recombination reaction comprising contacting the plurality of double-stranded DNA fragments with a bacterial extract derived from a RecA deficient bacterial strain so as to assemble the plurality of DNA fragments into DNA molecules.

Abstract translation: 公开了用于在单个体外重组反应中将多个双链DNA片段组装成DNA分子的方法，包括使多个双链DNA片段与来自RecA缺陷细菌菌株的细菌提取物接触，以便组装多个 的DNA片段进入DNA分子。

公开(公告)号：US06794147B1

公开(公告)日：2004-09-21

申请号：US09991099

申请日：2001-11-21

Applicant: Jeffrey W. Pollard ,  Winfried Edelmann ,  Paula E. Cohen ,  Burkhard Kneitz ,  Panos Stevis ,  Raju S. Kucherlapati

Inventor： Jeffrey W. Pollard ,  Winfried Edelmann ,  Paula E. Cohen ,  Burkhard Kneitz ,  Panos Stevis ,  Raju S. Kucherlapati

IPC: G01N3353

CPC classification number: G01N33/6875 ,  G01N2500/00

Abstract: An animal, e.g., transgenic mouse, in which the MSH4 gene is misexpressed. The animal is useful for screening treatments for a number of conditions. Methods for identifying contraceptive agents are also described.

Abstract translation: 动物，例如转基因小鼠，其中MSH4基因被错误表达。 该动物可用于多种病症的筛选治疗。 还描述了用于鉴定避孕药的方法。

公开(公告)号：US07211375B1

公开(公告)日：2007-05-01

申请号：US09658734

申请日：2000-09-11

Applicant: Winfried Edelmann ,  Richard D. Kolodner ,  Jeffrey W. Pollard ,  Raju S. Kucherlapati

Inventor： Winfried Edelmann ,  Richard D. Kolodner ,  Jeffrey W. Pollard ,  Raju S. Kucherlapati

IPC: C12Q1/00 ,  C12Q1/68 ,  G01N33/48

CPC classification number: C12N15/8509 ,  A01K67/0275 ,  A01K67/0276 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/03 ,  A61K38/00 ,  C07K14/82

Abstract: An animal, e.g., transgenic mouse, in which the MSH5 gene is misexpressed. The animal is useful for screening treatments for a number of conditions. Methods for identifying contraceptive agents are also described.

Abstract translation: 动物，例如转基因小鼠，其中MSH5基因被错误表达。 该动物可用于多种病症的筛选治疗。 还描述了用于鉴定避孕药的方法。

公开(公告)号：US20050153922A1

公开(公告)日：2005-07-14

申请号：US11002557

申请日：2004-12-01

Applicant: Winfried Edelmann ,  Richard Kolodner ,  Jeffrey Pollard ,  Raju Kucherlapati

Inventor： Winfried Edelmann ,  Richard Kolodner ,  Jeffrey Pollard ,  Raju Kucherlapati

IPC: A01K67/027 ,  A61K38/00 ,  C07K14/82 ,  C12N15/85 ,  G01N33/15 ,  G01N33/50 ,  A61K48/00

CPC classification number: C12N15/8509 ,  A01K67/0275 ,  A01K67/0276 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/03 ,  A61K38/00 ,  C07K14/82

Abstract: An animal, e.g., transgenic mouse, in which the MSH5 gene is misexpressed. The animal is useful for screening treatments for a number of conditions. Methods for identifying contraceptive agents are also described.

Abstract translation: 动物，例如转基因小鼠，其中MSH5基因被错误表达。 该动物可用于多种病症的筛选治疗。 还描述了用于鉴定避孕药的方法。

公开(公告)号：US06444873B1

公开(公告)日：2002-09-03

申请号：US09469636

申请日：1999-12-22

Applicant: Winfried Edelmann ,  Richard D. Kolodner ,  Jeffrey W. Pollard ,  Raju S. Kucherlapati

Inventor： Winfried Edelmann ,  Richard D. Kolodner ,  Jeffrey W. Pollard ,  Raju S. Kucherlapati

IPC: A01K6700

CPC classification number: C12N15/8509 ,  A01K67/0275 ,  A01K67/0276 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/03 ,  A61K38/00 ,  C07K14/82

Abstract: An animal, e.g., transgenic mouse, in which the MSH5 gene is misexpressed. The animal is useful for screening treatments for a number of conditions. Methods for identifying contraceptive agents are also described.

Abstract translation: 动物，例如转基因小鼠，其中MSH5基因被错误表达。 该动物可用于多种病症的筛选治疗。 还描述了用于鉴定避孕药的方法。